Heterologous overexpression of Pseudomonas umsongensis halohydrin dehalogenase in Escherichia coli and its application in epoxide asymmetric ring opening reactions

Xue, Feng; Ya, Xiangju; Tong, Qi; Xiu, Yuansong; Huang, He

doi:10.1016/j.procbio.2018.09.018

Cited by 26 publications

(10 citation statements)

References 33 publications

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“…respond to the substance pressure in soil by activating Resistance-Nodulation-Division (RND) efflux pumps which remove phenols from the cell. They also have a gene (HHDH) encoding halohydrin dehalogenase, which opens the epoxide ring [79]. Moreover, a number of metabolic pathways were identified, which are induced by Pseudomonas sp.…”

Section: Counts and Diversity Of Bacteriamentioning

confidence: 99%

Soil Microbiome Response to Contamination with Bisphenol A, Bisphenol F and Bisphenol S

Zaborowska

Wyszkowska

Borowik

2020

IJMS

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The choice of the study objective was affected by numerous controversies and concerns around bisphenol F (BPF) and bisphenol S (BPS)—analogues of bisphenol A (BPA). The study focused on the determination and comparison of the scale of the BPA, BPF, and BPS impact on the soil microbiome and its enzymatic activity. The following parameters were determined in soil uncontaminated and contaminated with BPA, BPF, and BPS: the count of eleven groups of microorganisms, colony development (CD) index, microorganism ecophysiological diversity (EP) index, genetic diversity of bacteria and activity of dehydrogenases (Deh), urease (Ure), catalase (Cat), acid phosphatase (Pac), alkaline phosphatase (Pal), arylsulphatase (Aryl) and β-glucosidase (Glu). Bisphenols A, S and F significantly disrupted the soil homeostasis. BPF is regarded as the most toxic, followed by BPS and BPA. BPF and BPS reduced the abundance of Proteobacteria and Acidobacteria and increased that of Actinobacteria. Unique types of bacteria were identified as well as the characteristics of each bisphenol: Lysobacter, Steroidobacter, Variovorax, Mycoplana, for BPA, Caldilinea, Arthrobacter, Cellulosimicrobium and Promicromonospora for BPF and Dactylosporangium Geodermatophilus, Sphingopyxis for BPS. Considering the strength of a negative impact of bisphenols on the soil biochemical activity, they can be arranged as follows: BPS > BPF > BPA. Urease and arylsulphatase proved to be the most susceptible and dehydrogenases the least susceptible to bisphenols pressure, regardless of the study duration.

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Section: Counts and Diversity Of Bacteriamentioning

confidence: 99%

Soil Microbiome Response to Contamination with Bisphenol A, Bisphenol F and Bisphenol S

Zaborowska

Wyszkowska

Borowik

2020

IJMS

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“…According to Furmańczyk et al (2017), Pseudomonas umsongensis has genes rfbADB and rfbC which encode proteins responsible for the synthesis of rhamnolipid, which is a biosurfactant effective in hydrocarbon degradation and which facilitates their emulsification. It also produces a gene HHDH-Pu which encodes halohydrin dehalogenase, which is responsible for enzymatic opening of the epoxide ring (Xue et al 2018). The aerobic catabolism of bisphenols is catalysed by monooxygenases, phenol hydroxylase, which adds one atom from an oxygen molecule to an aromatic ring, and toluene/oxylen monooxygenase (TOMO), capable of hydroxylation of more than one position of the aromatic ring (Cafaro et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Role of Chlorella sp. and rhamnolipid 90 in maintaining homeostasis in soil contaminated with bisphenol A

