Preparation and properties of the repeating sequence polymer d(A-A-T)n.d(A-T-T)n

Interaction of phenazines with polydeoxyribonucleotides

“…Stock solutions of 10"2 *-10-3 m of all polynucleotides were prepared in 10-3 m Na+ (buffer or NaCl). The RNA polymerase solution contained 5.3 mg/ml with 2630 units/mg (Ratliff et al, 1967). For the inhibition runs, it was suitably diluted with a solution containing 0.01 m Tris (pH 7.9), 0.1 m MgCh, 0.01 m mercaptoethanol, 5 X 10-5 m EDTA, and 1 mg/ml of bovine serum albumin.…”

Preparation and properties of the repeating sequence polymers d(A-I-C)n.d(I-C-T) n and d(A-G-C)n.d(G-C-T)n

“…Initial In our attempts to synthesize the various polydeoxyribonucleotides containing repeating trinucleotide sequences using complementary short-chain oligodeoxyribonucleotides as templates, we have found that the best yields were obtained with high concentrations of deoxyribonucleoside triphosphates (3-5 mM) and low temperature (15°) using either E. coli or M. luteus DNA polymerase. For the synthesis of more of the same DNAs with repeating trinucleotide sequences using previously prepared DNAas as template, the M. luteus DNA polymerase gave better yields of polymer than the E. coli DNA polymerase when the template had 100 base pairs or less (Ratliff et al, 1973). If an attempt was made to employ as template unfractionated polydeoxyribonucleotides which were themselves products of a previous enzymatic synthesis and consequently of high molecular weight, overall yields with either enzyme were less.…”

“…Analytical buoyant density centrifugation was carried out essentially as described previously (Ratliff et al, 1973).…”

“…Nearest neighbor analyses of the enzymatically synthesized polymers were done according to the procedures of Josse et al (1961) and Ratliff et al (1968Ratliff et al ( , 1973. The method of following the kinetics of transcription of d(A-I-C)"-d(I-C-T)" by E. coli RNA polymerase was essentially that described by Morgan (1970).…”

“…Preparation and chemical polymerization of d(Á-G-C) and d(C-T-G) sequences from protected deoxynucleoside 5'monophosphates were modeled on published procedures of Narang et al (1967a,b). Details of the method have been published perviously by Ratliff et al (1973). The protected monomer 7V-anisoyl-C3'-isobutyryldeoxycytidine S'-monophosphate [d-p-AnC(z'Bu)] was prepared by shaking a suspension of pyridinium A-anisoyldeoxycytidine S'-monophosphate in anhydrous pyridine with isobutyric anhydride for 24 hr.…”