Heterologous Antisera-Enhanced Hemolysin in Sera and Tissue Extracts

Abstract

Summary A technique is described for determining serum and tissue hemolysin titers in microliter quantities using a modification of the Jerne technique for hemolysin plaque-forming cells. The technique permits the visualization of augmented hemolysis resulting from the addition of rabbit anti-mouse sera. The enhanced antibody is a γ-globulin as characterized by several criteria. A γ-globulin or 7 S hemolysin was demonstrated in serum collected from animals injected 5 days previously with sheep red blood cells. A comparison of hemolysin plaque-forming cells and serum hemolysin titers indicated that peak levels of the developed hemolysin plaque-forming cells appeared 1 week prior to peak serum levels of developed hemolysin.