Heterologous Co-expression of accA, fabD, and Thioesterase Genes for Improving Long-Chain Fatty Acid Production in Pseudomonas aeruginosa and Escherichia coli

Lee, Sunhee; Jeon, Eui Yong; Jung, Yeontae; Lee, Jinwon

doi:10.1007/s12010-012-9644-5

Cited by 13 publications

(4 citation statements)

References 26 publications

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“…To illustrate, A. thaliana has two thioesterases, namely AtFatA and AtFatB, with high specificity towards C16:1 and C16 fatty acids, respectively [105]. Thioesterases, that could not be sorted into FatA or FatB are listed separately and show a high effect on C16:1 (Table 4) [31,111].…”

Section: Thioesterasesmentioning

confidence: 99%

“…In addition, E. coli has already been engineered to produce (shorter chain) MUFAs [7,26,27]. While strongly dependent on strain and growth conditions, the fatty acid profile of E. coli generally consists of palmitic acid (C16), palmitoleic acid (C16:1 Δ9 ), myristic acid (C14), stearic acid (C18) and cisvaccenic acid (C18:1 Δ11 ), with C16 fatty acids making up around 70% of the total fatty acids [28][29][30][31]. In terms of fatty acid unsaturation, the basal ratio of saturated to unsaturated fatty acids ranges from one to two, depending on environmental variables [32].…”

Section: Introductionmentioning

confidence: 99%

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Strategies to Enhance the Biosynthesis of Monounsaturated Fatty Acids in Escherichia coli

Matthay

Schalck

Verstraeten

et al. 2023

Biotechnol Bioproc E

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Fueled by a variety of industrial applications ranging from bioplastics to cosmetics and pharmaceuticals, the global demand for unsaturated fatty acids is steadily rising. Most of these applications build on monounsaturated fatty acids with a chain length of 16 or 18 carbon atoms, rendering these compounds valuable industrial assets. While of high industrial interest, monounsaturated fatty acids with a chain length of 8 to 12 carbon atoms are hard to produce using conventional chemical or plant-based production ways. As a consequence, these compounds are expensive and not readily available for large-scale industrial applications. Recent advances in metabolic engineering have put forward microbes as cost-efficient factories to produce numerous chemical compounds. In this respect, the model organism Escherichia coli is considered an interesting species as it can grow on various feedstocks and a plethora of genetic information is available, facilitating expression of exogenous enzymes. For the purpose of shifting the fatty acid pool towards monounsaturated fatty acid, thioesterases and desaturases represent suitable candidate enzymes. The former stop chain elongation, reacting on acyl-chains of specific chain length and saturation level, whereas the latter directly target fatty acids to convert them into unsaturated analogues. In this review we summarize thioesterases and desaturases that have been introduced in E. coli to enrich unsaturated fatty acids. Furthermore, we discuss advantages of using bacteria for the production of designer compounds including but not limited to medium-chain monounsaturated fatty acids.

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Section: Thioesterasesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Strategies to Enhance the Biosynthesis of Monounsaturated Fatty Acids in Escherichia coli

Matthay

Schalck

Verstraeten

et al. 2023

Biotechnol Bioproc E

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“…The diversity and specificity of thioesterases have been examined and classified based on their activities and characteristics (Jing et al, ). Overexpression of native E. coli thioesterases tesA and tesB (Choi and Lee, ; Lennen et al, ; Steen et al, ), as well as heterologous thioesterases from C. camphorum (Liu et al, ; Lu et al, ), U. californica (Choi and Lee, ; Lennen et al, ), R. communis (Li et al, ; Zhang et al, ), J. curcus (Zhang et al, ), S. pyogenes (Lee et al, ), and A. baylyi (Zheng et al, ) has been identified to overproduce FAs with tailored carbon chain length. Optimal expression of plant thioesterases in E. coli guided by predictions of the ribosomal binding sites (Zhang et al, ) as well as discoveries of new thioesterases, such as a recently identified E. coli thioesterase gene, fadM , involved in the β‐oxidation pathway (Dellomonaco et al, ), was shown to improve medium‐chain FA production.…”

