Hg2+-selective “turn-on” fluorescent chemodosimeter derived from glycine and living cell imaging

Mahapatra, Ajit Kumar; Roy, Jagannath; Kundu, Supratim; Sahoo, Prithidipa; Mukhopadhyay, Subhra Kanti; Banik, Avishek

doi:10.1016/j.jphotochem.2012.05.001

Cited by 17 publications

(3 citation statements)

References 39 publications

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“…However, these metals are toxic at very high concentration for example, copper at higher concentration decreases the photosystem II (PSII) efficiency and causes oxidative damage (Chandrashekharaiah, Sanyal, Dasgupta, Sapre, & Banik, 2020). The non‐essential heavy metals (cadmium, lead, and mercury) do not have any known biological role and are toxic at very low concentrations (Mahapatra et al, 2012). Non‐essential heavy metals exert adverse effects by inactivating photosynthetic machinery, enzymatic pathway, growth, and morphology of algae (Chandrashekharaiah, Sanyal, Dasgupta, Sapre, & Banik, 2020).…”

Section: Introductionmentioning

confidence: 99%

Phycoremediation and photosynthetic toxicity assessment of lead by two freshwater microalgae Scenedesmus acutus and Chlorella pyrenoidosa

Shivagangaiah

Sanyal

Dasgupta

et al. 2021

Physiologia Plantarum

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Heavy metal (HM) pollution is a serious agro‐economic concern and algae can be used as one of the bioremediating agents as it can grow in different water bodies. In this study, the Scenedesmus acutus and Chlorella pyrenoidosa were exposed to various concentrations of Pb2+ for 96 h and a multidimensional toxicity assessment has been performed by pulse amplitude modulation technique and Fourier transform infrared spectroscopy (FTIR). High‐angle annular dark‐field scanning transmission electron microscopy coupled energy dispersive spectroscopy (HAADF‐S/TEM‐EDS) detected intracellular localization of Pb2+, thus confirming algal bio‐accumulation abilities. Sensitivity assay demonstrated that 500 and 400 ppm of Pb2+ as minimum inhibitory concentrations (MIC50) for S. acutus and C. pyrenoidosa, respectively, which inhibited growth (OD) by >50% in 96 h. During bioremoval studies, S. acutus and C. pyrenoidosa were found to remove ∼52 and ∼32% of total Pb2+, respectively. The particulate analysis of Pb2+ by ICP‐OES showed >99.5% biosorption capacity by both the species. The biomass characterization by FTIR showed the involvement of various cell wall functional groups such as hydroxyl, alkane, and C=C groups in the biosorption of Pb2+ by both the species. The noninvasive chlorophyll fluorescence techniques provide a quick insight on heavy metal stress and can be adapted as a rapid detection tool to study the Pb2+ stress. S. acutus strain showed higher tolerance and higher bioremoval capacity than C. pyrenoidosa. However, both the species can be exploited for biosorption of Pb2+ from aquatic streams as an alternative way for low cost Pb2+recovery systems.

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Section: Introductionmentioning

confidence: 99%

Phycoremediation and photosynthetic toxicity assessment of lead by two freshwater microalgae Scenedesmus acutus and Chlorella pyrenoidosa

Shivagangaiah

Sanyal

Dasgupta

et al. 2021

Physiologia Plantarum

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“…[13][14][15][16][17] In this regard, mercury has been regarded as one of the most toxic metals and it exists in a variety of forms, though mainly as Hg 2+ . [17][18][19] Mercury contamination occurs through a variety of natural and anthropogenic sources and Hg 2+ has caused serious problems for human health and ecology. [20][21][22][23] The Environmental Protection Agency (EPA) standard for the maximum allowable level of inorganic Hg 2+ in drinking water is 2.0 ppb.…”

Section: Introductionmentioning

confidence: 99%

“…Because Hg 2+ is a heavy metal ion that always causes quenching of the fluorescence signal, few articles have reported the turn-on or ratiometric detection of Hg 2+ . 11,18,19,24 To assist in the detection of Hg 2+ in environmental and biological fields, probes should have good water solubility and biological compatibility. [25][26][27] In this respect, the conjugation of a fluorophore with amino acids is a promising strategy for the synthesis of new fluorescent probes, as amino acids are water-soluble and biologically compatible.…”

Section: Introductionmentioning

confidence: 99%

Selective and sensitive fluorescence-shift probes based on two dansyl groups for mercury(ii) ion detection

Liu

Deng

et al. 2014

Photochem Photobiol Sci

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Two probes ( and ) bearing two dansyl fluorophores were synthesized and applied to the detection of mercury(ii) ions in aqueous solution. These probes exhibited a selective response to Hg(2+) in a buffered solution, with high sensitivity and a unique fluorescence response signal which displayed a blue-shift effect in the fluorescence emission peak. The Hg(2+) recognition mechanisms of the probes were determined by NMR spectroscopy, ESI-MS and UV-vis spectroscopy. The results showed that probe and mercury(ii) ions formed an unusual 2:2 stoichiometric ratio complex, while probe and Hg(2+) formed a multidentate complex with a stoichiometric ratio of 2:1.

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Fluorescence resonance energy transfer (FRET)-based technique for tracking of endophytic bacteria in rice roots

et al. 2015

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Hg2+-selective “turn-on” fluorescent chemodosimeter derived from glycine and living cell imaging

Cited by 17 publications

References 39 publications

Phycoremediation and photosynthetic toxicity assessment of lead by two freshwater microalgae Scenedesmus acutus and Chlorella pyrenoidosa

Phycoremediation and photosynthetic toxicity assessment of lead by two freshwater microalgae Scenedesmus acutus and Chlorella pyrenoidosa

Selective and sensitive fluorescence-shift probes based on two dansyl groups for mercury(ii) ion detection

Fluorescence resonance energy transfer (FRET)-based technique for tracking of endophytic bacteria in rice roots

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