Heterotypic Lymphoproliferative Response in Pigs Vaccinated With Foot-and-mouth Disease Virus. Involvement of isolated Capsid Proteins

Saiz, Juan-Carlos; Rodrı́guez, Ana Valdeolmillos; González, Miguel; Alonso, Fernando; Sánchez‐Madrid, Francisco

doi:10.1099/0022-1317-73-10-2601

Cited by 42 publications

(28 citation statements)

References 35 publications

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“…However, little is known on the immunological response and immune dynamics, vaccinecytokines response and how they contribute to a protective response against lumpy skin disease is available against this disease. To prevention and control of lumpy skin disease, several Capri poxvirus-vaccine strains are currently used, and despite of regular LSD vaccination of cattle, the cases of vaccine failure and re-occurrence of the disease have been reported (29). In the present study, the commercially available goat pox vaccine elicited a stronger immune response in the cattle than the sheep pox vaccine.…”

Section: Discussionmentioning

confidence: 55%

Evaluation of Humoral and Cell-mediated Immunity of Two Capripoxvirus Vaccine Strains against Lumpy Skin Disease Virus

et al. 2016

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Background and Aims: Prevention of Lumpy skin disease (LSD) in cattle is heavily dependent on vaccination. Since the genetic structure of LSD virus has the close relatedness with other Capri-pox virus (CaPV). Therefore, the use of vaccine strains of CaPV derived from sheep and goat would be useful to protect cattle against LSD. Materials and Methods:The susceptible calves of dairy farms were vaccinated with two available LSD vaccines. These vaccines were live attenuated sheep pox and goat pox vaccine strains. To evaluate vaccine-induced immune responses, whole blood and serum samples were collected up to 5 weeks post vaccination from both vaccinated and control groups. Results: The findings showed that, lymphocyte proliferation index in response to recall antigen in goat pox vaccine was higher than sheep pox vaccine in all time-point of experiments, and this difference was significant in weeks 1 and 5 post vaccination (p<0.05). Although the levels of antibody production in both vaccinated groups was almost the similar, and there was no statistically significant difference, but in goat pox vaccine slightly higher than sheep pox vaccine. Also, the interferon gamma and IL-4 production in goat pox vaccine were higher than sheep pox vaccine in all-time point and statistically significant at week 3 post vaccination (p< 0.05). Conclusions: From this study we found that live attenuated goat pox vaccine induced high level of lymphocyte proliferation and interferon gamma and IL-4, so it considered good vaccine to control of Lumpy skin disease.

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Section: Discussionmentioning

confidence: 55%

Evaluation of Humoral and Cell-mediated Immunity of Two Capripoxvirus Vaccine Strains against Lumpy Skin Disease Virus

et al. 2016

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“…Inactivated vaccines elicit a consistent humoral response, albeit generally weaker than that induced in infected animals [18]. This response correlates with a solid lymphoproliferative response [56,102,205], and with considerable, although short-lived, protection [18,72]. However, the spectrum of antiviral immune mechanisms induced by inactivated vaccines is limited [18,72].…”

Section: Conventional Vaccinesmentioning

confidence: 99%

“…In cattle and pigs, B cell activation and antibody production are associated with a lymphoproliferative response mainly mediated by T cells (mostly CD4 + ). These T helper cells recognise a number of viral epitopes, located both in capsid proteins [31,56,58,102,189,205,238,240] and NSP [59]. Thus, it appears that the T cell responses mediated by CD4 + cells are required for protective immunity against FMDV, by participating in the production of antiviral antibodies [31,58,240] and by maintaining the appropriate microenvironment needed for a synergistic immune response.…”

Section: Immune Responsementioning

confidence: 99%

“…Among the different attempts to improve the immunogenicity of these peptides, its expression as part of the hepatitis B core structure was the most promising [54]. In 1986, DiMarchi et al [70] reported protection to viral challenge in cattle immunised with a peptide in which the amino acids of the G-H loop were co-linearly synthesised with those corresponding to the carboxy terminus of VP1 (residues [200][201][202][203][204][205][206][207][208][209][210][211][212][213]. The induction of significant levels of heterologus protection in the guinea-pig with this tandem peptide has also been reported [73].…”

Section: Peptide Vaccinesmentioning

confidence: 99%

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Foot-and-mouth disease virus: a long known virus, but a current threat

et al. 2001

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-Foot-and-mouth disease virus (FMDV) was the first animal virus identified. Since then, FMDV has become a model system in animal virology and a considerable amount of information on its structure, biology and vaccinology has been obtained. However, the disease that this virus produces (FMD) still constitutes one of the main animal health concerns. In this review, we have attempted to summarise the state of the knowledge in different basic and applied areas of FMDV research, with emphasis on those aspects relevant to the control of the disease. FMDV / structure / immunity / vaccine / variability / diagnosisRésumé -Le virus de la fièvre aphteuse : un virus connu de longue date, qui demeure une menace. Le virus de la fièvre aphteuse a été le premier virus animal identifié. Depuis lors, il est devenu un système modèle en virologie animale, et une quantité importante d'informations sur sa structure, sa biologie et sa vaccinologie a été obtenue. Cependant la maladie provoquée par ce virus constitue encore une inquiétude majeure en santé animale. Dans cette revue, nous avons tenté de résumer l'état des connaissances dans différents domaines de recherche, à la fois fondamentaux et appliqués, sur le virus de la fièvre aphteuse, en mettant l'accent sur les aspects relatifs au contrôle de la maladie. virus de la fièvre aphteuse / immunité / vaccin / variabilité / diagnostic

