Heterologous expression and biophysical characterization of a mesophilic tannase following manganese nanoparticle immobilization

Dutta, Nalok; Miraz, Shahriar Md.; Khan, Muhammad Usman; Karekar, Supriya Charuhas; Usman, Muhammad; Khan, Shahbaz Manzoor; Amin, Usman; Rebezov, Мaksim; Shariati, Mohammad Ali; Thiruvengadam, Muthu

doi:10.1016/j.colsurfb.2021.112011

Cited by 8 publications

(5 citation statements)

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“…Manganese oxide nanoparticles attract the interest of researchers due to unique physical, chemical, biological, and photocatalytic properties [1][2][3][4][5][6][7][8][9][10], ensuring its widespread use for the production of components applied in supercapacitors [11][12][13] as catalysts for the degradation of organic dyes [14][15][16] and for removal of heavy metal ions from water [17,18]. Manganese oxide nanoparticles and composites are widely used in medicine for the detection, diagnosis and treatment of cancer cells [19][20][21][22][23][24][25][26][27], and targeted chemotherapy [28].…”

Section: Introductionmentioning

confidence: 99%

Effect of MnxOy Nanoparticles Stabilized with Methionine on Germination of Barley Seeds (Hordeum vulgare L.)

et al. 2023

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The aim of this research was to study the effect of MnxOy nanoparticles stabilized with L-methionine on the morphofunctional characteristics of the barley (Hordeum vulgare L.) crop. MnxOy nanoparticles stabilized with L-methionine were synthesized using potassium permanganate and L-methionine. We established that MnxOy nanoparticles have a diameter of 15 to 30 nm. According to quantum chemical modeling and IR spectroscopy, it is shown that the interaction of MnxOy nanoparticles with L-methionine occurs through the amino group. It is found that MnxOy nanoparticles stabilized with L-methionine have positive effects on the roots and seedling length, as well as the seed germination energy. The effect of MnxOy nanoparticles on Hordeum vulgare L. seeds is nonlinear. At a concentration of 0.05 mg/mL, there was a statistically significant increase in the length of seedlings by 68% compared to the control group. We found that the root lengths of samples treated with MnxOy nanoparticle sols with a concentration of 0.05 mg/mL were 62.8%, 32.7%, and 158.9% higher compared to samples treated with L-methionine, KMnO4, and the control sample, respectively. We have shown that at a concentration of 0.05 mg/mL, the germination energy of seeds increases by 50.0% compared to the control sample, by 10.0% compared to the samples treated with L-methionine, and by 13.8% compared to the samples treated with KMnO4.

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Section: Introductionmentioning

confidence: 99%

Effect of MnxOy Nanoparticles Stabilized with Methionine on Germination of Barley Seeds (Hordeum vulgare L.)

et al. 2023

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“…According to Luoma and Rainbow (2005) anthropogenic Cadmium pollution is nearly 31 times greater than natural sources, with humans introducing 5.6-38x10 6 kg of Cd into the soil each year, and they have adverse effects (Benvenga et al, 2020;Dourado et al, 2020;Dutta et al, 2021;Balali-Mood et al, 2021;Bandeira et al, 2022;Ohiagu et al, 2022) with different mechanisms.…”

Section: Sources Of Hmsmentioning

confidence: 99%

Heavy Metal Toxicity and Remediation in Human and Agricultural Systems: An Updated Review

Melebary¹

2023

AAVS

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Heavy metals (HMs) are harmful and lethal at negligible levels and non-biodegradable in the typical ecosystem and constitutes animal, human and environmental hazards. They are divided into toxic metals like Lead, Cadmium, Arsenic, etc. and essential elements like copper, zinc, manganese, iron, nickel and chromium. Additionally, could be categorized into two groups based on the natural and anthropogenic sources releasing origins. Population and industrial expansion led to food contamination with HMs. Poisonous metals can be transferred from irrigation water to agricultural soils, agricultural operations, air pollution, animal feed, and packaging materials. Toxic metals are non-biodegradable, non-thermos degradable, and exceedingly stable in the ecosystem; as a result, they quickly build in various foods. Metal pollution of many foods, including agricultural commodities, and animal protein sources such as fish, milk, meat, and eggs, poses a hazard to food safety and security. Toxic metal pollution of irrigation water, agricultural soils, plants, and animals result in their integration into the food chain, posing a health hazard to humans. Most metals are harmful to animals and humans and accumulate in several organs like the skeleton, hepatic tissue, spleen, and renal tissues. Metals have a deleterious impact on the production of plants and animals. As a result, several remediation strategies have become necessary to limit the hazardous HMs pathway into the food chain and the human body. Metal nanoparticles are employed in beneficial applications, although they are associated with specific hazards.

