Heterosandwich immunoswab assay for dengue virus Ns1 antigen detection

Ganguly, Advaita; Malabadi, Ravindra B.; Loebenberg, Raimer; Suresh, Mavanur R.; Sunwoo, Hoon H.

doi:10.1016/j.diagmicrobio.2013.10.006

Cited by 5 publications

(8 citation statements)

References 21 publications

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“…Vero cells are used to culture Nipah virus (NiV) with observable cytopathic effects in three days . The main tests used are real time polymerase chain reaction (RT-PCR) from bodily fluids and antibody detection via enzyme-linked immunosorbent assay (ELISA) [26][27][28][29][30][31][32][33][34][35][36][37]. ELISA is often followed by a serum neutralisation test or PCR [26][27][28][29][30][31][32][33][34][35][36][37].…”

Section: Nipah Virus (Niv): Diagnosismentioning

confidence: 99%

“…The main tests used are real time polymerase chain reaction (RT-PCR) from bodily fluids and antibody detection via enzyme-linked immunosorbent assay (ELISA) [26][27][28][29][30][31][32][33][34][35][36][37]. ELISA is often followed by a serum neutralisation test or PCR [26][27][28][29][30][31][32][33][34][35][36][37]. Virus isolation and neutralisation methods are also used for diagnosis but are constrained to BSL-4 facilities.…”

Section: Nipah Virus (Niv): Diagnosismentioning

confidence: 99%

“…Virus isolation and neutralisation methods are also used for diagnosis but are constrained to BSL-4 facilities. Other tests used include polymerase chain reaction (PCR) assay, and virus isolation by cell culture [26][27][28][29][30][31][32][33][34][35][36][37]. Next generation sequencing is an alternative method aiding in effective identification of viral strain, however the method is not frequently used in diagnosis and when considering expenses [26][27][28][29][30][31][32][33][34][35][36][37].…”

Section: Nipah Virus (Niv): Diagnosismentioning

confidence: 99%

“…During, Covid-19, the infusion of Cannabis flower with a morning cup of tea has saved the life of many people . Cannabis oil was used as dengue mosquito repellent for controlling dengue viral fever [26][27][28][29][30][31][32][33][34][35][36][37], bacterial infections and fungal diseases [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

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Cannabis sativa: 2023-Outbreak and Re-emergence of Nipah virus (NiV) in India: Role of Hemp oil

Ravindra B. Malabadi,

Kiran P. Kolkar,

Raju K. Chalannavar

et al. 2023

GSC Biol. Pharm. Sci.

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This review paper highlights and updates the outbreak of Nipah virus (NiV), an emerging zoonotic bat borne virus that can cause severe respiratory illness and deadly encephalitis in humans. Nipah virus (NiV) outbreak was first reported in 1998 from Malaysia and then recorded in Bangladesh, India, Philippines, Singapore and Thailand. A recent fifth outbreak of Nipah virus (NiV) during August-September 2023 in Kerala State, India brought this emerging-re-emerging virus into the spotlight again. Due to the lack of vaccines and drugs with proven effectiveness against Nipah virus (NiV), treatment of patients is limited to supportive and prophylactic. Fruit bats are the main reservoir for this virus, which can cause disease in humans and animals. Nipah virus (NiV) can be transmitted from bats or livestock to humans, typically via contaminated food (fruit or raw date palm sap), and person-to-person through respiratory secretions. Hemp seed oil has gained remarkable popularity due to its perceived therapeutic properties, particularly antiviral properties for potential health benefits. During the recent outbreak of Nipah virus (NiV), the local traditional healers in India used hemp oil as the mouth wash for controlling throat infections, head ache, vomiting and suggested for the consumption of hemp seeds and oil as the functional food. However, clinical trials and scientific data supporting antiviral activity of Cannabis oil (CBD oil) against Nipah virus (NiV) is lacking. Therefore, further research should focus on exploring the molecular mechanisms of hemp oil against Nipah virus (NiV) is warranted. Multicenter clinical trials should be performed to validate the efficacy of hemp oil alone or in the form of formulations for the treatment of viral infections.

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Section: Nipah Virus (Niv): Diagnosismentioning

confidence: 99%

Section: Nipah Virus (Niv): Diagnosismentioning

confidence: 99%

Section: Nipah Virus (Niv): Diagnosismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cannabis sativa: 2023-Outbreak and Re-emergence of Nipah virus (NiV) in India: Role of Hemp oil

Ravindra B. Malabadi,

Kiran P. Kolkar,

Raju K. Chalannavar

et al. 2023

GSC Biol. Pharm. Sci.

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“…11,12 Bispecific antibodies (BsAb) could be obtained via chemical cross-linking, 13,14 molecular biology, 15,16 or hybrid−hybridoma technology. 17,18 Bispecific monoclonal antibodies (BsMAb) are monoclonals with two different specific antigen-binding sites, normally generated via hybrid−hybridomas. 19 BsMAb could specifically bind two different analytes using its two antigenbinding sites, and thus has a clear advantage in analyzing two contaminants.…”

Section: ■ Introductionmentioning

confidence: 99%

Bispecific Monoclonal Antibody-Based Multianalyte ELISA for Furaltadone Metabolite, Malachite Green, and Leucomalachite Green in Aquatic Products

Wang

Shen

et al. 2016

J. Agric. Food Chem.

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A new multi-analyte immunoassay was designed to screen furaltadone metabolite 5-morpholinomethyl-3-amino-2-oxazolidone (AMOZ), malachite green (MG), and leucomalachite green (LMG) in aquatic products using a bispecific monoclonal antibody (BsMAb). Gradient drug mutagenesis methods were separately used to prepare an anti-3-nitrobenzaldehyde-derivatized AMOZ (3-NPAMOZ) hybridoma cell line that was hypoxanthine-guanine-phosphoribosyltransferase (HGRPT) deficient and an anti-LMG hybridoma cell line that was thymidine kinase (TK) deficient. BsMAb recognizing 3-NPAMOZ and LMG was generated using hybrid-hybridomas of HGRPT and TK deficient cell lines. For AMOZ and LMG, respectively, the BsMAb-based indirect competitive ELSIA (ic-ELISA) values of 1.7 ng/mL and 45.3 ng/mL and detection limits of 0.2 ng/mL and 4.8 ng/mL. To establish the ic-ELISA, 3-NPAMOZ derivatized from AMOZ with 3-nitrobenzaldehyde and LMG reduced from MG by potassium borohydride was recognized by BsMAb. Recoveries of AMOZ, MG, and LMG in aquatic products were satisfactory and correlated with HPLC analysis. Thus, the multi-analyte ic-ELISA is suitable for rapid quantification of AMOZ, MG and LMG in aquatic products.

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Evaluation of direct versus multi-layer passivation and capture chemistries for nanoparticle-based biosensor applications

Sanjaya

Ranzoni

Watterson

et al. 2015

Biosensors and Bioelectronics

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Heterosandwich immunoswab assay for dengue virus Ns1 antigen detection

Cited by 5 publications

References 21 publications

Cannabis sativa: 2023-Outbreak and Re-emergence of Nipah virus (NiV) in India: Role of Hemp oil

Cannabis sativa: 2023-Outbreak and Re-emergence of Nipah virus (NiV) in India: Role of Hemp oil

Bispecific Monoclonal Antibody-Based Multianalyte ELISA for Furaltadone Metabolite, Malachite Green, and Leucomalachite Green in Aquatic Products

Evaluation of direct versus multi-layer passivation and capture chemistries for nanoparticle-based biosensor applications

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