Hibiscus sabdariffa leaf induces apoptosis of human prostate cancer cells in vitro and in vivo

Lin, Hui-Hsuan; Chan, Kuei-Chuan; Sheu, Jenn-Yuan; Hsuan, Shu-Wen; Wang, Chau‐Jong; Chen, Jing-Hsien

doi:10.1016/j.foodchem.2011.11.057

Cited by 70 publications

(41 citation statements)

References 31 publications

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“…An ethanol extract of the leaves of H. sabdariffa was shown to retard aflatoxin formation (Müller and Franz ), hypolipidemic (Traganos and Darzynkiewicz ), anti‐oxidant and anti‐hyperlipidemic activity (Ochani and D'Mello ), and against Aspergillus fumigatus, Rhizopus nigricans , and Trichophyton mentagrophytes (Guerin and Reveillere ). Our previous studies have also exhibited that the aqueous extract of Hibiscus leaf had high quantity of polyphenol and induced apoptosis in prostate cancer cells (Lin and others ). Regarding the effect of Hibiscus leaf in vitro and in vivo studies, the effect of the polyphenolic extract in cancer is rarely reported.…”

Section: Discussionmentioning

confidence: 82%

“…Previous studies had demonstrated Hibiscus leaves possessed hypolipidemic, antioxidant and oestrogenic‐like effects (Ali and others ; Kuriyan and others ). Our recent studies have also found that an extract of Hibiscus leaves possess anticancer (Lin and others ) and anti‐atherosclerotic effects (Chen and others ). Nevertheless, there are few studies on the characterization of the polyphenolic extract of Hibiscus leaves.…”

Section: Introductionmentioning

confidence: 78%

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Hibiscus sabdariffa Leaf Polyphenolic Extract Induces Human Melanoma Cell Death, Apoptosis, and Autophagy

Chiu

Hsuan

Lin

et al. 2015

Journal of Food Science

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Melanoma is the least common but most fatal form of skin cancer. Previous studies have indicated that an aqueous extract of Hibiscus sabdariffa leaves possess hypoglycemic, hypolipidemic, and antioxidant effects. In this study, we want to investigate the anticancer activity of Hibiscus leaf polyphenolic (HLP) extract in melanoma cells. First, HLP was exhibited to be rich in epicatechin gallate (ECG) and other polyphenols. Apoptotic and autophagic activities of HLP and ECG were further evaluated by DAPI stain, cell-cycle analysis, and acidic vascular organelle (AVO) stain. Our results revealed that both HLP and ECG induced the caspases cleavages, Bcl-2 family proteins regulation, and Fas/FasL activation in A375 cells. In addition, we also revealed that the cells presented AVO-positive after HLP treatments. HLP could increase the expressions of autophagy-related proteins autophagy-related gene 5 (ATG5), Beclin1, and light chain 3-II (LC3-II), and induce autophagic cell death in A375 cells. These data indicated that the anticancer effect of HLP, partly contributed by ECG, in A375 cells. HLP potentially could be developed as an antimelanoma agent.

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Section: Discussionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 78%

Hibiscus sabdariffa Leaf Polyphenolic Extract Induces Human Melanoma Cell Death, Apoptosis, and Autophagy

Chiu

Hsuan

Lin

et al. 2015

Journal of Food Science

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“…The animals were randomly divided into three groups (twelve mice per group) and treated as follows: (i) saline (vehicle); (ii) LNCaP alone; (iii) LNCaP + GTIN: GTIN at 30 mg/kg of body weight once daily, p.o. The injection regiments and doses for the treatments were based on the published reports . The day of cell implantation was designated as day 0, and tumor growth, and tumor appearance were registered daily thereafter.…”

Section: Methodsmentioning

confidence: 99%

“…The injection regiments and doses for the treatments were based on the published reports. 32,33 The day of cell implantation was designated as day 0, and tumor growth, and tumor appearance were registered daily thereafter. The animals were euthanized, and the primary tumors were removed and measured 42 days later.…”

