No abstract

Ten oral verruciform xanthomas were studied using an immunoperoxidase stain for S‐100 protein. All cases exhibited positively stained dendritic cells among the mononuclear inflammatory cell infiltrate at the base of the lesions and to a lesser extent among the “foam cells”. The foam cells were, however, negative for S‐100 staining. We suggest that, based on these findings, verruciform xanthomas belong to a new category of “non‐X histiocytoses” in which the presence of Langerhans cells suggests an immunologic pathogenesis.

Serum antibodies to breast tumor antigen(s) and circulating autoantibodies were tested in 175 patients with various stages of carcinoma of the breast, followed for a mean period of 51 months. Antibodies to surface membrane and to cytoplasmic antigens of autologous and allogeneic tumor cells were measured. Peripheral lymphocyte count and skin reaction to six recall antigens were also tested. Patients with metastatic disease had significantly lower prevalence of antibodies to autologous tumor cells and lower total lymphocyte count than patients with early breast cancer. Patients with locally advanced disease (greater than or equal to 4 positive axillary nodes) had the highest frequency of anti-tumor antibodies, the second highest lymphocyte count, but with the lowest prevalance of autoantibodies. Presence or absence of anti-tumor or autoantibody did not correlate with results of skin tests or other standard blood tests. Among patients with locally advanced or metastatic breast cancer, those who had a positive skin test or whose lymphocyte count was 1500 to 2500 per cu mm had significantly better 5-year absolute survival rates (p = 0.04, p = 0.002, respectively). This study suggests that in patients with locally advanced or metastatic breast cancers, skin test reactivity and optimal peripheral lymphocyte count may be useful prognostic indicators. In contrast, neither the presence of anti-tumor antibodies to membrane or cytoplasmic antigens, nor the presence of autoantibodies, correlates with prognosis in patients with early or late breast cancers.

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Human skin grown in tissue culture medium for periods of up to 18 wk undergoes characteristic morphological changes. After an initial period of degeneration, new foci of epidermal cells arise at the dermo-epidermal junction and by rapid proliferation these cells spread out to form a complete second epidermal layer beneath the degenerating original strata. Stratification occurs in this new epidermis with incomplete keratinisation. The individual keratinocytes show ultrastructural similarities with fetal cells. There is a loss of complexity of the cytoplasm typified by a marked reduction in the numbers of keratin filaments and a decrease in the numbers of desmosomal contact areas. In addition, the formation of cilia and accumulation of glycogen in the cell cytoplasm are characteristic. The cytoplasm of the basal cells contains numerous polyribosomes and there is evidence of synthetic activity as illustrated by the proliferation of rough-surfaced endoplasmic reticulum and Golgi complex. Fluorescent and EM immunocytochemical staining with an anti-fetal antiserum demonstrates the development of fetal substances on the surface of cells during the regeneration stage occurring in the cultured skins. The significance of these observations concerning transplantability of cultured tissues is discussed.