Heterotypic cell-cell adhesion of human mast cells to fibroblasts

Trautmann, Axel; Feuerstein, Bernadette; Ernst, Norman; Bröcker, Eva B.; Klein, Corinna

doi:10.1007/s004030050180

Cited by 38 publications

(51 citation statements)

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“…The degree of adhesion demonstrated under basal conditions, ϳ45% of mast cells, was high compared with that reported for T cells and eosinophils (19,20), and similar to that described previously for HLMC adhesion to human bronchial epithelium (7) and human skin mast cell adhesion to human skin fibroblasts (21).…”

Section: Discussionsupporting

confidence: 53%

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Human Lung Mast Cells Adhere to Human Airway Smooth Muscle, in Part, via Tumor Suppressor in Lung Cancer-1

Yang

Kaur

Okayama

et al. 2006

The Journal of Immunology

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Mast cells infiltrate the airway smooth muscle (ASM) of patients with asthma, an event which is likely to be a key factor in the development of this disease. Adhesion is a fundamental mechanism facilitating cellular cross-talk. We have examined whether human lung mast cells (HLMC) and ASM adhere, and have also examined the mechanism involved. Primary cultures of HLMC and confluent human ASM were cocultured for 30 min, then nonadherent HLMC were removed by centrifugation. HLMC adhered avidly to ASM monolayers (mean ± SEM adhesion 43.2 ± 1.2%, n = 41). Adhesion was increased to 58.8 ± 2.7% by 1 mM Mn2+ (p = 0.015), and was reduced by EDTA and EGTA to 20.5 ± 1.5% and 21.0 ± 1.3%, respectively (p < 0.0001). Adhesion-blocking Abs for ICAM-1, VCAM-1, CD18, and the α4 and β1 integrins had no effect on HLMC adhesion. HLMC expressed tumor suppressor in lung cancer-1 (TSLC-1) and blocking this reduced adhesion from 38.5 ± 4.8% to 28.3 ± 3.7% (p = 0.004, n = 7). ASM did not express TSLC-1, indicating that TSLC-1 acts as a heterophilic adhesion molecule. In summary, HLMC adhere avidly to ASM in part via TSLC-1 and in part via an as-yet-undefined Ca2+-dependent pathway. This supports the hypothesis that adhesion is important in the recruitment and retention of HLMC by the ASM in asthma, and for the functional interaction of these cells.

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Section: Discussionsupporting

confidence: 53%

“…However, these molecules are also expressed by human bronchial epithelium and fibroblasts but do not mediate human mast cell adhesion to these cells (7,21). We previously have undertaken an extensive investigation into the adhesion of HLMC to airway epithelial cells (7).…”

Section: Discussionmentioning

confidence: 99%

Human Lung Mast Cells Adhere to Human Airway Smooth Muscle, in Part, via Tumor Suppressor in Lung Cancer-1

Yang

Kaur

Okayama

et al. 2006

The Journal of Immunology

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“…40 Heterotypic mast cell contact with other cells has been reported to influence mast cell responses, leaving this as a possibility. [41][42][43][44] Our final hypothesis suggests the potential interaction of ␣2␤1 integrin with certain microbial pattern recognition molecules of the innate immune system functioning with immune complexes. Collectins, ficolins, and the C1q complement protein all contain collagen-like sequences and are known to coat the surface of microbes.…”

