Heterophile Antigen Detected by Infectious Mononucleosis Sera on Lymphoid Cells

The multiple nature of heterophile, Paul-Bunnell (P-B) antigen has been suggested by our previous studies as well as those of others. In the present study, two distinct antigens of P-B specificity were defined by means of hemagglutination and immunodiffusion tests in agarose gel; one antigen (BS) was shared by bovine (BRBC) and sheep red blood cells (SRBC) and another antigen (B) was limited to BRBC. Both anti-BS and anti-B antibodies were shown to be present in sera of 106 patients with infectious mononucleosis (IM) whereas they were virtually absent from the sera of the vast majority of patients with diseases other than IM and normal sera. Absorption and agglutination inhibition tests demonstrated the presence of BS and B antigens on horse erythrocytes. Goat erythrocytes were also shown to possess BS antigen, however, B antigen was found on erythrocytes of some but not all individual goats. By means of the previously established procedure, BS antigen was extracted from stromata of BRBC, SRBC and erythrocytes of horse and goat, and B antigen from BRBC and erythrocytes of some goats. BS and B antigens were also extracted from bovine lymphocytes and murine thymus and spleen tissues but not murine erythrocytes.

Section: Discussionmentioning

confidence: 99%

Studies on Paul-Bunnell (P-B) Antigen-Antibody System

Masaki¹,

Kano²,

Merrick³

et al. 1981

“…This was performed as a test for hemolysis in agar gel following the previously described procedure [2,4]. In brief, one volume of a 1.25-percent suspension of BRBC (v/v) was mixed at 43 °C with six volumes of 1% Difco Bacto agar containing DEAE dextran at a concentration of 0.5°/o (w/v).…”

Section: Methodsmentioning

confidence: 99%

“…Absorption studies brought clear-cut evidence that the observed results were due to the reactions of heterophile antibodies in IM sera with the heterophile an tigen present on malignant murine lymphoid cells which in all instances were of T cell origin. Further studies showed that normal murine thymo-cytes and splenocytes but not other cells contain the heterophile antigen [4], Comparative absorption experiments indicated that the heterophile antigen on normal murine lymphoid cells has lower density and/or ap pears in less complete form than on malignant lymphoid cells [4].…”

Section: Introductionmentioning

confidence: 99%

Studies on Heterophile Antigen in Lymphoma and Leukemia Spleens by Means of Absorption of Infectious Mononucleosis Sera

Milgrom¹,

Kano²,

Fjelde³

1973

Spleen specimens from patients suffering from lymphomas and leukemias were studied for the presence of heterophile antigen by means of absorption of infectious mononucleosis sera. In the lymphoma group, 11 of 16 spleens tested diminished significantly the titer of lysins for bovine red blood cells in infectious mononucleosis sera. The absorbing effect of these spleen specimens correlated well with the replacement of the normal organ structure by malignant cells. None of five spleens from leukemia patients had a significant absorbing effect on infectious mononucleosis sera.

“…The absorption efficacy of the bone marrow cells was quite analogous to that of murine thymocytes which were shown to possess P-B antigen in our previous studies [8,9]. Finally, relationship between P-B antigen and Fc receptor sites on mu rine bone marrow cells was studied by means of blocking experiments [6].…”

Section: Resultsmentioning

confidence: 55%

“…It was found that oxen possess the heterophile [Paul-Bunnell (P-B)] antigen not only on erythrocytes but also on all cells thus far examined including cells of kidney [7], aortic intima, adrenal and spleen [5], as well as platelets [14], Of particular interest was the distribution of P-B antigen in murine lymphoid cells: thymocytes as well as Thy-1 positive lymphoma cells have been shown to possess the antigen [8,9], while the antigen could not be detected on spleen cells of nude mice [unpublished data]. It was, there fore, concluded that the P-B antigen is a marker for murine T lympho cytes.…”

Section: Introductionmentioning

confidence: 99%

Heterophile Antigen Detectable by Infectious Mononucleosis Sera on Bone Marrow Cells

Mori¹,

Kano²,

Milgrom³

1976

Self Cite

By means of mixed agglutination test and indirect immunofluorescence test, reactions of infectious mononucleosis sera with bone marrow cells were investigated. Positive reactions were observed with bovine, murine and rat bone marrow cells, but negative results were obtained with cells of monkeys and guinea pigs. Absorption studies demonstrated that the observed reactions are due to interaction of the Paul-Bunnell antigen on bone marrow cells with its corresponding antibodies in infectious mononucleosis sera. Whereas all cells of bovine bone marrow gave positive results, only 50% of murine or rat cells were positive and these cells were shown to be neutrophilic granulocytes. Evidence was also presented that the Paul-Bunnell antigen and Fc receptor are present, apart from each other, on murine neutrophilic granulocytes.