Heterologous expression and characterization of a cold-adapted endo-1,4−β−glucanase gene from Bellamya chinensis laeta

Abstract

Highlights ・A cold-adapted endo-1,4--glucanase from Bellamya chinensis laeta (BC-EG70a) was expressed in Pichia pastoris GS115. ・The optimum pH and temperature of rBC-EG70amature and rBC-EG70aCatD were the same (pH 5.5 and 50 o C). ・At lower temperature (30 o C), the specific activity of rBC-EG70amature toward soluble cellulose was 5 to 20-fold higher than those of Trichoderma reesei and Trichoderma viride cellulases. ・rBC-EG70acatD demonstrated higher activity than rBC-EG70amature in the 10%, 15% and 20% (v/v) ethanol solutions. ・It was suggested that rBC-EG70aCatD and rBC-EG70amature are able to apply the SSF process. Heterologous Expression and Characterization of a Cold-Adapted Endo-−−glucanase Gene from Bellamya chinensis laeta:

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