Hexanoic acid protects tomato plants against <i><scp>B</scp>otrytis cinerea</i> by priming defence responses and reducing oxidative stress

Finiti, Iván; Leyva, María de la O.; Vicedo, Begonya; Gómez-Pastor, Rocío; López-Cruz, Jaime; García‐Agustín, Pilar; Real, M. Dolores; González-Bosch, Carmen

doi:10.1111/mpp.12112

Cited by 66 publications

(87 citation statements)

References 67 publications

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“…In the current study, pathogen-induced accumulation of H 2 0 2 was delayed in the mutants compared to the WT backgrounds without affecting the basal resistance to B. cinerea, showing that H 2 O 2 is not involved in basal resistance. However, at an early infection stage, the oxidative burst has a central role in priming plant resistance against necrotrophic pathogens such as B. cinerea (Aranega Bou et al 2014;Finiti et al 2014). In the current study, the accumulation of H 2 O 2 at different time points post inoculation in the tomato leaves followed a process that was similar to that described in earlier works, i.e., H 2 O 2 generally accumulated until 14 h after infection and in most cases did not further accumulate at later evaluation times.…”

Section: Discussionmentioning

confidence: 99%

Induced systemic resistance in tomato (Solanum lycopersicum) against Botrytis cinerea by biochar amendment involves jasmonic acid signaling

et al. 2015

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Aims The goal of this study was to identify the induced resistance pathway mediated by biochar in the tomatoBotrytis cinerea pathosystem. Methods Tomato wild types and mutants modified in their salicylic acid (SA), ethylene (ET) or jasmonic acid (JA) metabolism were grown in a potting medium amended with biochar produced at 450ºC from greenhouse wastes, to identify the possible pathway(s) involved in biochar-mediated resistance to B. cinerea.Early cellular response of H 2 O 2 accumulation was biochemically tested, and the transcriptional changes of 12 defense-related genes upon B. cinerea challenge of detached leaflets were analyzed. Results Biochar amendment resulted in about 50 % reduction in B. cinerea disease severity in all tested genotypes with the exception of a JA deficient mutant, def1. Biochar amendment induced priming of early as well as late-acting defense responses particularly in the genes Pti5 (ET-related) and Pi2 (JA-related), which are known to be crucial in resistance against B. cinerea. Stronger and earlier H 2 O 2 accumulation subsequent to B. cinerea inoculation in all genotypes was observed as a result of biochar amendment, with the exception of the def1 mutation. Conclusion Biochar-mediated IR in the B. cinerea-tomato pathosystem involves the JA pathway.

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Section: Discussionmentioning

confidence: 99%

Induced systemic resistance in tomato (Solanum lycopersicum) against Botrytis cinerea by biochar amendment involves jasmonic acid signaling

et al. 2015

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“…Hexanoic acid IR has been studied on both the transcriptomic and metabolic levels in tomato in relation to its protective effect against B. cinerea [12,139]. A marked metabolic reprogramming was associated with resistance triggered by hexanoic acid where several citrate and other primary metabolites were down-regulated.…”

Section: Induced Resistance Mechanisms In Solanaceaementioning

confidence: 99%

Plant Resistance Inducers against Pathogens in Solanaceae Species—From Molecular Mechanisms to Field Application

Alexandersson

Mulugeta

Lankinen

et al. 2016

IJMS

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This review provides a current summary of plant resistance inducers (PRIs) that have been successfully used in the Solanaceae plant family to protect against pathogens by activating the plant’s own defence. Solanaceous species include many important crops such as potato and tomato. We also present findings regarding the molecular processes after application of PRIs, even if the number of such studies still remains limited in this plant family. In general, there is a lack of patterns regarding the efficiency of induced resistance (IR) both between and within solanaceous species. In many cases, a hypersensitivity-like reaction needs to form in order for the PRI to be efficient. “-Omics” studies have already given insight in the complexity of responses, and can explain some of the differences seen in efficacy of PRIs between and within species as well as towards different pathogens. Finally, examples of field applications of PRIs for solanaceous crops are presented and discussed. We predict that PRIs will play a role in future plant protection strategies in Solanaceae crops if they are combined with other means of disease control in different spatial and temporal combinations.

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“…These results suggested that ROS accumulation and LCD do favor tissue colonization by necrotrophs. Other reports, however, have shown that the relationship between HR induction, ROS build‐up and decreased resistance to necrotrophs is more intricate than was anticipated, and cannot be taken as a general rule (Malolepsza and Urbanek, ; Unger et al ., ; Finiti et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Reactive oxygen species generated in chloroplasts contribute to tobacco leaf infection by the necrotrophic fungus Botrytis cinerea

et al. 2017

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SUMMARYReactive oxygen species (ROS) play fundamental roles in plant responses to pathogen infection, including modulation of cell death processes and defense-related gene expression. Cell death triggered as part of the hypersensitive response enhances resistance to biotrophic pathogens, but favors the virulence of necrotrophs. Even though the involvement of ROS in the orchestration of defense responses is well established, the relative contribution of specific subcellular ROS sources to plant resistance against microorganisms with different pathogenesis strategies is not completely known. The aim of this work was to investigate the role of chloroplastic ROS in plant defense against a typical necrotrophic fungus, Botrytis cinerea. For this purpose, we used transgenic Nicotiana tabacum (tobacco) lines expressing a plastid-targeted cyanobacterial flavodoxin (pfld lines), which accumulate lower chloroplastic ROS in response to different stresses. Tissue damage and fungal growth were significantly reduced in infected leaves of pfld plants, as compared with infected wild-type (WT) counterparts. ROS build-up triggered by Botrytis infection and associated with chloroplasts was significantly decreased (70-80%) in pfld leaves relative to the wild type. Phytoalexin accumulation and expression of pathogenesis-related genes were induced to a lower degree in pfld plants than in WT siblings. The impact of fungal infection on photosynthetic activity was also lower in pfld leaves. The results indicate that chloroplast-generated ROS play a major role in lesion development during Botrytis infection. This work demonstrates that the modulation of chloroplastic ROS levels by the expression of a heterologous antioxidant protein can provide a significant degree of protection against a canonical necrotrophic fungus.

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Hexanoic acid protects tomato plants against Botrytis cinerea by priming defence responses and reducing oxidative stress

Cited by 66 publications

References 67 publications

Induced systemic resistance in tomato (Solanum lycopersicum) against Botrytis cinerea by biochar amendment involves jasmonic acid signaling

Induced systemic resistance in tomato (Solanum lycopersicum) against Botrytis cinerea by biochar amendment involves jasmonic acid signaling

Plant Resistance Inducers against Pathogens in Solanaceae Species—From Molecular Mechanisms to Field Application

Reactive oxygen species generated in chloroplasts contribute to tobacco leaf infection by the necrotrophic fungus Botrytis cinerea

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