Heterotrimeric G Protein Signaling in Filamentous Fungi

Li, Liande; Wright, Sara J.; Kryštofová, Svetlana; Park, Gyungsoon; Borkovich, Katherine A.

doi:10.1146/annurev.micro.61.080706.093432

Cited by 308 publications

(363 citation statements)

References 128 publications

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“…cAMP produced by the adenylyl cyclase can activate the PKA subunits CPK1 and CPK2, which in turn activate regulators through phosphorylation (Li et al, 2007). The expression of essential DON biosynthesis and transport genes TRI5, TRI6 and TRI12 was reduced in a ∆cpk1 KO, indicating a regulation of DON production through cAMP-PKA signalling (Hu et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…AreA in turn might be regulated by cAMP-PKA signalling (Hou et al, 2015). Generally, protein kinase A (PKA) is activated by cyclic 3',5'-adenosine monophosphate (cAMP) produced by adenylyl cyclases, which are regulated by G-proteins (Li et al, 2007). In F. graminearum G-protein α and β subunits (GPA1 & GPB1) and regulators of G protein signalling (FlbA & RgsA) are negative regulators of DON production, whereas the adenylyl cyclase FAC1 is an essential positive regulator of DON production (Park et al, 2012;Yu et al, 2008;Bormann et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production

Blum

Benfield

Stiller

et al. 2016

Fungal Genetics and Biology

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., High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production, Fungal Genetics and Biology (2016), doi: http://dx.doi. org/10.1016/j.fgb. 2016.02.005 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ABSTRACTFusarium head blight and crown rot, caused by the fungal plant pathogen Fusarium graminearum, impose a major threat to global wheat production. During the infection, plants are contaminated with mycotoxins such as deoxynivalenol (DON), which can be toxic for humans and animals. In addition, DON is a major virulence factor during wheat infection. However, it is not fully understood how DON production is regulated in F. graminearum. In order to identify regulators of DON production, a high-throughput mutant screen using Fluorescence Activated Cell Sorting (FACS) of a mutagenized TRI5-GFP reporter strain was established and a mutant over-producing DON under repressive conditions identified. A gain-of-function mutation in the F. graminearum adenylyl cyclase (FAC1), which is a known positive regulator of DON production, was identified as the cause of this phenotype through genome sequencing and segregation analysis. Our results show that the high-throughput mutant screening procedure developed here can be applied for identification of fungal proteins involved in diverse processes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production

Blum

Benfield

Stiller

et al. 2016

Fungal Genetics and Biology

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“…Fungal Ga proteins are divided into three groups according to their structure. S. cerevisiae Gpa1 and its orthologues in filamentous fungi belong to class I whereas Gpa2 belongs to class III (Li et al, 2007). In contrast, the two Ga proteins in S. pombe, Gpa1 and Gpa2, belong to classes II and III, respectively.…”

Section: The Fus3 and Kss1 Mapk Pathwaysmentioning

confidence: 99%

Comparative genomics of MAP kinase and calcium–calcineurin signalling components in plant and human pathogenic fungi

Rispail

Soanes

Ant

et al. 2009

Fungal Genetics and Biology

285

281

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a b s t r a c tMitogen-activated protein kinase (MAPK) cascades and the calcium-calcineurin pathway control fundamental aspects of fungal growth, development and reproduction. Core elements of these signalling pathways are required for virulence in a wide array of fungal pathogens of plants and mammals. In this review, we have used the available genome databases to explore the structural conservation of three MAPK cascades and the calcium-calcineurin pathway in ten different fungal species, including model organisms, plant pathogens and human pathogens. While most known pathway components from the model yeast Saccharomyces cerevisiae appear to be widely conserved among taxonomically and biologically diverse fungi, some of them were found to be restricted to the Saccharomycotina. The presence of multiple paralogues in certain species such as the zygomycete Rhizopus oryzae and the incorporation of new functional domains that are lacking in S. cerevisiae signalling proteins, most likely reflect functional diversification or adaptation as filamentous fungi have evolved to occupy distinct ecological niches.

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“…The cAMP pathway plays a central role in regulating cellular proliferation and metabolism in budding yeast (Talbot 2003). Upstream signal transducers that exert a regulatory influence on cAMP synthesis during the infection-related morphogenesis comprise a heterotrimeric G-proteins complex of Gα subunit (Mag A, Mag B and Mag C) (Li et al 2007), Gβ subunit (Mgb1 and Mgb2) and Gϒ subunit (Magg1) (Nishimura et al 2003). Regulator of G-protein signalling (RGS) negatively regulates all Gα Subunits in M. oryzae (Liu et al 2007).…”

Section: Signal Transducing Cascades Associated With Appressorium Formentioning

confidence: 99%

Regulatory network of genes associated with stimuli sensing, signal transduction and physiological transformation of appressorium inMagnaporthe oryzae

et al. 2018

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Rice blast caused by Magnaporthe oryzae is the most destructive disease affecting the rice production (Oryza sativa), with an average global loss of 10–30% per annum. Recent reports have indicated that the fungus also inflicts blast disease on wheat (Triticum aestivum) posing a serious threat to the wheat production. Due to its easily detected infectious process and manoeuvrable genetic manipulation, M. oryzae is considered a model organism for exploring the molecular mechanism underlying fungal pathogenicity during the pathogen–host interaction. M. oryzae utilises an infectious structure called appressorium to breach the host surface by generating high turgor pressure. The appressorium development is induced by physical and chemical cues which are coordinated by the highly conserved cAMP/PKA, MAPK and calcium signalling cascades. Genes involved in the appressorium development have been identified and well studied in M. oryzae, a summary of the working gene network linking stimuli sensing and physiological transformation of appressorium is needed. This review provides a comprehensive discussion regarding the regulatory networks underlying appressorium development with particular emphasis on sensing of appressorium inducing stimuli, signal transduction, transcriptional regulation and the corresponding developmental and physiological responses. We also discussed the crosstalk and interaction of various pathways during the appressorium development.

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Heterotrimeric G Protein Signaling in Filamentous Fungi

Cited by 308 publications

References 128 publications

High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production

High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production

Comparative genomics of MAP kinase and calcium–calcineurin signalling components in plant and human pathogenic fungi

Regulatory network of genes associated with stimuli sensing, signal transduction and physiological transformation of appressorium inMagnaporthe oryzae

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