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confidence: 99%

Mast cell–mediated inflammatory responses require the α2β1 integrin

Edelson

Pappan

et al. 2004

Blood

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Although the ␣2␤1 integrin is widely expressed and has been extensively studied, it has not been previously implicated in mast cell biology. We observed that ␣2 integrin subunit-deficient mice exhibited markedly diminished neutrophil and interleukin-6 responses during Listeria monocytogenes-and zymosan-induced peritonitis. Since exudative neutrophils of wildtype mice expressed little ␣2␤1 integrin, it seemed unlikely that this integrin mediated neutrophil migration directly. Here, we demonstrate constitutive ␣2␤1 integrin expression on peritoneal mast cells. Although ␣2-null mice contain normal numbers of peritoneal mast cells, these ␣2-null cells do not support in vivo mast cell-dependent inflammatory responses.We conclude that ␣2␤1 integrin provides a costimulatory function required for mast cell activation and cytokine production in response to infection. IntroductionThe ␣2␤1 integrin, a receptor for collagen, laminin, and other nonmatrix ligands, is expressed on a number of distinct cell types including epithelial cells, endothelial cells, fibroblasts, platelets, and leukocytes. 1 On cells of the immune system, the ␣2␤1 integrin is expressed on a subset of activated T lymphocytes (hence the designation very late activation antigen-2), on natural killer (NK) cells (DX5, originally defined as an NK cell-specific marker, was recently shown to recognize the ␣2␤1 integrin), and on neutrophils (polymorphonuclear leukocytes [PMNs]). 2,3 Although the role of the ␣2␤1 integrin in immune regulation is poorly understood, accumulating evidence suggests that this integrin may play an important role in leukocyte adhesion and extravasation from the vasculature into peripheral tissues. [4][5][6] Recently, we generated an ␣2 integrin subunit-deficient mouse model to investigate the role of the ␣2␤1 integrin in normal development and the pathogenesis of disease. 7 Here, we report that the ␣2 integrin-deficient mouse demonstrates a profound and surprising defect in the innate immune response during acute peritonitis. ␣2 integrin-deficient mice exhibit markedly diminished inflammatory responses to both Listeria monocytogenes, a gram-positive bacterium, and zymosan, a fungal polysaccharide. Although the PMNs of the ␣2 integrin-deficient mouse lack expression of this integrin, the inflammatory defect in these mice is not due to an inability of the PMNs to extravasate into the peritoneum. Instead, the ␣2␤1 integrin is expressed at high levels on all peritoneal mast cells (PMCs), a cell type required for the induction of the inflammatory response to infection. 8,9 We demonstrate that ␣2␤1 integrin expression on the PMC is required for mast cell activation and cytokine release in response to a number of agents in vivo and that ␣2␤1 integrin expression on the PMC is necessary for induction of normal inflammatory responses. Materials and methods Mice␣2 integrin-deficient mice on a C57BL/6 ϫ 129/Sv background were used at 6 to 20 weeks of age, with wild-type littermate controls. 7 Mice were maintained and bred under specific pathogen-...

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“…Skin mast cells express low levels of integrins α4β1 and α5β1 (less than 10%), while almost all mast cells from the uterus and intestines are positive for these integrins. On the other hand, human mast cell line HMC-1 expresses integrins β1 and α2 through α6, although their expression levels are variable [11,21].…”

Section: Discussionmentioning

confidence: 99%

“…Although the precise cause of this is unclear, it might have been due to failure of integrins on the MCT cells to function as Coll receptors, unsuitability of the substrate origin for the action, or unsuitability of the activating stimuli. Few mast cells have been found to adhere to Coll despite many experiments [11,21], and therefore low adhesion to Coll might be a characteristic of mast cells.…”

Section: Discussionmentioning

confidence: 99%

Role of .BETA.1 Integrins in Adhesion of Canine Mastocytoma Cells to Extracellular Matrix Proteins

Takahashi

Ohashi

Nakagawa

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. The interactions of tumor cells with the extracellular matrix (ECM) are a crucial step in invasion and metastasis. Integrins are adhesive molecules forming heterodimers with α and β subunits that play a definitive role in these interactions. In this study, mastocytoma (mast cell tumor: MCT) cell-ECM interaction was investigated using 3 canine MCT cell lines: CM-MC (originating from cutaneous MCT), VI-MC (originating from intestinal MCT), and CoMS (originating from oral MCT). Flow cytometric analysis showed that all cells highly expressed the integrin β1 and α1 through α5 subunits and that they moderately expressed the α6 subunit. In adhesion studies, CoMS weakly but spontaneously adhered to fibronectin (FN), which was enhanced by phorbol ester (TPA), while CM-MC and VI-MC required cell activation by TPA to adhere to FN. Anti-β1 and α5 integrin antibodies strongly inhibited cell adhesion to FN in CM-MC and CoMS and moderately inhibited cell adhesion in VI-MC. Only VI-MC adhered to laminin (LN) under activation by TPA. Anti-β1 integrin antibodies strongly inhibited cell adhesion to LN, but all anti-α integrin antibodies failed to inhibit cell adhesion to LN. No cells adhered to collagen types I and IV. Canine MCT cells from different origins expressed similar integrin patterns; however, there were some differences in adhesive behavior in response to various ECM proteins and activating stimuli.KEY WORDS: canine, extracellular matrix, integrin β1 subfamily, mastocytoma cell.

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Heterotypic cell-cell adhesion of human mast cells to fibroblasts

Cited by 38 publications

References 0 publications

Human Lung Mast Cells Adhere to Human Airway Smooth Muscle, in Part, via Tumor Suppressor in Lung Cancer-1

Human Lung Mast Cells Adhere to Human Airway Smooth Muscle, in Part, via Tumor Suppressor in Lung Cancer-1

Mast cell–mediated inflammatory responses require the α2β1 integrin

Role of .BETA.1 Integrins in Adhesion of Canine Mastocytoma Cells to Extracellular Matrix Proteins

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