Heterologous expression of pepper mild mottle virus coat protein encoding region and its application in immuno-diagnostics

Kumari, Nidhi; Sharma, Vivek; Patel, Priyankaben

doi:10.1007/s13337-020-00597-9

Cited by 5 publications

(4 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of PMMoV CP in the BL21 strain has been reported to be an inclusion body . BL21-gold (DE3) is a highly expressed strain that uses T7 RNA polymerase to initiate vectors for protein expression.…”

Section: Results and Discussionmentioning

confidence: 99%

“…7,8 The main hosts of PMMoV include Solanaceae, Chenopodiaceae, and Amaranthaceae, which can cause local or systematic infections. 3 PMMoV has a highly contagious nature, transmitted mainly through seeds and soil, and can now be infected by friction. 4 PMMoV-infected plants exhibit chlorosis mottling, leaf upward cupping, puckering of leaves, stunting, vein thickening, and mosaic symptoms, and plant growth becomes significantly slow.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Pepper mild mottle virus (PMMoV), which belongs to the genus Tobamovirus of the family Virgaviridae, is highly infectious and poses a great threat to economically important vegetable crops worldwide. − After consumption, PMMoV enters the human body and has infectious activity even after intestinal digestion and excretion. The virus has been reported as a potential virus indicator to detect water environmental pollution. , PMMoV was first identified in the early 1950s in the United States and was first discovered in China in 1994. , The main hosts of PMMoV include Solanaceae, Chenopodiaceae, and Amaranthaceae, which can cause local or systematic infections . PMMoV has a highly contagious nature, transmitted mainly through seeds and soil, and can now be infected by friction .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pepper Mild Mottle Virus Coat Protein as a Novel Target to Screen Antiviral Drugs

Shi

Liu

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Pepper mild mottle virus (PMMoV) has caused serious economic losses to crop production in many countries. The coat protein (CP) of PMMoV is a multifunctional protein proved to be a determining factor in the assignment of virulence type. Therefore, we studied the interaction between drugs and PMMoV CP as a method to screen anti-PMMoV agents. In this study, vanisulfane (6f) exhibited good inactivation activity (68.5%) by biological activity screening. Meanwhile, the green fluorescent protein and PMMoV CP expression changes of vanisulfane against PMMoV were verified by western blot and qRT-PCR experiments. The affinity between vanisulfane and PMMoV CP was predicted to be the best by autodocking and molecular dynamics simulation. PMMoV CP was purified for the first time from the soluble fraction, and the strong affinity between vanisulfane and CP was further verified by interaction experiments. Therefore, this study found that vanisulfane is a potential anti-PMMoV drug targeting PMMoV CP.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pepper Mild Mottle Virus Coat Protein as a Novel Target to Screen Antiviral Drugs

Shi

Liu

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…In addition, C. baccatum (PI 439381-L 3 ) showed systemic necrosis after artificial inoculation, indicating that this line has partial resistance which might protect a plant from the PMMoV infection through soil-borne infection under field conditions (135). Between 2012 and 2017, we conducted a screening of 290 capsicum germplasm varieties to evaluate their resistance to PMMoV P 12 using bioassay and identified two accessions, viz., PI-159236 and PI-260429, which exhibited resistance (76,136).…”

Section: Screening and Breeding For Pmmov Resistance In Capsicummentioning

confidence: 99%

Pepper mild mottle virus: a formidable foe of capsicum production—a review

Kumari,

Sharma,

Patel

et al. 2023

Front. Virol.

Self Cite

View full text Add to dashboard Cite

Viruses are one of the major restraining factors in pepper cultivation globally. Among different viruses, pepper mild mottle virus (PMMoV) is one of the most detrimental plant viruses infecting Capsicum spp. belonging to the genus Tobamovirus and Virgaviridae family. It has a monopartite positive-sense single-stranded RNA genome of 6.35 kb size. On an average, PMMoV results in 15%–40% losses in capsicum fruit yield. However, the incidence of PMMoV can reach as high as 95%, leading to substantial yield losses ranging from 75% to 95%. The virus is transmitted via contact, soil, and seeds rather than via insect vectors. PMMoV, because of its seed-borne nature, now occurs worldwide. PMMoV mainly infects Capsicum spp. under natural conditions; however, it can experimentally be transmitted to other plants species belonging to the families Solanaceae, Cucurbitaceae, Labiatae, Chenopodiaceae, and Plantaginaceae. The resistance to tobamoviruses in capsicum is conferred by L locus. Mutations in the coat protein of PMMoV are responsible for the emergence of L-mediated resistance-breaking pathotypes. The highly contagious nature of the virus, seed transmission behavior, and the emergence of virulence complicate its management through a single approach. Therefore, efforts are directed towards providing a more practical and efficient integrated management solution using the RNA interference approach; exploitation of the L gene for resistance breeding; and the inhibitory potential of natural products, systemic resistance-inducing antagonistic bacteria, and chemically synthesized silver nanoparticles. Markers linked to L alleles have been observed to accelerate capsicum breeding programs through marker-assisted selection. In this study, an attempt has been made to compile the recent developments in PMMoV biology, pathogenic variability, genomic organization, and management strategies.

show abstract

Role of pepper mild mottle virus as a tracking tool for fecal pollution in aquatic environments

Dhakar

Geetanjali

2022

Arch Microbiol

View full text Add to dashboard Cite

Heterologous expression of pepper mild mottle virus coat protein encoding region and its application in immuno-diagnostics

Cited by 5 publications

References 42 publications

Pepper Mild Mottle Virus Coat Protein as a Novel Target to Screen Antiviral Drugs

Pepper Mild Mottle Virus Coat Protein as a Novel Target to Screen Antiviral Drugs

Pepper mild mottle virus: a formidable foe of capsicum production—a review

Role of pepper mild mottle virus as a tracking tool for fecal pollution in aquatic environments

Contact Info

Product

Resources

About