Heterotrimeric G protein signalling in plant biotic and abiotic stress response

Zhang, Huili; Xie, Peng; Xu, Xiuhong; Xie, Qi; Yu, Feifei

doi:10.1111/plb.13241

Cited by 25 publications

(12 citation statements)

References 118 publications

(257 reference statements)

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“…Sugar signaling and phytohormones regulate the distribution of carbon assimilates in crops, especially under abiotic stress (Yu et al, 2015; Zhang et al, 2018b; Chen et al, 2022b). The heterotrimeric G protein has been found to be involved in sugar and phytohormone signaling in plants (Peng et al, 2018; Zhang et al, 2021). This might suggest that RGA1 can improve the source–sink relationship by regulating sugar and phytohormone signaling under low-light conditions.…”

Section: Discussionmentioning

confidence: 99%

RGA1 alleviates low-light-repressed pollen tube growth by improving the metabolism and allocation of sugars and energy

Li¹,

Feng²,

Li³

et al. 2022

Preprint

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Low-light stress compromises photosynthetic and energy efficiency and leads to spikelet sterility; however, the effect of low-light stress on pollen tube elongation in the pistil remains poorly understood. The gene RGA1, which encodes a Gα subunit of the heterotrimeric G protein, enhanced low-light tolerance in rice plants at anthesis by preventing the cessation of pollen tube elongation in the pistil. The levels of reactive oxygen species were higher and the content of ATP and ATPase was lower in RGA1 mutant (d1) plants compared with wild-type and RGA1-overexpressing (OE-1) plants under low-light conditions. Energy deficits, rather than interference with signaling transduction pathways, were the main contributors to the inhibition of pollen tube elongation in the pistil by low-light stress. In this process, marked increases in the activities of acid invertase (INV), sucrose synthase (SUS), and mitochondrial respiratory electron transport chain complexes, as well as the relative expression levels of SUTs, SWEETs, SUSs, INVs, CINs, SnRK1A, and SnRk1B, were observed in OE-1 plants. INV and ATPase activators (sucrose and Na2SO3, respectively) increased spikelet fertility by improving the energy status in the pistil under low-light conditions, and the ATPase inhibitor Na2VO4 induced spikelet sterility and decreased ATPase activity. Therefore, RGA1 could alleviate the low-light stress-induced impairment of pollen tube elongation to increase spikelet fertility by promoting sucrose unloading in the pistil and improving the metabolism and allocation of energy.

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Section: Discussionmentioning

confidence: 99%

RGA1 alleviates low-light-repressed pollen tube growth by improving the metabolism and allocation of sugars and energy

Li¹,

Feng²,

Li³

et al. 2022

Preprint

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show abstract

“…For example, drought can aggravate the damage caused by pests and increase the susceptibility of plants to infections, whereas excessive humidity leads to a more serious invasion by pathogenic bacteria [6][7][8][9][10][11][12]. Therefore, plants need to recognize or respond to biotic and abiotic stresses to deal with these environmental problems [13][14][15][16][17][18][19][20][21][22][23][24]. Recently, the functions of CLAVATA3/embryo-surrounding region-related (CLE) peptides and their receptors have been identified and characterized in regulating the pathogen invasion and drought stress of plants, suggesting that plants may utilize a single signaling pathway or strategy to respond to compound stress [25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

FLS2–RBOHD–PIF4 Module Regulates Plant Response to Drought and Salt Stress

Liu

Guo

et al. 2022

IJMS

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As sessile organisms, plants are constantly challenged by several environmental stresses. Different kinds of stress often occur simultaneously, leading to the accumulation of reactive oxygen species (ROS) produced by respiratory burst oxidase homolog (RBOHD) and calcium fluctuation in cells. Extensive studies have revealed that flagellin sensitive 2 (FLS2) can sense the infection by pathogenic microorganisms and activate cellular immune response by regulating intracellular ROS and calcium signals, which can also be activated during plant response to abiotic stress. However, little is known about the roles of FLS2 and RBOHD in regulating abiotic stress. In this study, we found that although the fls2 mutant showed tolerance, the double mutant rbohd rbohf displayed hypersensitivity to abiotic stress, similar to its performance in response to immune stress. An analysis of the transcriptome of the fls2 mutant and rbohd rbohf double mutant revealed that phytochrome interacting factor 4 (PIF4) acted downstream of FLS2 and RBOHD to respond to the abiotic stress. Further analysis showed that both FLS2 and RBOHD regulated the response of plants to drought and salt stress by regulating the expression of PIF4. These findings revealed an FLS2–RBOHD–PIF4 module in regulating plant response to biotic and abiotic stresses.

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“…In addition, they contain plant-specific Gproteins, such as EXTRA LARGE G-PROTEINS (XLG1, 2 and 3 in Arabidopsis), a class of alternative Gα subunits with an N-terminal domain of unknown function. Similar to metazoans, signal transduction by plant heterotrimeric G-proteins is required for many processes, including growth, development, hormone and sugar sensing as well as responses to biotic and abiotic stresses (reviewed by Pandey, 2019;Zhong et al, 2019;Ofoe, 2021;Zhang et al, 2021). However, bona fide GPCRs have not been identified in plants and relatively little is known about the regulatory mechanisms.…”

Section: Introductionmentioning

confidence: 99%

EXTRA LARGE G-PROTEIN 2 (XLG2) mediates cell death and hyperimmunity via a novel, apoplastic ROS-independent pathway inArabidopsis thaliana

Petutschnig

Anders

Stolze

et al. 2021

Preprint

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Heterotrimeric G-Proteins are signal transduction complexes comprised of three subunits, Gα, Gβand Gγ, and are involved in many aspects of plant life. The non-canonical Gα subunit XLG2 mediates PAMP-induced ROS generation and immunity downstream of PRRs. A mutant of the chitin receptor component CERK1, cerk1-4, maintains normal chitin signalling capacity, but shows excessive cell death upon infection with powdery mildews. We identified XLG2 mutants as suppressors of the cerk1-4 phenotype. We generated stably transformed Arabidopsis lines expressing Venus-XLG2 and numerous mutated variants. These were analysed by confocal microscopy, Western blotting and pathogen infection. We also crossed cerk1-4 with several mutants involved in immunity and analysed their phenotype. Phosphorylation of XLG2 was investigated by quantitative proteomics. Mutations in XLG2 complex partners AGB1 and AGG1 have a partial cerk1-4 suppressor effect. The cerk1-4 phenotype is independent of NADPH oxidase-generated ROS, BAK1 and SOBIR1, but requires PUB2. XLG2 mediates cerk1-4 cell death at the cell periphery. Integrity of the XLG2 N-terminal domain, but not its phosphorylation, is essential for correct XLG2 localisation and cerk1-4 signalling. Our results suggest that XLG2 transduces signals from an unknown cell surface receptor that activates an apoplastic ROS-independent cell death pathway in Arabidopsis.

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Heterotrimeric G protein signalling in plant biotic and abiotic stress response

Cited by 25 publications

References 118 publications

RGA1 alleviates low-light-repressed pollen tube growth by improving the metabolism and allocation of sugars and energy

RGA1 alleviates low-light-repressed pollen tube growth by improving the metabolism and allocation of sugars and energy

FLS2–RBOHD–PIF4 Module Regulates Plant Response to Drought and Salt Stress

EXTRA LARGE G-PROTEIN 2 (XLG2) mediates cell death and hyperimmunity via a novel, apoplastic ROS-independent pathway inArabidopsis thaliana

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