Heterologous expression and characterization of a laccase from Laccaria bicolor in Pichia pastoris and Arabidopsis thaliana

Wang, Bo; Xu, Jing; Fu, Xin; Han, Ho Jae; Gao, Jian‐Jie; Li, Zhenjun; Wang, Limin; Tian, Yongsheng; Peng, Ri‐He; Yao, Qizheng

doi:10.4014/jmb.1808.08042

Cited by 6 publications

(1 citation statement)

References 29 publications

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“…Fungal laccases and bacterial laccases also tend to cause different effects on lignin; a study showed that fungi mineralized kraft lignin, while bacteria partially degraded and modified lignin [46]. In addition, due to their low substrate specificity, laccases have also been applied in many fields such as bioremediation, wood and paper industries [47,48]. The application of laccase is also reflected in the potential of small lignin production.…”

Section: Laccase (Ec 11032)mentioning

confidence: 99%

Lignin Biodegradation and Its Valorization

et al. 2022

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Lignin, a rigid polymer composed of phenolic subunits with high molecular weight and complex structure, ranks behind only cellulose in the contribution to the biomass of plants. Therefore, lignin can be used as a new environmentally friendly resource for the industrial production of a variety of polymers, dyes and adhesives. Since laccase was found to be able to degrade lignin, increasing attention had been paid to the valorization of lignin. Research has mainly focused on the identification of lignin-degrading enzymes, which play a key role in lignin biodegradation, and the potential application of lignin degradation products. In this review, we describe the source, catalytic specificity and enzyme reaction mechanism of the four classes of the lignin-degrading enzymes so far discovered. In addition, the major pathways of lignin biodegradation and the applications of the degradative products are also discussed. Lignin-degrading bacteria or enzymes can be used in combination with chemical pretreatment for the production of value-added chemicals from lignin, providing a promising strategy for lignin valorization.

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Section: Laccase (Ec 11032)mentioning

confidence: 99%

Lignin Biodegradation and Its Valorization

et al. 2022

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Characterization and Degradation of Triphenylmethane Dyes and Their Leuco‐Derivatives by Heterologously Expressed Laccase From Coprinus cinerea

Qian,

Pei,

Wang

et al. 2024

Cell Biochemistry & Function

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Laccase is a copper‐containing polyphenol oxidase that can oxidize phenolic and non‐phenolic organic substrates. In the past decades, laccases had received considerable attention because of the ability to degrade various organic substances. Based on the codon preference of the Pichia pastoris expression system, this study optimized the gene structure of the laccase gene Lcc1 from Coprius cinerea through synthetic biology methods. A new gene Lcc1I was synthesized and heterologously expressed in P. pastoris. After 3 days of cultivation in a shake flask at 30°C, the transformants produced at a yield of 890 mg L−1protein. The highest production level of the recombinant laccase was 2760 U L−1. The molecular mass of the recombinant laccase was estimated at 60 kDa. The enzyme showed highest activity at pH 3.4 and 45°C. It possessed better stability at higher pH and lower temperature condition. Using 2,2'‐azino‐bis‐(3‐ethylbenzothiazoline)−6‐sulphonate (ABTS) as the substrate, the Km and Vmax values were 0.136 mM and 9778 μM min−1 mg−1, respectively. The recombinant laccase could directly oxidize some triphenylmethane dyes like leuco‐crystal violet (LCV) and leuco‐malachite green (LMG). With the help of ABTS mediator, it could oxidize and degrade 77.7% crystal violet (CV) and 79.2% malachite green (MG) within 1 h. Our results indicate that optimization of the laccase gene achieves good expression results in the host system. The dye degradation model constructed in this study may also be applied to the degradation of other organic pollutants and toxic substances, providing new solutions for environmental remediation against the increasingly severe environmental pollution.

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Enzyme Action for Dye Degradation

Dave

Sanghvi

2021

Dye Biodegradation, Mechanisms and Techniques

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Heterologous expression and characterization of a laccase from Laccaria bicolor in Pichia pastoris and Arabidopsis thaliana

Cited by 6 publications

References 29 publications

Lignin Biodegradation and Its Valorization

Lignin Biodegradation and Its Valorization

Characterization and Degradation of Triphenylmethane Dyes and Their Leuco‐Derivatives by Heterologously Expressed Laccase From Coprinus cinerea

Enzyme Action for Dye Degradation

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