Heterotrophic ammonium assimilation: An important driving force for aerobic denitrification of Rhodococcus erythropolis strain Y10

Ma, S.; Huang, Shi‐Wei; Tian, Yun; Lu, Xiangyang

doi:10.1016/j.chemosphere.2021.132910

Cited by 35 publications

(5 citation statements)

References 46 publications

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“…Therefore, the optimal C/N ratio for strain S2, considering energy savings, was 16. These findings align with the high NH4 + -N removal capacity exhibited by Rhodococcus erythropolis Y10 [27] and Acinetobacter indicus ZJB20129 [3] with a C/N ratio of 15.…”

Section: Carbon-nitrogen Ratiosupporting

confidence: 80%

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A Newly Isolated Rhodococcus sp. S2 from Landfill Leachate Capable of Heterotrophic Nitrification and Aerobic Denitrification

Chen,

Li,

Zhang

et al. 2024

Water

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Nitrogen removal through heterotrophic nitrification–aerobic denitrification (HN–AD) has been acknowledged as one of the most efficient and cost-effective nitrogen removal processes. This study involved the isolation of a novel HN–AD bacterium (Rhodococcus sp. S2) from landfill leachate. Rhodococcus sp. S2 exhibited high nitrogen removal performance under aerobic conditions without the accumulation of nitrite as an intermediate. The maximum removal efficiencies for NH4+-N, NO2−-N, and NO3−-N were found to be 99.97 ± 0.3%, 99.79 ± 0.14%, and 83.53 ± 0.31%, respectively. Additionally, Rhodococcus sp. S2 demonstrated simultaneous nitrifying and denitrifying capabilities and showed a preference for utilizing NH4+-N in mixed nitrogen sources. The optimal nitrogen removal conditions for Rhodococcus sp. S2 were as follows: sodium acetate as a carbon source, a C/N ratio of 16, a shaking speed of 200 rpm, a pH of 9, and a temperature of 35 °C. Genome sequencing results revealed the presence of nitrate reductases (NarG), nitrate oxidoreductase (NxrA), and nitrite reductase (NirBD) in Rhodococcus sp. S2, providing further evidence of its HN–AD capability. In treating raw wastewater under the aforementioned experimental conditions, S2 achieved a maximum TN removal efficiency of 57.16 ± 0.52% with the addition of sodium acetate as a carbon source. These results suggest that Rhodococcus sp. S2 might be a promising candidate for wastewater nitrogen removal.

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Section: Carbon-nitrogen Ratiosupporting

confidence: 80%

“…However, only a few studies have confirmed the HN-AD capability of Rhodococcus sp. Previously, Rhodococcus erythropolis strain Y10 isolated from surface flow constructed wetlands (SFCWs) was proven to have heterotrophic ammonium assimilation and aerobic denitrification capacity [27]. Rhodococcus sp.…”

Section: Isolation and Identification Of Strain S2mentioning

confidence: 99%

A Newly Isolated Rhodococcus sp. S2 from Landfill Leachate Capable of Heterotrophic Nitrification and Aerobic Denitrification

Chen,

Li,

Zhang

et al. 2024

Water

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“…Rhodococcus can survive in hostile environments, such as those contaminated with polychlorinated biphenyl [30], nitrophenols, trichloroethene [31], and polycyclic aromatic hydrocarbons [32]. Studies have shown that Rhodococcus can degrade and remove aromatic [33], nitrile [34], sulfuric [35], and nitrogenous compounds [36] in the environment. Indeed, Rhodococcus are commonly used to remediate environmental contamination and pollution [37].…”

Section: Discussionmentioning

confidence: 99%

Effects of Cadmium Stress on Bacterial and Fungal Communities in the Whitefly Bemisia tabaci

