Hexavalent Chromate Reductase Activity in Cell Free Extracts of<i>Penicillium</i>sp.

Arévalo-Rangel, Damaris L.; González, Juan Fernando Cárdenas; Juárez, Víctor Manuel Martínez; Rodríguez, Ismael Acosta

doi:10.1155/2013/909412

Cited by 32 publications

(18 citation statements)

References 41 publications

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“…Cr(VI) was first bound to the positively charged complexing groups on fungal surfaces, and then reduced to Cr(III) by adjacent electron-donor compounds (such as glutathione, cysteine, amino acid etc. ), or by chromate reductase excreted by the fungi [49][50][51]. In addition, it is essential to point out that a small proportion of Cr might also be absorbed by AMF as our previous study has confirmed the direct uptake and transport of Cr by ERM [20,21].…”

Section: Distribution and Speciation Of Cr In The Extraradical Mycelimentioning

confidence: 77%

Chromium immobilization by extra- and intraradical fungal structures of arbuscular mycorrhizal symbioses

Zhang

Sun

et al. 2016

Journal of Hazardous Materials

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Section: Distribution and Speciation Of Cr In The Extraradical Mycelimentioning

confidence: 77%

Chromium immobilization by extra- and intraradical fungal structures of arbuscular mycorrhizal symbioses

Zhang

Sun

et al. 2016

Journal of Hazardous Materials

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“…Among the electron donors tested, the reductase activity was found to increase upon supplementation of NADPH in the reaction mixtures ( Table 2) the oxidation of which donates an electron to the chromate reductase enzyme, which in turn was transferred to Cr(VI), converting it to the intermediate form, Cr(V), which might have accepted two electrons from other organic substances to produce Cr(III) [17].…”

Section: Resultsmentioning

confidence: 99%

Preliminary Characterization of a NADPH Dependent Chromate Reductase from Trichoderma pseudokoningii

2016

J Microbiol Microb Technol

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Intracellular chromate reductase enzyme extracted from a chromium tolerant strain Trichoderma pseudokoningii was partially purified by ultrafiltration and gel filtration chromatography. The purified fraction showed highest reducing activity in presence of 1% (w/v) hexavalent Chromium. NADPH at a conentration of 2 mM was found to act as electron donor in the reduction reaction. The intracellular chromate reductase was found to have an optimum pH and temperature of 7.4 and 70 °C respectively. The enhancement of reductase activity in presence of exogenous thiols indicated the presence of thiols at active site.

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“…This fungal strain, which was named SL2, was isolated by a method similar to that previously employed for isolating Cr(VI)-tolerant fungi from air contaminated with industrial vapors [ 20 ]. The potato dextrose agar (PDA) solid medium was used for microorganism enrichment, and consisted of 1 g of dextrose, 1.8 g of agar, and 100 mL of filtered soup of 20 g of potatoes boiled for 30 min.…”

Section: Experimental Methodsmentioning

confidence: 99%

Characterization of Penicillium oxalicum SL2 isolated from indoor air and its application to the removal of hexavalent chromium

Long

Liu

et al. 2018

PLoS ONE

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Removal of toxic Cr(VI) by microbial reduction is a promising approach to reducing its ecotoxicological impact. To develop bioremediation technologies, many studies have evaluated the application of microorganisms isolated from Cr(VI)-contaminated sites. Nonetheless, little attention has been given to microbes from the environments without a history of Cr(VI) contamination. In this study, we aimed to characterize the Cr(VI) tolerance and removal abilities of a filamentous fungus strain, SL2, isolated from indoor air. Based on phenotypic characterization and rDNA sequence analysis, SL2 was identified as Penicillium oxalicum, a species that has not been extensively studied regarding Cr(VI) tolerance and reduction abilities. SL2 showed high tolerance to Cr(VI) on solid and in liquid media, facilitating its application to Cr(VI)-contaminated environments. Growth curves of SL2 in the presence of 0, 100, 400, or 1000 mg/L Cr(VI) were well simulated by the modified Gompertz model. The relative maximal colony diameter and maximal growth rate decreased as Cr(VI) concentration increased, while the lag time increased. SL2 manifested remarkable efficacy of removing Cr(VI). Mass balance analysis indicated that SL2 removed Cr(VI) by reduction, and incorporated 0.79 mg of Cr per gram of dry biomass. In electroplating wastewater, the initial rate of Cr(VI) removal was affected by the initial contaminant concentration. In conclusion, P. oxalicum SL2 represents a promising new candidate for Cr(VI) removal. Our results significantly expand the knowledge on potential application of this microorganism.

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Hexavalent Chromate Reductase Activity in Cell Free Extracts ofPenicilliumsp.

Cited by 32 publications

References 41 publications

Chromium immobilization by extra- and intraradical fungal structures of arbuscular mycorrhizal symbioses

Chromium immobilization by extra- and intraradical fungal structures of arbuscular mycorrhizal symbioses

Preliminary Characterization of a NADPH Dependent Chromate Reductase from Trichoderma pseudokoningii

Characterization of Penicillium oxalicum SL2 isolated from indoor air and its application to the removal of hexavalent chromium

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