Heterologous <i>In Vitro</i> Fertility Evaluation of Cryopreserved Iberian Red Deer Epididymal Spermatozoa with Zona‐intact Sheep Oocytes and its Relationship with the Characteristics of Thawed Spermatozoa

Soler, Ana Josefa; Poulin, Noëlle; Fernandez-Santos, M.R.; Cognie, Y.; Esteso, M.C.; Garde, José Julián; Mermillod, Pascal

doi:10.1111/j.1439-0531.2007.00893.x

Cited by 17 publications

(13 citation statements)

References 22 publications

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“…The egg ZP apparently represents a significant barrier to many, if not most, heterospecific crosses in vitro [56]. However, cat oocytes were fertilized by tiger spermatozoa [57]; bovine oocytes were fertilized by goat, ram, and antelope spermatozoa [58][59][60]); and sheep oocytes were fertilized by deer spermatozoa [61]. When comparing heterologous IVF between species, the success of sperm penetration through the ZP is highly variable.…”

Section: Discussionmentioning

confidence: 97%

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

Mugnier

Dell’Aquila

Peláez

et al. 2009

Biology of Reproduction

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The mechanism of fertilization remains largely enigmatic in mammals. Most studies exploring the molecular mechanism underlying fertilization have been restricted to a single species, generally the mouse, without a comparative approach. However, the identification of divergences between species could allow us to highlight key components in the mechanism of fertilization. In the pig, in vitro fertilization (IVF) and polyspermy rates are high, and spermatozoa penetrate easily through the zona pellucida (ZP). In contrast, IVF rates are low in the horse, and polyspermy is scarce. Our objective was to develop a comparative strategy between these two divergent models. First, we compared the role of equine and porcine gametes in the following five functions using intraspecific and interspecific IVF: ZP binding, acrosome reaction, penetration through the ZP, gamete fusion, and pronucleus formation. Under in vitro conditions, we showed that the ZP is a determining element in sperm-ZP attachment and penetration, whereas the capacity of the spermatozoa is of less importance. In contrast, the capacity of the spermatozoa is a key component of the acrosome reaction step. Second, we compared the composition and structure of the equine and porcine ZP. We observed differences in the number and localization of the ZP glycoproteins and in the mesh-like structure of the ZP between equine and porcine species. These differences might correlate with the differences in spermatozoal attachment and penetration rates. In conclusion, our comparative approach allows us to identify determining elements in the mechanism of fertilization.

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Section: Discussionmentioning

confidence: 97%

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

Mugnier

Dell’Aquila

Peláez

et al. 2009

Biology of Reproduction

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“…Previous 475 studies have described cross species fertilization of zona intact oocytes between phylogenetically related species, such as domestic and endangered felids [4,[41][42][43], or between bovine and equine [44], as well as between bovine and artiodactyls [5][6][7][8]45]. …”

Section: Discussionmentioning

confidence: 99%

“…Due to the specificity of the zona pellucida, heterologous IVF application is generally limited to phylogenetically closely related species. Heterologous IVF has been successful when co-incubating oocytes and 55 spermatozoa from cat and tiger spermatozoa [4], bovine and goat, ram or antelope [5][6][7] and sheep and deer respectively [8]. Sometimes, it has been necessary to remove the ZP to achieve heterologous IVF as in the case of dolphin spermatozoa and hamster oocytes [9].…”