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“…Despite the previously mentioned benefits, the enzyme-catalyzed transformation of tannin using tannase still faces several key challenges. For example, the complicated purification process, high production cost, insufficient enzyme stability, limited enzyme selectivity or specificity, low tolerance in the presence of inhibitors, finite application conditions, and inability to recycle after every use of free forms make this approach unsustainable for the long-term usage in industrial applications. , In this situation, immobilization of enzymes on a solid support is regarded as an effective strategy to overcome the above-mentioned limitations. − Stable supports for tannase immobilization include calcium alginate beads, − chitosan, manganese nanoparticles, magnetic nanoparticles, multiwalled carbon nanotubes, and silica oxide nanoparticles . The immobilization yield and reusability have been improved to a certain extent, but some major drawbacks remain.…”

Section: Introductionmentioning

confidence: 99%

“…1,3 In this situation, immobilization of enzymes on a solid support is regarded as an effective strategy to overcome the above-mentioned limitations. 20−22 Stable supports for tannase immobilization include calcium alginate beads, 23−25 chitosan, 26 manganese nanoparticles, 27 magnetic nanoparticles, 28 multiwalled carbon nanotubes, 29 and silica oxide nanoparticles. 30 The immobilization yield and reusability have been improved to a certain extent, but some major drawbacks remain.…”

Section: ■ Introductionmentioning

confidence: 99%

Construction of a Tannase-Immobilized Magnetic Graphene Oxide/Polymer Nanobiocatalyst with Enhanced Enzyme Stability for High-Efficiency Transformation of Tannins

Huan

Liu

et al. 2022

ACS Sustainable Chem. Eng.

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Highly efficient biotransformation of natural compounds into sustainable biochemical products has attracted great attention. The integration of nanoscience and biotechnology provides attractive solutions for this purpose. Herein, we report the fabrication of a nanobiocatalyst employing a magnetic graphene oxide/polymer nanocomposite as a robust carrier for immobilizing the tannase enzyme, which catalyzes the bioconversion of tannin into gallic acid and glucose. Attributed to the covalent immobilization and propitious interface properties of the coated polymers comprising polyethylenimine and sodium hyaluronate, the nanobiocatalyst is stable without compromising the enzymatic activity. The nanobiocatalyst exhibits 91.8% activity of original tannase and a high enzyme bound amount of 356.8 mg g −1 . The stability tests at variable temperatures (30−80 °C) and under pH conditions (4.0−9.0), various inhibitors, and a long-term storage process (25 days) reveal that the heterofunctional support and surface microenvironment facilitate better stability, adaptability, and tolerance ability of the nanobiocatalyst modified with a multicomponent polymer in comparison to the free enzyme and the nanobiocatalyst modified with the monocomponent polyethylenimine. The nanobiocatalyst maintains 94.2% of its initial activity after 10 consecutive uses and is 100% recoverable by applying an external magnet. Moreover, the nanobiocatalyst is used to hydrolyze 96.5 and 95.1% tannins in extracts from Chinese Torreya grandis testa and cake, respectively. These results establish the practicability of magnetic graphene oxide-based supports for immobilizing tannase and the promising application of immobilized tannase for efficient tannin hydrolysis.

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Heterologous expression and biophysical characterization of a mesophilic tannase following manganese nanoparticle immobilization

Cited by 8 publications

References 36 publications

Effect of MnxOy Nanoparticles Stabilized with Methionine on Germination of Barley Seeds (Hordeum vulgare L.)

Effect of MnxOy Nanoparticles Stabilized with Methionine on Germination of Barley Seeds (Hordeum vulgare L.)

Heavy Metal Toxicity and Remediation in Human and Agricultural Systems: An Updated Review

Construction of a Tannase-Immobilized Magnetic Graphene Oxide/Polymer Nanobiocatalyst with Enhanced Enzyme Stability for High-Efficiency Transformation of Tannins

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