Heterologous expression and characterization of a recombinant thermophilic arylsulfatase from Thermotoga maritima

Lee, Dong‐Geun; Shin, Jung-Hae; Jeon, Myong Je; Lee, Sang-Hyeon

doi:10.1007/s12257-013-0094-x

Cited by 15 publications

(6 citation statements)

References 25 publications

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“…Desulfation of agars has also been reported in several marine bacteria strains: P. carrageenovora (Kim et al, 2005), Pyrococcus furiosus (Jung et al, 2012), Thermotoga marina (Lee et al, 2013), Marinomonas sp. FW-1 (Wang et al, 2015b), and Flammeovirga pacifica (Gao et al, 2015).…”

Section: Biochemically Characterized Marine Polysaccharide Sulfatasesmentioning

confidence: 80%

“…In all cases, the modalities of desulfation were not demonstrated, notably, the position of the eliminated sulfate ester group. Heterologous expression of T. marina sulfatase (Lee et al, 2013) demonstrates that it belongs to the type I family. Interestingly, P. carrageenonvora sulfatase has homology with type III β-lactamase-like sulfatase.…”

Section: Biochemically Characterized Marine Polysaccharide Sulfatasesmentioning

confidence: 99%

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Marine Polysaccharide Sulfatases

Helbert

2017

Front. Mar. Sci.

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Polysaccharides are the most abundant and the most complex organic molecules in the ocean. In contrast to land polysaccharides, many marine polysaccharides are highly sulfated; in particular, the cell walls of macroalgae harbor a high diversity of sulfated polysaccharides (carrageenans, agarans, fucoidans, ulvans, etc.). These sulfated polysaccharides, biosynthesized by macroalgal primary producers, represent an important food source for heterotrophic organisms. Their biodegradation requires a set of enzymes that can cleave the glycosidic linkages of the carbohydrate backbone (called glycoside hydrolases) and the sulfate ester groups (called polysaccharide sulfatases). This review first provides on overview of the current state of knowledge on the classification and mechanisms of sulfatases in general. Then, based on an exploration of marine genomic and metagenomics data that reveals the diversity of carbohydrate sulfatases, it focuses on strategies to predict these sulfatases. In particular, the modularity of sulfatases and their location in marine polysaccharide utilization loci (PUL) provide clues as to their potential substrates and can drive future functional assays. Finally, the review underscores the low number of currently biochemically characterized marine carbohydrate sulfatases (e.g., agarases, carrageenanases, and fucose sulfatases). Bottlenecks encountered in studies on sulfatases likely lie in the difficulties in purifying them and producing them in heterologous systems.

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Section: Biochemically Characterized Marine Polysaccharide Sulfatasesmentioning

confidence: 80%

Section: Biochemically Characterized Marine Polysaccharide Sulfatasesmentioning

confidence: 99%

Marine Polysaccharide Sulfatases

Helbert

2017

Front. Mar. Sci.

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“…Considering its mesophilic marine bacterium origin, PsFucS1 had a surprisingly high thermal reaction optimum at 68 °C (at pH 6.5) and an unusually high thermostability. Three thermostable sulfatases have been described to date, the agar sulfatase from T. maritima with optimal temperature at 80 °C 25 , Atsa from P. aeruginosa with optimal temperature of 57 °C 38 and Ary423 from F. pacifica , with optimal activity at 40 °C, but with a wide temperature range of 30–70 °C 23 . Ary423 retained more than 40% activity at 60 °C after 12 hours 23 , but the stability of PsFucS1 was even higher with a half-life at 68 °C of 12.2 h.…”

Section: Discussionmentioning

confidence: 99%

“…A few prokaryotic polysaccharide-specific type S1 sulfatases have been characterized from marine bacteria such as Pseudoalteromonas carrageenovora 22 , Flammeovirga pacifica 23 , Pyrococcus furiosus 24 and Thermotoga marina 25 with activity on agar, while carrageenan active sulfatases have been found in Pseudoalteromonas atlantica 26 , 27 , Pseudoalteromonas sp. PS47 18 and P. carrageenovora 22 , 28 .…”

