Heterologous strategy enhancing the sensitivity of the fluorescence polarization immunoassay of clinafloxacin in goat milk

Chen, Jiahong; Shanin, I. A.; Lv, Shuwei; Wang, Qiang; Mao, Chuanbin; Xu, Zhen-Lin; Sun, Yuan; Wu, Qing; Eremin, Sergei A.; Lei, Hongtao

doi:10.1002/jsfa.7228

Cited by 21 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another assay has been established a homogenous FPIA based on the monoclonal antibody and the tracer for the simultaneous determination of cefalexin and cefadroxil in milk samples (Zhang, Wang, Mi, Wenren, & Wen, 2014). A heterologous structure of FPIA was established for the determination of clinafloxacin in goat milk which has been observed to be more sensitive than homologous structure (Chen, Shanin, et al, 2016). Another sensitive quantitative FPIA immunochemical approach has been reported for the screening of gentamicin in milk samples.…”

Section: Fluorescence Immunoassay/fluorescence Polarization Immunoassaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

132

View full text Add to dashboard Cite

Food contamination with antibiotic residues has become a worldwide problem due to the heavy and improper use of antibiotics. The development of fast, high-throughput, easy, and economical screening methods for the detection of antibiotic residues is an important requirement as an alternative to the traditional assays. Immunoassays are widespread promising methods that can achieve the conditions of an analytical method in the evaluation of food and environmental protection. This review offers an overview of the preparation of antibodies and the currently established immuneassays for the antibiotic residues detection, including enzymelinked immunosorbent assay, fluorescence immunoassay, radioimmunoassay, colloidal gold immunoassay, and chemiluminescence immunoassay. Furthermore, it will elaborate on the future perspective on the performance and improvement of these assays for the determination of antibiotic residues in food samples. These assays include a simple sample preparation and high-sensitive detection that offer a significant assurance for the screening of antibiotic residues.

show abstract

Section: Fluorescence Immunoassay/fluorescence Polarization Immunoassaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

132

View full text Add to dashboard Cite

show abstract

“…With the heterologous tracer 5-OH-DCF-AMF, approximately the same sensitivity as with the homologous tracer DCF-Ahx-AMF was obtained (compare Table 2). According to some studies [27][28][29], heterologous tracers may improve the sensitivity.…”

Section: Sensitivity and Measurement Rangementioning

confidence: 99%

Fluorescence polarization immunoassay for the determination of diclofenac in wastewater

et al. 2020

View full text Add to dashboard Cite

Pharmacologically active compounds are often detected in wastewater and surface waters. The nonsteroidal anti-inflammatory drug diclofenac (DCF) was included in the European watch list of substances that requires its environmental monitoring in the member states. DCF may harmfully influence the ecosystem already at concentrations ≤ 1 μg L−1. The fast and easy quantification of DCF is becoming a subject of global importance. Fluorescence polarization immunoassay (FPIA) is a homogeneous mix-and-read method which does not require the immobilization of reagents. FPIA can be performed in one phase within 20–30 min, making it possible to analyse wastewater without any complicated pre-treatment. In this study, new tracer molecules with different structures, linking fluorophores to derivatives of the analyte, were synthesized, three homologous tracers based on DCF, two including a C6 spacer, and one heterologous tracer derived from 5-hydroxy-DCF. The tracer molecules were thoroughly assessed for performance. Regarding sensitivity of the FPIA, the lowest limit of detection reached was 2.0 μg L−1 with a working range up to 870 μg L−1. The method was validated for real wastewater samples against LC-MS/MS as reference method with good agreement of both methods.

show abstract

“…Some studies have been reported for the detection of FQs by FPIA [19][20][21]. In our previous study, heterologous tracers were recruited to establish FPIA for the detection of six FQs based on anti-clinfloxacin polyclonal antibody (pAb) [22]. However, since enrofloxacin is more popular than other FQs [15], specific immunoassays are needed to accurately quantify enrofloxacin residues.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Polarization Immunoassay for Determination of Enrofloxacin in Pork Liver and Chicken

Shen

Chen

et al. 2019

Molecules

Self Cite

View full text Add to dashboard Cite

Enrofloxacin (ENR) is a widely used fluoroquinolone (FQ) antibiotic for antibacterial treatment of edible animal. In this study, a rapid and highly specific fluorescence polarization immunoassay (FPIA) was developed for monitoring ENR residues in animal foods. First, ENR was covalently coupled to bovine serum albumin (BSA) to produce specific polyclonal antibodies (pAbs). Three fluorescein-labeled ENR tracers (A, B, and C) with different spacers were synthesized and compared to obtain higher sensitivity. Tracer C with the longest arm showed the best sensitivity among the three tracers. The developed FPIA method showed an IC50 (50% inhibitory concentration) of 21.49 ng·mL−1 with a dynamic working range (IC20–IC80) of 4.30–107.46 ng·mL−1 and a limit of detection (LOD, IC10) of 1.68 ng·mL−1. The cross-reactivity (CR) of several structurally related compounds was less than 2%. The recoveries of spiked pork liver and chicken samples varied from 91.3% to 112.9%, and the average coefficients of variation were less than 3.83% and 5.13%, respectively. The immunoassay took only 8 min excluding sample pretreatment. This indicated that the established method had high sensitivity, specificity, and the advantages of simplicity. Therefore, the proposed FPIA provided a useful screening method for the rapid detection of ENR residues in pork liver and chicken.

show abstract

Heterologous strategy enhancing the sensitivity of the fluorescence polarization immunoassay of clinafloxacin in goat milk

Abstract: This proposed heterologous strategy for enhanced FPIA is sensitive and rapid enough for the high-throughput detection of clinafloxacin residue in goat milk.

Cited by 21 publications

References 30 publications

Current advances in immunoassays for the detection of antibiotics residues: a review

Current advances in immunoassays for the detection of antibiotics residues: a review

Fluorescence polarization immunoassay for the determination of diclofenac in wastewater

Fluorescence Polarization Immunoassay for Determination of Enrofloxacin in Pork Liver and Chicken

Contact Info

Product

Resources

About