Heteropolydeoxynucleotides synthesized with terminal deoxyribonucleotidyltransferase. II. Nearest neighbor frequencies and extent of digestion by micrococcal deoxyribonuclease

Preparation and properties of the repeating sequence polymer d(A-A-T)n.d(A-T-T)n

“…The samples were cooled and centrifuged, and the supernatants were dialyzed against 0.01 m sodium pyrophosphate and then distilled water to remove unreacted deoxyribonucleoside triphosphates. The polymers were degraded to their 3 'mononucleotides using micrococcal DNase and spleen phosphodiesterase and were separated by electrophoresis on paper (Josse et al, 1961;Ratliff et al, 1968). The radioactive spots were located with a Packard radiochromatogram scanner, cut out, and counted in a Packard Tri-Carb liquid scintillation counter.…”

“…Nearest neighbor analysis was done for each of the polymers according to the procedures of Josse et al (1961) and Ratliff et al (1968). Size distributions were determined as described by Hayes and Mitchell (1969) and by Jang and Bartl (1971).…”

Preparation and properties of the repeating sequence polymers d(A-I-C)n.d(I-C-T) n and d(A-G-C)n.d(G-C-T)n

“…Nearest neighbor analyses of the enzymatically synthesized polymers were done according to the procedures of Josse et al (1961) and Ratliff et al (1968Ratliff et al ( , 1973. The method of following the kinetics of transcription of d(A-I-C)"-d(I-C-T)" by E. coli RNA polymerase was essentially that described by Morgan (1970).…”

Biosynthesis of DNA

“…Polymerization of mixtures of deoxynucleoside triphosphates by termi nal deoxynucleotidyltransferase of calf thymus yields polymers without rec ognizable order in sequence (121)(122)(123)(124). The sequence is, however, influ enced by the identity of the nucleotide substrates as well as the stoichiome try of the mixture.…”