Heterologous Expression of the Chrysanthemum R2R3-MYB Transcription Factor CmMYB2 Enhances Drought and Salinity Tolerance, Increases Hypersensitivity to ABA and Delays Flowering in Arabidopsis thaliana

Shan, Hong; Chen, Sumei; Jiang, Jiafu; Chen, Fadi; Chen, Yu; Gu, Chao; Li, Peiling; Song, Aiping; Zhu, Xirong; Gao, He; Zhou, Guoqin; Li, Ting; Yang, Xue

doi:10.1007/s12033-011-9451-1

Cited by 101 publications

(54 citation statements)

References 68 publications

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“…There were two transgenic lines to exclude the effect of the insertion site on the characteristics of the offspring. Additionally, there was no significant difference between the empty vector lines and wild plants before or after the salt treatment as descried previously Shan et al 2012;Song et al 2014b).…”

Section: Discussionsupporting

confidence: 62%

Chrysanthemum WRKY gene CmWRKY17 negatively regulates salt stress tolerance in transgenic chrysanthemum and Arabidopsis plants

Song

Gao

et al. 2015

Plant Cell Rep

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CmWRKY17 was induced by salinity in chrysanthemum, and it might negatively regulate salt stress in transgenic plants as a transcriptional repressor. WRKY transcription factors play roles as positive or negative regulators in response to various stresses in plants. In this study, CmWRKY17 was isolated from chrysanthemum (Chrysanthemum morifolium). The gene encodes a 227-amino acid protein and belongs to the group II WRKY family, but has an atypical WRKY domain with the sequence WKKYGEK. Our data indicated that CmWRKY17 was localized to the nucleus in onion epidermal cells. CmWRKY17 showed no transcriptional activation in yeast; furthermore, luminescence assay clearly suggested that CmWRKY17 functions as a transcriptional repressor. DNA-binding assay showed that CmWRKY17 can bind to W-box. The expression of CmWRKY17 was induced by salinity in chrysanthemum, and a higher expression level was observed in the stem and leaf compared with that in the root, disk florets, and ray florets. Overexpression of CmWRKY17 in chrysanthemum and Arabidopsis increased the sensitivity to salinity stress. The activities of superoxide dismutase and peroxidase and proline content in the leaf were significantly lower in transgenic chrysanthemum than those in the wild type under salinity stress, whereas electrical conductivity was increased in transgenic plants. Expression of the stress-related genes AtRD29, AtDREB2B, AtSOS1, AtSOS2, AtSOS3, and AtNHX1 was reduced in the CmWRKY17 transgenic Arabidopsis compared with that in the wild-type Col-0. Collectively, these data suggest that CmWRKY17 may increase the salinity sensitivity in plants as a transcriptional repressor.

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Section: Discussionsupporting

confidence: 62%

Chrysanthemum WRKY gene CmWRKY17 negatively regulates salt stress tolerance in transgenic chrysanthemum and Arabidopsis plants

Song

Gao

et al. 2015

Plant Cell Rep

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“…Here, five major transcription factor families were targeted in the transcriptomic analysis, namely MYB, MADS, AP2/ERF, bHLH, WRKY and TCP (Table S3). Four MYB2-like genes have been described (all homologous to AtMYB44) which negatively regulate flowering in chrysanthemum [35]. Here, the comparison between the WT and M transcriptomes identified a MYB2 transcript which was more strongly transcribed under LD in M than in WT, whereas under SD its abundance was comparable in the two genotypes.…”

Section: Transcription Factors Involved In Floral Induction In Chrysamentioning

confidence: 80%

Gibberellic Acid Signaling Is Required to Induce Flowering of Chrysanthemum Grown under Both Short and Long Days