Section: Classical Metabolic Engineeringmentioning

confidence: 99%

Systems metabolic engineering design: Fatty acid production as an emerging case study

Tee

Chowdhury

Maranas

et al. 2014

Biotech & Bioengineering

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Increasing demand for petroleum has stimulated industry to develop sustainable production of chemicals and biofuels using microbial cell factories. Fatty acids of chain lengths from C6 to C16 are propitious intermediates for the catalytic synthesis of industrial chemicals and diesel-like biofuels. The abundance of genetic information available for Escherichia coli and specifically, fatty acid metabolism in E. coli, supports this bacterium as a promising host for engineering a biocatalyst for the microbial production of fatty acids. Recent successes rooted in different features of systems metabolic engineering in the strain design of high-yielding medium chain fatty acid producing E. coli strains provide an emerging case study of design methods for effective strain design. Classical metabolic engineering and synthetic biology approaches enabled different and distinct design paths towards a high-yielding strain. Here we highlight a rational strain design process in systems biology, an integrated computational and experimental approach for carboxylic acid production, as an alternative method. Additional challenges inherent in achieving an optimal strain for commercialization of medium chain-length fatty acids will likely require a collection of strategies from systems metabolic engineering. Not only will the continued advancement in systems metabolic engineering result in these highly productive strains more quickly, this knowledge will extend more rapidly the carboxylic acid platform to the microbial production of carboxylic acids with alternate chain-lengths and functionalities.

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“…This type II FAs pathway is the primary pathway for fatty acid metabolism in bacteria and plant cells [ 24 ]. Bacillus can produce a variety of cellular metabolites through the type II FAs pathway, including linear and branched chain fatty acids [ 25 ] with different chain lengths [ 26 ] and saturations [ 24 ]. The type II FAs pathway can be divided into the initial and elongation stages.…”

Section: Introductionmentioning

confidence: 99%

Soybean Oil Regulates the Fatty Acid Synthesis II System of Bacillus amyloliquefaciens LFB112 by Activating Acetyl-CoA Levels

Cheng

Zhang

et al. 2023

Microorganisms

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[Background] Bacillus LFB112 is a strain of Bacillus amyloliquefaciens screened in our laboratory. Previous studies found that it has a strong ability for fatty acid metabolism and can improve the lipid metabolism of broilers when used as feed additives. [Methods] This study aimed to confirm the fatty acid metabolism of Bacillus LFB112. Sterilized soybean oil (SSO) was added to the Beef Peptone Yeast (BPY) medium, and its effect on fatty acid content in the supernatant and bacteria, as well as expression levels of genes related to fatty acid metabolism, were studied. The control group was the original culture medium without oil. [Results] Acetic acid produced by the SSO group of Bacillus LFB112 decreased, but the content of unsaturated fatty acids increased. The 1.6% SSO group significantly increased the contents of pyruvate and acetyl-CoA in the pellets. Furthermore, the mRNA levels of enzymes involved in the type II fatty acid synthesis pathway of FabD, FabH, FabG, FabZ, FabI, and FabF were up-regulated. [Conclusions] Soybean oil increased the content of acetyl-CoA in Bacillus LFB112, activated its type II fatty acid synthesis pathway, and improved the fatty acid metabolism level of Bacillus LFB112. These intriguing results pave the way for further investigations into the intricate interplay between Bacillus LFB112 and fatty acid metabolism, with potential applications in animal nutrition and feed additive development.

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Heterologous Co-expression of accA, fabD, and Thioesterase Genes for Improving Long-Chain Fatty Acid Production in Pseudomonas aeruginosa and Escherichia coli

Cited by 13 publications

References 26 publications

Strategies to Enhance the Biosynthesis of Monounsaturated Fatty Acids in Escherichia coli

Strategies to Enhance the Biosynthesis of Monounsaturated Fatty Acids in Escherichia coli

Systems metabolic engineering design: Fatty acid production as an emerging case study

Soybean Oil Regulates the Fatty Acid Synthesis II System of Bacillus amyloliquefaciens LFB112 by Activating Acetyl-CoA Levels

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