Hexameric Supramolecular Scaffold Orients Carbohydrates To Sense Bacteria

Grünstein, Dan; Maglinao, Maha; Kikkeri, Raghavendra; Collot, Mayeul; Barylyuk, Konstantin; Lepenies, Bernd; Kamena, Faustin; Zenobi, Renato; Seeberger, Peter H.

doi:10.1021/ja2036767

Cited by 81 publications

(70 citation statements)

References 72 publications

(38 reference statements)

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“…As a result, multiple weak binding interactions can appear to have a much higher effective binding energy. While this avidity-based, multivalent strategy has been widely applied in the field of nanobiotechnology for drug delivery, bioimaging, and biosensing applications,[8-10] it has yet to be explored in peptide hetero-assembled hydrogels.…”

Section: Introductionmentioning

confidence: 99%

Avidity-controlled hydrogels for injectable co-delivery of induced pluripotent stem cell-derived endothelial cells and growth factors

Mulyasasmita

Cai

Dewi

et al. 2014

Journal of Controlled Release

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To translate recent advances in induced pluripotent stem cell biology to clinical regenerative medicine therapies, new strategies to control the co-delivery of cells and growth factors are needed. Building on our previous work designing Mixing-Induced Two-Component Hydrogels (MITCH) from engineered proteins, here we develop protein-polyethylene glycol (PEG) hybrid hydrogels, MITCH-PEG, which form physical gels upon mixing for cell and growth factor co-delivery. MITCH-PEG is a mixture of C7, which is a linear, engineered protein containing seven repeats of the CC43 WW peptide domain (C), and 8-arm star-shaped PEG conjugated with either one or two repeats of a proline-rich peptide to each arm (P1 or P2, respectively). Both 20 kDa and 40 kDa star-shaped PEG were investigated, and all four PEG variants were able to undergo a sol-gel phase transition when mixed with the linear C7 protein at constant physiological conditions due to noncovalent hetero-dimerization between the C and P domains. Due to the dynamic nature of the C-P physical crosslinks, all four gels were observed to be reversibly shear-thinning and self-healing. The P2 variants exhibited higher storage moduli than the P1 variants, demonstrating the ability to tune the hydrogel bulk properties through a biomimetic peptide-avidity strategy. The 20 kDa PEG variants exhibited slower release of encapsulated vascular endothelial growth factor (VEGF), due to a decrease in hydrogel mesh size relative to the 40 kDa variants. Human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs) adopted a well-spread morphology within three-dimensional MITCH-PEG cultures, and MITCH-PEG provided significant protection from cell damage during ejection through a fine-gauge syringe needle. In a mouse hindlimb ischemia model of peripheral arterial disease, MITCH-PEG co-delivery of hiPSC-ECs and VEGF was found to reduce inflammation and promote muscle tissue regeneration compared to a saline control.

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Section: Introductionmentioning

confidence: 99%

Avidity-controlled hydrogels for injectable co-delivery of induced pluripotent stem cell-derived endothelial cells and growth factors

Mulyasasmita

Cai

Dewi

et al. 2014

Journal of Controlled Release

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“…Other carbohydrate-decorated fluorescent systems, such as dendrimers [111], peptide-based glycoclusters [112,113], glycosylated pyrene-graphene assembly [114], supramolecular scaffolds [115], and chitin nanocrystals [116] were also developed towards lectin binding and bacterial detection. These designs allow for the glycocluster effect to maximize the interaction between glycans and lectins as well as bacterial surface glycanbinding proteins.…”

Section: Glycan Decorated Fluorescent Polymersmentioning

confidence: 99%

Fluorescently labelled glycans and their applications

Yan

Yalagala

Yan³

2015

Glycoconj J

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This review summarises the literature on the synthesis and applications of fluorescently labelled carbohydrates. Due to the sensitivity of fluorescent detection, this approach provides a useful tool to study processes involving glycans. A few general categories of labelling are presented, in situ labelling of carbohydrates with fluorophores, fluorescently labelled glycolipids, fluorogenic glycans, pre-formed fluorescent glycans for intracellular applications, glycan-decorated fluorescent polymers, fluorescent glyconanoparticles, and other functional fluorescent glycans.

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“…As with the magnetic property, the fluorescent property of metalloglycoclusters was also utilized for selective cell imaging. Gruenstein et al succeeded in imaging bacteria expressing Fim-H protein and the results of their research suggest that metalloglycoclusters can be employed for the detection of bacteria (31).…”

Section: D-1 Affinity Evaluation Using Metalloglycoclustersmentioning

confidence: 99%

Application of Functionalized Metalloglycoclusters

Okada

2011

TIGG

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Metalloglycoclusters, part of the glycocluster family, are an attractive group of functional molecules comprising a metal complex decorated with carbohydrate moieties. Metalloglycoclusters possessing both organic and inorganic components show unique fluorescent, electrochemical and magnetic properties absent in glycoclusters. Despite their unique and diverse functionalities, investigations of metalloglycoclusters have just begun to make progress with the development of applications and methodologies related to biological fields. In this minireview, we describe the potential use of metalloglycoclusters as functional molecules particularly in the light of recent reports (including our reports) pertaining to their application for analyzing carbohydrate binding properties and for imaging living cells. A. IntroductionCarbohydrate molecules play an important role in a plethora of biological processes. Utilizing a characteristic molecular recognition property, a variety of functional carbohydrate molecules have been developed aimed at the discovery of new drugs, and the establishment or improvement of various clinical diagnostic methods. Glycoclusters represent one group of functional carbohydrate molecules which possess a clustered carbohydrate component. The generation of glycoclusters with elaborate structures can be achieved through rigorous organic synthesis. Some of the more wellknown glycoclusters are shown in Fig. 1, and their structures commonly involve a characteristic framework modified with carbohydrate components. For example, so-called "Sugar ball" glycoclusters comprise a dendrimer as the framework with each terminal of the branch possessing a carbohydrate moiety. The size of the ball can be controlled by the generation number (1-4). The distance between carbohydrate moieties of glycoclusters with a cyclodextrin framework can reportedly be varied by changing the ring size (5, 6). Gold nanoparticles (7-10), micelles or liposomes (11)

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Hexameric Supramolecular Scaffold Orients Carbohydrates To Sense Bacteria

Cited by 81 publications

References 72 publications

Avidity-controlled hydrogels for injectable co-delivery of induced pluripotent stem cell-derived endothelial cells and growth factors

Avidity-controlled hydrogels for injectable co-delivery of induced pluripotent stem cell-derived endothelial cells and growth factors

Fluorescently labelled glycans and their applications

Application of Functionalized Metalloglycoclusters

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