Heterologous immunoassay for screening macrolide antibiotics residues in milk based on the monoclonal antibody of tylosin

Zhang, Jian Kang; You, Qi; Liu, Juxiang; Wang, Jian Ping

doi:10.1080/09540105.2012.705820

Cited by 13 publications

(3 citation statements)

References 30 publications

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“…It is well known that antibodies are the core reagents for the development of immunoassay methods. To date, the antibodies used in the simultaneous detection of tylosin and tilmicosin are mainly obtained by immunization with Shiff base arm hapten derived from carbonyl amino condensation and some of these reported immunoassays are also sensitive and accurate [2,[25][26][27][28]. Thus, our first attempt was to use the reported Shiff base hapten strategy, but this failed to obtain an antibody against tylosin and tilmicosin because of the poor and unstable immune response.…”

Section: Introductionmentioning

confidence: 99%

Design of Novel Haptens and Development of Monoclonal Antibody-Based Immunoassays for the Simultaneous Detection of Tylosin and Tilmicosin in Milk and Water Samples

et al. 2019

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In this work, a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (icELISA) was established to detect tylosin and tilmicosin in milk and water samples. A sensitive and specific monoclonal antibody was prepared by rational designed hapten, which was achieved by directly oxidizing the aldehyde group on the side chain of tylosin to the carboxyl group. Under the optimized conditions, the linear range of icELISA for tylosin and tilmicosin were 1.3 to 17.7 ng/mL and 2.0 to 47.4 ng/mL, with half-maximal inhibition concentration (IC50) values of 4.7 and 9.6 ng/mL, respectively. The cross-reactivity with other analogues of icELISA was less than 0.1%. The average recoveries of icELISA for tylosin and tilmicosin ranged from 76.4% to 109.5% in milk and water samples. Besides, the detection results of icELISA showed good correlations with HPLC-MS/MS. The proposed icELISA was satisfied for rapid and specific screening of tylosin and tilmicosin residues in milk and water samples.

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Section: Introductionmentioning

confidence: 99%

Design of Novel Haptens and Development of Monoclonal Antibody-Based Immunoassays for the Simultaneous Detection of Tylosin and Tilmicosin in Milk and Water Samples

et al. 2019

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“…Macrolides are constituted by a macrocyclic lactone formed by 14, 15 or 16 carbon atoms linked to sugar molecules, through glycosidic bonds , which display highly active against Gram‐positive bacteria and mycoplasma species . Due to their high oral bioavailability and their ability to accumulate intracellularly in many tissues and organs , they are widely used in medical and veterinary practices to prevent and treat diseases in swine, poultry, cattle, and sheep.…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic‐assisted dispersive liquid–liquid microextraction based on a simple and green deep eutectic solvent for preconcentration of macrolides from swine urine samples

Liao

Wen

Guang

et al. 2019

Separation Science Plus

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In this study, a simple and green deep eutectic solvent based dispersive liquidliquid microextraction methodology had been proposed for the assessment of four macrolides in swine urine samples. Some important parameters including type and volume of extraction solvent and demulsifier solvent, sample pH, salt concentration affecting extraction efficiency were investigated. The quantitative recoveries were achieved at pH 9 and 20% (w/v) NaCl by using 0.3 mL of deep eutectic solvent consisting of choline chloride-phenol (1:2, molar ratio) and 0.3 mL of tetrahydrofuran. Under optimal conditions, the linear ranges of four macrolides were 0.5-20.0 μg/L with correlation coefficient higher than 0.999. Limits of detection and quantification were in the ranges of 0.02-0.1 and 0.1-0.5 μg/L, respectively. The relative recoveries for macrolides in spiked swine urine samples ranged from 80 to 93% with relative standard deviations less than 6%. K E Y W O R D Sdeep eutectic solvents, dispersive liquid-liquid microextraction, macrolides, urine samples 22

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“…However, most of these assays are time consuming and require expensive equipment, specialized technical personnel, and complicated sample pre-treatment and, thus, are unsuitable for extensive screening and field detection. Meanwhile, several researchers have published studies on ELISA and immunochromatographic assay for detecting TYL and TIM simultaneously, which have the advantages of low cost, high sensitivity, high throughput, and ease of operation Zhang, 2013;Zhang, Liu, Wang, Chai, & Wang, 2012).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of tylosin and tilmicosin in honey using a novel immunoassay and immunochromatographic strip based on an innovative hapten

Song

Liu

et al. 2015

Food and Agricultural Immunology

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Tylosin (TYL) and tilmicosin (TIM) are macrolide antibiotics, and maximum residue limits (MRLs) have been set to control their illegal usage in food. We developed a sensitive indirect competitive enzyme-linked immunosorbent assay and rapid immunochromatographic strip (ICS) test to simultaneously detect TYL and TIM based on an innovative hapten (TYLcarboxymethoxylamine hemihydrochloride). The monoclonal antibody 2B3 was obtained with the isotype IgG1, and TYL and TIM had half maximal inhibitory concentrations of 0.57 and 2.10 ng/ml, respectively. The cross-reactivity values were 100%, 27.57%, 97.43%, and 62.42% for TYL, TIM, desmycosin, and acetylisovaleryl tylosin, respectively. By optimizing, the standard dilution buffer was based on 0.8% NaCl, pH 7.4 and 5% acetonitrile. The recoveries ranged from 89.37% to 112.00% in the honey samples, which suggests that the assay would be reliable in the analysis of real honey samples. In addition, an ICS was developed for qualitative, semi-quantitative, and spot detection analysis, with cutoff values of 5 and 15 ng/ml for TYL and TIM in phosphatebuffered saline, and 10 and 20 ng/ml for TYL and TIM in honey, respectively. The results show that these assays can be used to analyze real samples with the strictest MRL being 50 ng/ml for both TYL and TIM.

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Heterologous immunoassay for screening macrolide antibiotics residues in milk based on the monoclonal antibody of tylosin

Cited by 13 publications

References 30 publications

Design of Novel Haptens and Development of Monoclonal Antibody-Based Immunoassays for the Simultaneous Detection of Tylosin and Tilmicosin in Milk and Water Samples

Design of Novel Haptens and Development of Monoclonal Antibody-Based Immunoassays for the Simultaneous Detection of Tylosin and Tilmicosin in Milk and Water Samples

Ultrasonic‐assisted dispersive liquid–liquid microextraction based on a simple and green deep eutectic solvent for preconcentration of macrolides from swine urine samples

Simultaneous detection of tylosin and tilmicosin in honey using a novel immunoassay and immunochromatographic strip based on an innovative hapten

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