Dong¹,

Deng²,

Wang³

et al. 2017

Preprint

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Abstract:Flower bud formation and flowering in chrysanthemum occur under short day conditions (SD), but the molecular basis for the switch to reproductive growth is less well understood than in model plants. Here, a spontaneous mutant able to flower under long days is described. In an attempt to reveal the pathway(s) involved in the formation of flower buds under contrasting daylengths, transcriptome sequencing was carried out in plants grown both under SD and under long day conditions (LD). A number of differentially transcribed genes involved in the various known flowering pathways were identified. Both circadian clock genes and CmFTL3 were up-regulated under SD, thereby inducing floral bud formation and flowering. The gibberellin (GA) signalling pathway-related genes GA20ox and GID1 were up-regulated in the mutant under LD, while the catabolic gene GA2ox and GAI was down-regulated, thereby inducing the transcription of CmFTL1, SOC1 and LFY. The GA content of the leaf was higher in the mutant than in the wild type under LD. When treated with GA, the mutant flowered earlier under both SD and LD, but there was no other detectable phenotype difference between the two lines. The indication was that the photoperiod pathway majorly regulates flower bud formation and flowering time in chrysanthemum under SD. GA signalling pathway only plays a subsidiary role for flowering. However, the GA signalling pathway predominated for flowering under LD.

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“…Relatively high expression levels of MYB2 [48] and WRKY [49][50] [49], WRKY12 and WRKY13 [50][51][52] have shown opposite functions in floral regulation. In this work, the MYB and WRKY TFs in SC responded much more strongly to ethephon application than did those in CP; however, additional investigations are needed to verify whether this result is related to the incompletion of the floral transition in SC.…”

Section: More Tfs Responded To Ethephon Stimulation In Sc After Ethepmentioning

confidence: 99%

Comparative Analyses of the Transcriptome and Proteome of Comte de Paris and Smooth Cayenne to Improve the Understanding of Ethephon-Induced Floral Transition in Pineapple

Liu¹,

Liu²,

Shao³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Ethylene is usually used to induce floral transition in pineapple. However, its successful induction in plants categorized as Cayenne is difficult or completely ineffective, and information concerned is limited. The present study was undertaken to investigate the molecular mechanisms underlying this obstacle. Methods: Transcriptome and proteome comparative analyses were performed to explore the important regulation and pathway variations after ethephon induction in the induction-easy ‘Comte de Paris’ (CP) and induction-hard ‘Smooth Cayenne’ (SC) cultivars via RNA-seq (RNA-sequencing) and iTRAQ (isobaric tags for relative and absolute quantification). Results: CP and SC exhibited basic differences at the transcriptomic and proteomic levels before ethephon treatment, including the expression of genes and proteins related to ethylene signal transduction. After ethephon induction, the expression of genes and proteins involved in plant ethylene signal transduction and carbohydrate metabolism responded more strongly in CP than in SC. The expression of the floral meristem identity (FMI) genes AG, TFL and FT exhibited greater changes in CP, and more transcription factors responded in SC. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that many differentially expressed genes (DEGs) in CP were annotated to terms and pathways involved in photoperiodism and shared components involved in carbohydrate metabolism and plant hormone signal transduction. Conclusion: These findings contribute to the understanding of the molecular mechanism underlying the variation between CP and SC in response to ethephon-mediated floral induction.

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Heterologous Expression of the Chrysanthemum R2R3-MYB Transcription Factor CmMYB2 Enhances Drought and Salinity Tolerance, Increases Hypersensitivity to ABA and Delays Flowering in Arabidopsis thaliana

Cited by 101 publications

References 68 publications

Chrysanthemum WRKY gene CmWRKY17 negatively regulates salt stress tolerance in transgenic chrysanthemum and Arabidopsis plants

Chrysanthemum WRKY gene CmWRKY17 negatively regulates salt stress tolerance in transgenic chrysanthemum and Arabidopsis plants

Gibberellic Acid Signaling Is Required to Induce Flowering of Chrysanthemum Grown under Both Short and Long Days

Comparative Analyses of the Transcriptome and Proteome of Comte de Paris and Smooth Cayenne to Improve the Understanding of Ethephon-Induced Floral Transition in Pineapple

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