Section: Introductionmentioning

confidence: 99%

A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure

Mikkelsen

Hang

Roret

et al. 2021

Sci Rep

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Fucoidans are sulfated, fucose-rich marine polysaccharides primarily found in cell walls of brown seaweeds (macroalgae). Fucoidans are known to possess beneficial bioactivities depending on their structure and sulfation degree. Here, we report the first functional characterization and the first crystal structure of a prokaryotic sulfatase, PsFucS1, belonging to sulfatase subfamily S1_13, able to release sulfate from fucoidan oligosaccharides. PsFucS1 was identified in the genome of a Pseudoalteromonas sp. isolated from sea cucumber gut. PsFucS1 (57 kDa) is Ca2+ dependent and has an unusually high optimal temperature (68 °C) and thermostability. Further, the PsFucS1 displays a unique quaternary hexameric structure comprising a tight trimeric dimer complex. The structural data imply that this hexamer formation results from an uncommon interaction of each PsFucS1 monomer that is oriented perpendicular to the common dimer interface (~ 1500 Å2) that can be found in analogous sulfatases. The uncommon interaction involves interfacing (1246 Å2) through a bundle of α-helices in the N-terminal domain to form a trimeric ring structure. The high thermostability may be related to this unusual quaternary hexameric structure formation that is suggested to represent a novel protein thermostabilization mechanism.

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“…Researchers managed to improve the thermostability of Pseudoalteromonas carrageenovora arylshulphatase developing mutants [ 20 ] and its enzyme activity by directed evolution followed by characterizing the biochemical properties of the selected mutant enzyme, making a promising tool in industrial applications for agar quality improvement [ 21 ]. Arylsulfatases of some bacterial species have been already characterized as Klebsiella pneumoniae [ 22 ], Kluyveromyces lactis [ 23 ], Pseudoalteromonas carrageenovora [ 24 ], Pseudomonas aeruginosa [ 25 ], Salmonella typhimurium [ 26 ], Serratia marcescens [ 10 , 27 ] and Thermotoga maritima [ 28 ]. Arylsulfatase plays an important role in cancer detection [ 29 ] and contributes to the doping analysis and food processing [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Multi-Trait Wheat Rhizobacteria from Calcareous Soil with Biocontrol Activity Promote Plant Growth and Mitigate Salinity Stress

et al. 2021

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Plant growth promoting rhizobacteria (PGPR) can be functional microbial fertilizers and/or biological control agents, contributing to an eco-spirit and safe solution for chemical replacement. Therefore, we have isolated rhizospheric arylsulfatase (ARS)-producing bacteria, belonging to Pseudomonas and Bacillus genus, from durum wheat crop grown on calcareous soil. These isolates harbouring plant growth promoting (PGP) traits were further evaluated in vitro for additional PGP traits, including indole compounds production and biocontrol activity against phytopathogens, limiting the group of multi-trait strains to eight. The selected bacterial strains were further evaluated for PGP attributes associated with biofilm formation, compatibility, salt tolerance ability and effect on plant growth. In vitro studies demonstrated that the multi-trait isolates, Bacillus (1.SG.7, 5.SG.3) and Pseudomonas (2.SG.20, 2.C.19) strains, enhanced the lateral roots abundance and shoots biomass, mitigated salinity stress, suggesting the utility of beneficial ARS-producing bacteria as potential microbial fertilizers. Furthermore, in vitro studies demonstrated that compatible combinations of multi-trait isolates, Bacillus sp. 1.SG.7 in a mixture coupled with 5.SG.3, and 2.C.19 with 5.SG.3 belonging to Bacillus and Pseudomonas, respectively, may enhance plant growth as compared to single inoculants.

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Heterologous expression and characterization of a recombinant thermophilic arylsulfatase from Thermotoga maritima

Cited by 15 publications

References 25 publications

Marine Polysaccharide Sulfatases

Marine Polysaccharide Sulfatases

A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure

Multi-Trait Wheat Rhizobacteria from Calcareous Soil with Biocontrol Activity Promote Plant Growth and Mitigate Salinity Stress

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