Section: Introductionmentioning

confidence: 99%

Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa

et al. 2015

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20Assisted reproductive technologies (ARTs) are of great importance for increasing the genetic diversity in captive animals. The use of bovine or murine oocytes in heterologous IVF provide advantages compared to homologous IVF in non-domestic animals, such as the accessibility to oocytes and the availability of well-developed in vitro maturation systems. The aim of this study was to determine the heterologous IVF 25 parameters using cryopreserved dolphin spermatozoa and zona-intact bovine or murine oocytes, as well as to examine the nuclear chromatin status of the dolphin spermatozoa.All the processes involved in the fertilization including embryo cleavage were observed by confocal microscopy and hybrid embryo formation was confirmed by PCR.Heterologous bovine IVF showed no polyspermy, lower percentages of pronuclear 30 formation and a lower cleavage rate compared to homologous IVF group (34.8 vs.89.3%). Heterologous murine IVF showed a lower cleavage rate than homologous IVF (9.6 vs. 77.1%). With respect to dolphin sperm chromatin, it was more stable, i.e. more resistant to EDTA-SDS decondensation than the bovine sperm chromatin. This study revealed the stability of the dolphin sperm chromatin and the ability of the dolphin 35 spermatozoa to penetrate zona-intact bovine and murine oocytes, leading to hybrid embryo formation.

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“…Therefore, sperm ‘freezability’ (comparison of the pre‐freezing and post‐thawing quality) might not suffice when characterizing samples from a male in the laboratory, being necessary to test the real resistance of spermatozoa by challenging them in physiological and non‐physiological conditions (Roth et al. 1999; Soler et al. 2008).…”

Section: Discussionmentioning

confidence: 99%

“…This example not only shows the impact of between-male differences, but also that the initial quality of a sample (just after thawing, in this case) might be not informative of its real potentiality. Therefore, sperm 'freezability' (comparison of the pre-freezing and post-thawing quality) might not suffice when characterizing samples from a male in the laboratory, being necessary to test the real resistance of spermatozoa by challenging them in physiological and non-physiological conditions (Roth et al 1999;Soler et al 2008). Furthermore, molecular techniques may be used to predict the performance of spermatozoa beyond cryopreservation (Thurston et al 2002;Grunewald et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Response of Thawed Epidi dymal Red Deer Spermatozoa to Increasing Concentrations of Hydrogen Peroxide, and Importance of Individual Male Variability

Domínguez-Rebolledo¹,

Martínez‐Pastor²,

Bisbal³

et al. 2011

Reprod Domestic Animals

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Oxidative stress represents a challenge during sperm manipulation. We have tested the effect of increasing hydrogen peroxide (H(2)O(2)) levels on red deer spermatozoa after cryopreservation, and the role of male-to-male variation in that response. In a first experiment, eight thawed samples were submitted to 0, 25, 50, 100 and 200 μm H(2)O(2) for 2 h at 37 °C. Intracellular reactive oxygen species (H(2)DCFDA-CM) increased with H(2)O(2) concentration, but we only detected a decrease in sperm function (motility by CASA and chromatin damage by sperm chromatin structure assay) with 200 μm. Lipoperoxidation assessed by the thiobarbituric acid reactive substance (TBARS) method increased slightly with 50 μm H(2)O(2) and above. In a second experiment, samples from seven males were submitted to 0 and 200 μm H(2)O(2) for 2 h, triplicating the experiment within each male. Males differed at thawing and regarding their response to incubation and H(2)O(2) presence. We found that the kinematic parameters reflected male-to-male variability, whereas the response of the different males was similar for lipid peroxidation and viability. A multiparametric analysis showed that males grouped differently if samples were assessed after thawing, after incubation without H(2)O(2) or after incubation with H(2)O(2) . Red deer spermatozoa are relatively resilient to H(2)O(2) after thawing, but it seems to be a great male-to-male variability regarding the response to oxidative stress. The acknowledgement of this individual variability might improve the development of optimized sperm work protocols.

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Heterologous In Vitro Fertility Evaluation of Cryopreserved Iberian Red Deer Epididymal Spermatozoa with Zona‐intact Sheep Oocytes and its Relationship with the Characteristics of Thawed Spermatozoa

Cited by 17 publications

References 22 publications

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa

Response of Thawed Epidi dymal Red Deer Spermatozoa to Increasing Concentrations of Hydrogen Peroxide, and Importance of Individual Male Variability

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