Heterotrophic Cultivation of the Cyanobacterium Pseudanabaena sp. on Forest Biomass Hydrolysates toward Sustainable Biodiesel Production

Abstract

Environmental pollution, greenhouse gas emissions, depletion of fossil fuels, and a growing population have sparked a search for new and renewable energy sources such as biodiesel. The use of waste or residues as substrates for microbial growth can favor the implementation of a biorefinery concept with reduced environmental footprint. Cyanobacteria constitute microorganisms with enhanced ability to use industrial effluents, wastewaters, forest residues for growth, and concomitant production of added-value compounds. In this study, a recently isolated cyanobacterium strain of Pseudanabaena sp. was cultivated on hydrolysates from pretreated forest biomass (silver birch and Norway spruce), and the production of biodiesel-grade lipids was assessed. Optimizing carbon source concentration and the (C/N) carbon-to-nitrogen ratio resulted in 66.45% w/w lipid content when microalgae were grown on glucose, compared to 62.95% and 63.79% w/w when grown on spruce and birch hydrolysate, respectively. Importantly, the lipid profile was suitable for the production of high-quality biodiesel. The present study demonstrates how this new cyanobacterial strain could be used as a biofactory, converting residual resources into green biofuel.

Development of Meat Substitutes from Filamentous Fungi Cultivated on Residual Water of Tempeh Factories

“…As shown in Figure 2 , the addition of yeast extract resulted in the highest fungal biomass, which reached 5.27 g/L (dry basis), increasing the production by two and half times. By adding yeast extract containing 10% nitrogen [ 20 ], the C/N ratio was decreased, favoring biomass production [ 21 ]. In addition, yeast extract contains 2.5% phosphorus and a broad range of macro- and micronutrients [ 20 ], which could provide the nutritional requirement for the growth of filamentous fungi.…”