Guo,

Li,

2023

IJMS

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Heavy metal contamination is among the most prominent environmental problems in China, posing serious threats to both ecosystem and human health. Among the diverse heavy metal contaminants, cadmium is the most serious. The whitefly Bemisia tabaci is a cosmopolitan pest capable of causing severe damage to a broad range of agricultural crops, especially vegetables. At present, little is known about the effects of cadmium stress on B. tabaci, including on its bacterial and fungal communities. In the current study, we investigated the effects of cadmium on bacterial and fungal communities in whiteflies. Meta-barcode sequencing of the 16S rRNA gene revealed that the whitefly bacterial community contained 264 operational taxonomic units (OTUs) belonging to 201 known genera and 245 known species. The top five most frequent bacterial genera were Rickettsia, Rhodococcus, Candidatus Portiera, Candidatus Hamiltonella, and Achromobacter. Meta-barcode sequencing of the fungal ITS locus revealed that the whitefly fungal community contained 357 OTUs belonging to 187 known genera and 248 known species. The top five most frequent fungal genera were Wallemia, unclassified_f_Dipodascaceae, Apiotrichum, Penicillium, and unclassified_o_Saccharomycetales. Cadmium exposure reduced the fungal OTU richness but increased the bacterial Shannon and Simpson diversity indices in whiteflies. In addition, upon exposure to cadmium, the microbial community composition in whiteflies changed significantly, with increased prevalence of the bacterial genera Rhodococcus and Exiguobacterium and fungal genus Wallemia. Our results indicate that the whitefly microbiota likely contributed to their adaptation and resistance to cadmium and suggested that whiteflies may contain microbes that could help remediate cadmium contamination in natural environments and agricultural fields.

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“…Assimilation synthesizes proteins and nucleic acids required for microbial growth, which are important for both cell growth and nitrogen conversion. It has been reported that R. erythropolis Y10 can use ammonium, nitrate, and nitrite via the assimilation pathway; 98.23% of the nitrogen is assimilated to biomass nitrogen with ammonium as the sole nitrogen source, much higher than the 52.73 and 56.22% for nitrate and nitrite, respectively (Ma et al, 2021). Vibrio sp.…”

Section: Assimilation and Dissimilation Of Nitrogen In Hnadsmentioning

confidence: 99%

“…YS2 (Lang et al, 2020), and mixed Bacillus strains (Kim et al, 2005). In addition, some HNADs such as Rhodococcus erythropolis Y10 (Ma et al, 2021) remove ammonia nitrogen by assimilation, converting the nitrogen source into biological nitrogen for their own growth.…”

Section: Distribution and Evolutionary Relationships Of Novel Hnad St...mentioning

confidence: 99%

Advances in Research Into and Applications of Heterotrophic Nitrifying and Aerobic Denitrifying Microorganisms

Song²,

Wang³

et al. 2022

Front. Environ. Sci.

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With the increasing use of animal and plant proteins, pollution due to nitrogen sources is attracting increasing attention. In particular, the amount of nitrogen-containing sewage discharged into the environment has increased significantly, causing eutrophication of water bodies and environmental degradation of water quality. Traditionally, nitrifying bacteria perform ammonia nitrification under aerobic conditions, while denitrifying bacteria perform nitrate/nitrite denitrification under anaerobic conditions. However, heterotrophic nitrifying and aerobic denitrifying microorganisms (HNADs) perform ammonia nitrification and nitrate/nitrite denitrification under the same aerobic conditions using an organic carbon source, which is a much simpler and more efficient process. In this review, the distribution and evolutionary relationships of novel HNADs strains are presented, and the influencing factors, metabolic pathways, key enzymes, and practical applications of HNADs are reviewed.

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Heterotrophic ammonium assimilation: An important driving force for aerobic denitrification of Rhodococcus erythropolis strain Y10

Cited by 35 publications

References 46 publications

A Newly Isolated Rhodococcus sp. S2 from Landfill Leachate Capable of Heterotrophic Nitrification and Aerobic Denitrification

A Newly Isolated Rhodococcus sp. S2 from Landfill Leachate Capable of Heterotrophic Nitrification and Aerobic Denitrification

Effects of Cadmium Stress on Bacterial and Fungal Communities in the Whitefly Bemisia tabaci

Advances in Research Into and Applications of Heterotrophic Nitrifying and Aerobic Denitrifying Microorganisms

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