hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2

Saraiva, Nuno; Prole, David L.; Carrara, Guia; Johnson, Benjamin F.; Taylor, Colin W.; Parsons, Maddy; Smith, Geoffrey L.

doi:10.1083/jcb.201301016

Cited by 62 publications

(71 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A recent study demonstrated the role of calpain 2 in mediating the effects of Golgi antiapoptotic proteins (GAAPs), which promote Ca 2+ release from intracellular stores, on cell motility. Inhibition or knockdown of calpain 2 attenuated the effects of GAAP on cell spreading and migration (Saraiva, 2013). Clearly, the calpain system has vital physiological implications, which remain to be elucidated; however, the advancement of molecular approaches will undoubtedly provide further advances in our understanding of the many physiological roles of calpain.…”

Section: Calpain In Cell Motilitymentioning

confidence: 99%

Calcium and Proteases

Schnellmann

2018

Comprehensive Toxicology

View full text Add to dashboard Cite

Section: Calpain In Cell Motilitymentioning

confidence: 99%

Calcium and Proteases

Schnellmann

2018

Comprehensive Toxicology

View full text Add to dashboard Cite

“…For example, subcellular elevation of cytosolic free Ca 2+ by SOCE is required for renal epithelial cell, osteoclast, endothelial cell, and breast cancer cell migration (Fabian et al, ; Yang et al, ; Wheal et al, ; Tsai et al, ). Stimulation of SOCE‐mediated calpain 2 activation increases cell adhesion, spreading, and migration in ovary cells as well as cervical and osteosarcoma cancer cells (Huttenlocher et al, ; Saraiva et al, ). SOCE activation increases the actomyosin contractile force, thus, controlling the migration of cervical cancer cells (Vicente‐Manzanares et al, ; Chen et al, ).…”

mentioning

confidence: 99%

Mechanosensitive store‐operated calcium entry regulates the formation of cell polarity

Huang

Chang

Harn

et al. 2015

Journal Cellular Physiology

View full text Add to dashboard Cite

Ca(2+) -mediated formation of cell polarity is essential for directional migration which plays an important role in physiological and pathological processes in organisms. To examine the critical role of store-operated Ca(2+) entry, which is the major form of extracellular Ca(2+) influx in non-excitable cells, in the formation of cell polarity, we employed human bone osteosarcoma U2OS cells, which exhibit distinct morphological polarity during directional migration. Our analyses showed that Ca(2+) was concentrated at the rear end of cells and that extracellular Ca(2+) influx was important for cell polarization. Inhibition of store-operated Ca(2+) entry using specific inhibitors disrupted the formation of cell polarity in a dose-dependent manner. Moreover, the channelosomal components caveolin-1, TRPC1, and Orai1 were concentrated at the rear end of polarized cells. Knockdown of TRPC1 or a TRPC inhibitor, but not knockdown of Orai1, reduced cell polarization. Furthermore, disruption of lipid rafts or overexpression of caveolin-1 contributed to the downregulation of cell polarity. On the other hand, we also found that cell polarity, store-operated Ca(2+) entry activity, and cell stiffness were markedly decreased by low substrate rigidity, which may be caused by the disorganization of actin filaments and microtubules that occurs while regulating the activity of the mechanosensitive TRPC1 channel.

show abstract

“…Through modulation of Ca 2+ homeostasis and Ca 2+ signaling, TMBIM4 can mediate cell adhesion and migration [57,58]. TMBIM4 suppresses cell death of both intrinsic pathways induced by ceramide and H 2 O 2 , and extrinsic pathways induced by Fas ligand and TNF.…”

Section: Tmbim Proteins In Mediating Cell Death and Ca2+ Homeostasismentioning

confidence: 99%

TMBIM-mediated Ca2+ homeostasis and cell death

Liu

2017

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

View full text Add to dashboard Cite

Ca2+ is a ubiquitous intracellular messenger that regulates numerous physiological activities in humans, animals, plants, and bacteria. Cytosolic Ca2+ is kept at a low level, but subcellular organelles such as the endoplasmic reticulum (ER) and Golgi apparatus maintain high-concentration Ca2+ stores. Under resting conditions, store Ca2+ homeostasis is dynamically regulated to equilibrate between active Ca2+ uptake and passive Ca2+ leak processes. The evolutionarily conserved Transmembrane BAX Inhibitor-1 Motif-containing (TMBIM) proteins mediate Ca2+ homeostasis and cell death. This review focuses on recent advances in functional and structural analysis of TMBIM proteins in regulation of the two related functions. The roles of TMBIM proteins in pathogen infection and cancer are also discussed with prospects for treatment.

show abstract

hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2

Abstract: hGAAP promotes cell adhesion and migration by increasing localized Ca2+-dependent activation of calpain, leading to increased focal adhesion dynamics.

Cited by 62 publications

References 61 publications

Calcium and Proteases

Calcium and Proteases

Mechanosensitive store‐operated calcium entry regulates the formation of cell polarity

TMBIM-mediated Ca2+ homeostasis and cell death

Contact Info

Product

Resources

About