1998
DOI: 10.1095/biolreprod58.2.475
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Heterologous In Vitro Fertilization and Sperm Capacitation in an Endangered African Antelope, the Scimitar-Horned Oryx (Oryx dammah)1

Abstract: Scimitar-horned oryx sperm function was studied using protocols developed for domestic cattle. Objectives were to assess sperm 1) viability and motility in vitro over time, 2) capacitation in heparin- or calcium-supplemented medium, and 3) function in an in vitro fertilization system using heterologous (domestic cow) oocytes. Seminal aliquots were washed, and sperm were resuspended in 1) Talp with 5% fetal calf serum (TALP), 2) TALP + 10 microM heparin, 3) TALP + 20 microM heparin, and 4) TALP + 10 mM CaCl. At… Show more

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Cited by 94 publications
(67 citation statements)
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“…However, in this study, the rate of fertilization as assessed by the formation of pronuclei did not differ when yak and cattle oocytes were inseminated with either yak or cattle spermatozoa. These results are in agreement with those reported in previous studies showing that cattle oocytes can be fertilized with sperm from the oryx [19,20], yak [9,11], bison, banteng and gaur [9], suggesting little cross-species specificity with regard to gamete interaction among Bovidae. Thus, penetration failure and/or abnormal fertilization are not the main factors that contribute to poor conception rates when female yaks are crossed with cattle.…”
Section: Discussionsupporting
confidence: 93%
“…However, in this study, the rate of fertilization as assessed by the formation of pronuclei did not differ when yak and cattle oocytes were inseminated with either yak or cattle spermatozoa. These results are in agreement with those reported in previous studies showing that cattle oocytes can be fertilized with sperm from the oryx [19,20], yak [9,11], bison, banteng and gaur [9], suggesting little cross-species specificity with regard to gamete interaction among Bovidae. Thus, penetration failure and/or abnormal fertilization are not the main factors that contribute to poor conception rates when female yaks are crossed with cattle.…”
Section: Discussionsupporting
confidence: 93%
“…Para avaliação da integridade do acrossoma, utilizou-se o corante isotiocianato de fluoresceína conjugado a Peanut agglutinin (FITC-PNA; Sigma-Aldrich®, St Louis, MO, USA), de acordo com a técnica descrita por Roth et al (1998), em que uma alíquota de 20μL da solução estoque de FITC-PNA (1mg/mL) foi descongelada e adicionada a 480μL de PBS para obter a concentração final de 100μg/mL. Alíquotas (20μL) desta solução foram colocadas sobre esfregaços de lâminas, as quais foram incubadas por 20 minutos em câmara úmida a 4°C, na ausência de luz.…”
Section: Methodsunclassified
“…We therefore used heterologous in vitro fertilization (IVF) with zona-free in vitro matured bovine oocytes in order to assess the fertilizing ability and the developmental potential of cryopreserved semen from different stags. In vitro capacitation of the Oryx sperm (Oryx dammah) [58] and fertilizing ability of different spermatozoa of the genus Bos [46] have been assessed by heterologous IVF with bovine oocytes. The oocyte penetration assay is also used for testing sperm in canids [26].…”
Section: Few Individuals Are Available and Sometimes In Widely Disparmentioning
confidence: 99%