2020

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Purpose The knowledge about the impact of BPA on soil health does not correspond to the great interest in its analogues. Therefore, a study was conducted to determine the potentially negative impact of BPA on the biochemical properties of soil. The study also evaluated the effectiveness of two biostimulants in eliminating potential homeostasis disorders caused by BPA. Materials and methods A pot experiment was conducted under supervised conditions. BPA at five contamination levels was added to the soil of the granulometric composition of sandy loam at 0, 0.1, 2, 40 and 800 mg BPA kg −1 of a dry matter (DM) of soil. The biochemical activity of the soil was interpreted through the activity of dehydrogenases (Deh), urease (Ure), catalase (Cat), acid phosphatase (Pac), alkaline phosphatase (Pal), arylsulphatase (Aryl) and β-glucosidase (Glu) whose activity was determined on days 5, 15 and 45 of the study. The biostimulative potential of Chlorella sp. and rhamnolipid 90 (which eliminates the undesirable effects of BPA on the parameters) was expressed with IF B-the factor of the impact of increasing of bisphenol (BP) soil contamination levels. The response of spring barley to increasing BPA pressure was analysed with the plant resistance index (RS). The study was made more comprehensive by determination of the macronutrient content in the plants. Results and discussion The sensitivity of individual enzymes to increasing bisphenol pressure on the 45th day of the experiment can be arranged in the following sequence: Deh > Ure > Glu > Pac > Cat > Aryl > Pal. Biostimulation of soil with Chlorella sp. gave better results than with rhamnolipid 90. A compilation of BPA 800 mg BPA kg −1 DM of soil and Chlorella sp. brought about an increase in the activity of Glu on the 45th day of the experiment and Pac, Pal and Aryl on the 5th day. Only at this contamination level did BPA stimulate the crop growth in all the parallel plots except in those biostimulated by Chlorella sp. Only algae significantly reduced the negative BPA impact on the N, Ca and K content in spring barley. Conclusions The experiment emphasised the significant inhibitory impact of BPA on the biochemical activity of soil which, in consequence, upset the microbial balance of soil processes. Chlorella sp. played a more important role in maintaining the soil homeostasis than rhamnolipid 90, which did not correspond to its negative impact on the yield of spring barley.

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“…e gene of Rhizobium sp. encoding haloalkane dehydrogenase [68] and halohydrin dehydrogenase (HHDH) producing strain Pseudomonas umsongensis YCIT1612 [66], both expressed in E. coli, also help in bioremediation by process of degradation of haloalkane and performing epoxide assimilating reaction, respectively, as shown in Table 3.…”

Section: Laccasesmentioning

confidence: 99%

Microbial Enzymes Used in Bioremediation

Bhandari

Poudel

Marahatha

et al. 2021

Journal of Chemistry

172

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Emerging pollutants in nature are linked to various acute and chronic detriments in biotic components and subsequently deteriorate the ecosystem with serious hazards. Conventional methods for removing pollutants are not efficient; instead, they end up with the formation of secondary pollutants. Significant destructive impacts of pollutants are perinatal disorders, mortality, respiratory disorders, allergy, cancer, cardiovascular and mental disorders, and other harmful effects. The pollutant substrate can recognize different microbial enzymes at optimum conditions (temperature/pH/contact time/concentration) to efficiently transform them into other rather unharmful products. The most representative enzymes involved in bioremediation include cytochrome P450s, laccases, hydrolases, dehalogenases, dehydrogenases, proteases, and lipases, which have shown promising potential degradation of polymers, aromatic hydrocarbons, halogenated compounds, dyes, detergents, agrochemical compounds, etc. Such bioremediation is favored by various mechanisms such as oxidation, reduction, elimination, and ring-opening. The significant degradation of pollutants can be upgraded utilizing genetically engineered microorganisms that produce many recombinant enzymes through eco-friendly new technology. So far, few microbial enzymes have been exploited, and vast microbial diversity is still unexplored. This review would also be useful for further research to enhance the efficiency of degradation of xenobiotic pollutants, including agrochemical, microplastic, polyhalogenated compounds, and other hydrocarbons.

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Heterologous overexpression of Pseudomonas umsongensis halohydrin dehalogenase in Escherichia coli and its application in epoxide asymmetric ring opening reactions

Cited by 26 publications

References 33 publications

Soil Microbiome Response to Contamination with Bisphenol A, Bisphenol F and Bisphenol S

Soil Microbiome Response to Contamination with Bisphenol A, Bisphenol F and Bisphenol S

Role of Chlorella sp. and rhamnolipid 90 in maintaining homeostasis in soil contaminated with bisphenol A

Microbial Enzymes Used in Bioremediation

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