Heterotrophic Microalgal Cultivation

Lee, Dong Hoon; Chang, Jo Shu

doi:10.1007/978-981-10-7677-0_4

Cited by 11 publications

(4 citation statements)

References 204 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, the microalgae are able to initiate complementary metabolisms during natural day–night cycles; , for example, the storage compounds (e.g., glycogen and poly-P) produced during daytime can be utilized to support the microalgal growth during nighttime . Thus, microalgae may grow heterotrophically using an organic substrate under dark conditions with simultaneous ammonia and phosphorus removal. − In addition, the facultative bacteria may transform the complex organics to small molecular weight organic carbon sources for microalgal assimilation under dark conditions. These imply that a synergetic effect between microalgae and bacteria may exist even at night, while the performances and mechanisms of the MBGS process in wastewater treatment under dark conditions, including the mass and energy balance during varied natural day-night cycles, need further study.…”

Section: Performance Of Mbgs Under Light and Dark Conditionsmentioning

confidence: 99%

Assessment of Microalgal-Bacterial Granular Sludge Process for Environmentally Sustainable Municipal Wastewater Treatment

Liu

2021

ACS EST Water

View full text Add to dashboard Cite

The conventional municipal wastewater treatment processes are facing increasing pressures due to their huge energy consumption, significant emissions of greenhouse gases, and low resource recovery potential. As such, the microalgal–bacterial granular sludge (MBGS) process has recently been explored with the aim for concurrent high-efficiency water, energy, and resource recovery from municipal wastewater in an environmentally sustainable manner. This review attempts to offer a holistic view of the state of the art techniques of MBGS process for municipal wastewater treatment. It was shown that mutualism and symbiosis between microalgae and bacteria could determine the physical structure and microbial community of MBGS. Microbial assimilation instead of dissimilation was identified to be the main mechanisms for removing soluble organics, ammonia, and phosphate in municipal wastewater under both light and dark conditions. Different from the conventional biological nutrients removal processes, wastewater nutrients were effectively fixed into MBGS which could be regarded as a potential source for further energy and resource recovery. Compared to the conventional activated sludge process, the energy consumption and carbon emissions would be reduced, respectively, by 100% and 63% by adopting MBGS. It is apparent that MBGS could offer an alternative toward energy- and carbon-neutral municipal wastewater treatment.

show abstract

Section: Performance Of Mbgs Under Light and Dark Conditionsmentioning

confidence: 99%

Assessment of Microalgal-Bacterial Granular Sludge Process for Environmentally Sustainable Municipal Wastewater Treatment

Liu

2021

ACS EST Water

View full text Add to dashboard Cite

show abstract

“…During photoautotrophic cultivation, microalgae could assimilate only atmospheric CO 2 , while during mixotrophic cultivation, microalgae assimilate CO 2 and organic carbon simultaneously 19 . Thus, photoautotrophic cultivation of microalgae results in comparatively lower biomass productivity 20 …”

Section: Introductionmentioning

confidence: 99%

“…19 Thus, photoautotrophic cultivation of microalgae results in comparatively lower biomass productivity. 20 Various agro-industrial wastes are produced in massive proportions throughout the world and usually dumped in the ambient environment as point/nonpoint source pollutants. [21][22][23][24][25][26][27] Such wastes are generally available at low/no cost and can be valorized as organic carbon sources for the mixotrophic cultivation of microalgae.…”

Section: Introductionmentioning

confidence: 99%

Mixotrophic cultivation of Chlorella vulgaris in sugarcane molasses preceding nitrogen starvation: Biomass productivity, lipid content, and fatty acid analyses

Laraib

Manzoor

Javid

et al. 2021

Env Prog and Sustain Energy

View full text Add to dashboard Cite

In the present study, a precharacterized oleaginous microalgal species (Chlorella vulgaris) was exploited for its biomass productivity, lipid yield, and fatty acid analysis under mixotrophic and photoautotrophic conditions. For the purpose, the microalgal biomass was raised mixotrophically as well photoautotrophically. For mixotrophic cultivation, molasses was used as additional source of carbon and energy, while for photoautotrophic cultivation, atmospheric CO 2 was the sole source of carbon and energy. The microalgal biomass raised thus was then harvested following 5 days of nitrogen starvation. Mixotrophic cultivation depicted remarkably higher biomass productivity (137.43 ± 13.3 mg L −1 day −1 ) than that of photoautotrophic cultivation (91.57 ± 7.9 mg L −1 day −1 ). About 39% (w/w) of total lipid content was obtained from the dried biomass raised mixotrophically. Lipidomic analysis of the extracted oil depicted higher concentrations of polyunsaturated fatty acids (palmitic and oleic acids). The significant availability of polyunsaturated fatty acids (60 mg g −1 of dried algal biomass) ensured the production of high quality biofuel.

show abstract

“…While closed photobioreactors are an alternative to open ponds, these invariably have high start-up and maintenance costs, are easily fouled by algae growing on the clear surfaces, and often become contaminated with fungi or bacteria that are difficult to eliminate (Chisti 2007;Carvalho et al 2008;Xu et al 2009;Richardson et al 2012;Louw et al 2016;Kern et al 2017). An appropriate alternative is to grow algae heterotrophically in dark bioreactors supplemented with sugars, preferably from waste biomass hydrolysates (Perez-Garcia et al 2011;Nagarajan et al 2018). Heterotrophic algal growth obviates the problems imposed by light penetration and water loss, while producing biodiesel lipids of high quality.…”

Section: Introductionmentioning

confidence: 99%

Effects of nitrogen and phosphorus limitation on lipid accumulation by Chlorella kessleri str. UTEX 263 grown in darkness

2020

View full text Add to dashboard Cite

Growing algae in darkness for biodiesel production eliminates the challenges of evaporation and light penetration reported for open ponds and the costs and fouling that plague photobioreactors. The current study demonstrated that Chlorella kessleri str. UTEX 263 could grow heterotrophically in the dark on pure sugars or lignocellulosic hydrolysates of plant biomass. Hydrolysates of a prairie grass native to Kansas, Big bluestem (Andropogon gerardii), supported the growth of C. kessleri in the dark. Nitrogen limitation stimulated the accumulation of biodiesel lipids by 10-fold in heterotrophic cultures grown on pure sugars or Big bluestem hydrolysate. Limiting P in the growth medium also was shown to increase cellular lipid accumulation in C. kessleri. Iron limitation was not sufficient to increase cellular lipid content. Crude biomass extracts may have levels of N that can't be easily removed, which are high enough to relieve N limitations in growth media. This initial study suggests that P might be more easily removed from biomass extracts than N for increasing cellular lipid production by nutrient limitation and further that native prairie grasses are potentially suitable as sources of lignocellulosic sugars.

show abstract

Heterotrophic Microalgal Cultivation

Cited by 11 publications

References 204 publications

Assessment of Microalgal-Bacterial Granular Sludge Process for Environmentally Sustainable Municipal Wastewater Treatment

Assessment of Microalgal-Bacterial Granular Sludge Process for Environmentally Sustainable Municipal Wastewater Treatment

Mixotrophic cultivation of Chlorella vulgaris in sugarcane molasses preceding nitrogen starvation: Biomass productivity, lipid content, and fatty acid analyses

Effects of nitrogen and phosphorus limitation on lipid accumulation by Chlorella kessleri str. UTEX 263 grown in darkness

Contact Info

Product

Resources

About