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“…Şap hastalığına karşı korunmada, antikorların rol aldığı ileri sürülmüşse de (32), son yıllarda yapılan immunolojik çalışmalar sonucunda hücre aracılı bağışık-lık cevaplarının, özellikle de T-hücre cevabının önemli olduğu ortaya konmuştur (6,15,20). Virus ile enfeksiyonu ya da aşılamayı takiben, CD4+ ve CD8+ hücreleri içeren spesifik T-hücre aracılı antiviral cevapların şekil-lendiği ve virusun organizmadan uzaklaştırılmasında hücre-aracılı bağışıklığın rol aldığı rapor edilmiştir (5,24).…”

Section: Introductionunclassified

Şap hastalıklı koyunlarda serum nitrik oksit düzeyi ve adenozin deaminaz aktivitesinin araştırılması

NİSBET¹

2006

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu çalışmanın amacı, koyunlarda şap hastalığının serum nitrik oksit (NO) düzeyi ve adenozin deaminaz (ADA) aktivitesi üzerine etkilerini ve bu parametreler ile kan lenfosit ve monosit sayıları arasındaki ilişkileri incelemektir. Çalışmada 2-3 yaşlı 40 adet şap hastalığı virusu ile enfekte ve 20 adet klinik olarak sağlıklı koyun kullanıldı. Koyunların vena jugularisinden antikoagulanlı ve antikoagulansız tüplere kan örnekleri toplandı. Antikoagulanlı tüpteki kan örneğinde kan sayım cihazı ile lenfosit ve monosit sayımı yapıldı. Antikoagulansız tüpteki kanlar santrifüj edilerek serumları çıkarıldı. Serumda NO düzeyi enzimatik Greiss yöntemi, ADA aktivitesi Bertholet reaksiyonu ile spektrofotometrik olarak ölçüldü. Hastalıklı ve sağlıklı koyunlarda, serum NO düzeyleri ile ADA aktiviteleri, sırası ile, 40.0±7.3µmol/L ve 17.2±4.0 µmol/L, 17.6±4.1 U/L ve 10.2±2.1 U/L olarak belirlendi. Şap hastalıklı koyunlarda serum nitrik oksit düzeyi ile adenozin deaminaz enzim aktivitesi sağlıklı gruba göre önemli derecede yüksek bulundu (p<0.001). Kandaki lenfosit ve monosit sayıları (x10 3 /µL) hastalıklı koyunlarda, sırasıyla, 29.3±8.9 ve 9.6±3.3, sağlıklı koyunlarda ise 4.0±0.4 ve 1.1±0.5 olarak ölçüldü. Hastalıklı koyunların kan lenfosit ve monosit sayıları ile sağlıklı koyunlarınki arasında p<0.001 düzeyinde önemli farklılık olduğu belirlendi.Bu sonuçların yanında, hastalıklı koyunlarda serum NO düzeyi, ADA aktivitesi, kan lenfosit ve monosit sayıları arasında önemli düzeyde pozitif ilişkiler olduğu belirlendi. Sonuç olarak, şap hastalıklı koyunlarda serum NO ve ADA düzeylerindeki artışın, uyarılmış hücre aracılı bağışıklık sistemi ile ilgili olduğu ve bu parametrelerin şap hastalığının tanısında yardımcı olarak kullanılabi-leceği kanaatine varıldı.Anahtar sözcükler: Adenozin deaminaz, koyun, nitrik oksit, serum, şap hastalığı. The investigation of the effect of foot and mouth disease on nitric oxide levels and adenosine deaminase activity in sheepSummary: The aim of this study is to investigate the effects of foot and mouth disease on serum nitric oxide (NO) levels and adenosine deaminase (ADA) activity and correlations between these parameters and blood lymphocyte and monocyte numbers in sheep. 2-3 years old 40 sheep with foot and mouth disease and 20 sheep with healthy were used in the study. Blood was collected from jugular vein into the with and without-anticoagulant tubes from sheep. Lymphocyte and monocyte counts were made in with anticoagulant tubes by cell-counter. Serum was removed from without-anticoagulant by centrifugation. Serum NO level was measured by enzymatic Greiss, ADA activity by Bertholet reaction by spectrophotometrically. Serum NO levels and ADA activities were determined as 40.0±7.3µmol/L and 17.2±4.0 µmol/L, 17.6±4.1 U/L and 10.2±2.1 U/L, respectively. Serum NO levels and ADA activities were found to be significantly higher in sheep with foot and mouth disease compared to healthy (p<0.001). Blood lymphocyte and monocyte numbers determined as 29.3±8.9 and 9.6±3.3, 4.0±0.4 and 1.1±0.5 in sheep w...

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Heterotypic Lymphoproliferative Response in Pigs Vaccinated With Foot-and-mouth Disease Virus. Involvement of isolated Capsid Proteins

Cited by 42 publications

References 35 publications

Evaluation of Humoral and Cell-mediated Immunity of Two Capripoxvirus Vaccine Strains against Lumpy Skin Disease Virus

Evaluation of Humoral and Cell-mediated Immunity of Two Capripoxvirus Vaccine Strains against Lumpy Skin Disease Virus

Foot-and-mouth disease virus: a long known virus, but a current threat

Şap hastalıklı koyunlarda serum nitrik oksit düzeyi ve adenozin deaminaz aktivitesinin araştırılması

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