Section: Xenograft Tumor Studiesmentioning

confidence: 99%

Anti‐prostate cancer potential of gossypetin via inducing apoptotic and autophagic cell death

Lee

Tsai

Wang

et al. 2017

Molecular Carcinogenesis

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Gossypetin (GTIN), a naturally occurring hexahydroxy flavone, has been shown to possess antimutagenic, antioxidant, antimicrobial, and antiatherosclerotic effects. Here, we investigated the mechanism(s) underlying the anticancer potential of GTIN. In this study, investigations were showed that GTIN preferentially induces programed cell death of prostate cancer (PCa) cells in vitro and in vivo. MTT data showed that GTIN exhibited the anti-proliferation effect on human PCa cells in a dose- and time-dependent manner. Among two kinds of PCa cells, androgen-dependent LNCaP cells were the most susceptible to GTIN. GTIN was evaluated for apoptotic and autophagic activities in LNCaP cells, but not in androgen-independent DU145 cells with mutant Atg5 and resistant to autophagy. Molecular data showed the apoptotic effect of GTIN at a high dose in PCa cells might be mediated via mitochondrial pathway. The lower dose of GTIN-induced autophagy enhances LNCaP cell death, and is dependent on class III PI3K and Atg5 pathway. Finally, GTIN was evidenced by its inhibition on the growth of LNCaP cells in xenograft tumor studies. As a result, our data presented the first evidence of GTIN as an inducer of apoptotic and autophagic cell death in LNCaP cells, and provide a new mechanism for its anticancer activity.

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“…In India, Africa and Mexico, infusions of the leaves or calyces are traditionally used for their diuretic, cholerectic, febrifugal and hypotensive effects (Kuo et al, 2012;Guardiola and Mach, 2014). Research has shown that H. sabdariffa L. leaves have multiple biological activities, such as antiatherosclerotic effect (Chen et al, 2013), anticancer activity (Lin et al, 2012), antioxidant and antihyperlipidemic activities (Ochani and D'Mello, 2009;Gosain et al, 2010;Sindi et al, 2014). Flavonoids and phenolic acids are considered as the major bioactive compounds in the leaves of H. sabdariffa L. (Chen et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Variations in chemical fingerprints and major flavonoid contents from the leaves of thirty‐one accessions of Hibiscus sabdariffa L.

Jin

Cao

Ferchaud

et al. 2015

Biomedical Chromatography

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The leaves of Hibiscus sabdariffa L. have been used as traditional folk medicines for treating high blood pressure and fever. There are many accessions of H. sabdariffa L. throughout the world. To assess the chemical variations of 31 different accessions of H. sabdariffa L., fingerprinting analysis and quantitation of major flavonoids were performed by high‐performance liquid chromatography (HPLC). The HPLC method was validated for linearity, sensitivity, precision, repeatability and accuracy. A quadrupole‐time‐of‐flight mass spectrometry (Q‐TOF‐MS) was applied for the characterization of major compounds. A total of 9 compounds were identified, including 6 flavonoids and 3 phenolic acids. In the fingerprint analysis, similarity analysis (SA) and principal component analysis (PCA) were used to differentiate the 31 accessions of H. sabdariffa L. Based on the results of PCA and SA, the samples No. 15 and 19 appeared much different from the main group. The total content of five flavonoids varied greatly among different accessions, ranging from 3.35 to 23.30 mg/g. Rutin was found to be the dominant compound and the content of rutin could contribute to chemical variations among different accessions. This study was helpful to understand the chemical variations between different accessions of H. sabdariffa L., which could be used for quality control. © 2015 The Authors Biomedical Chromatography Published by John Wiley & Sons Ltd.

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Hibiscus sabdariffa leaf induces apoptosis of human prostate cancer cells in vitro and in vivo

Cited by 70 publications

References 31 publications

Hibiscus sabdariffa Leaf Polyphenolic Extract Induces Human Melanoma Cell Death, Apoptosis, and Autophagy

Hibiscus sabdariffa Leaf Polyphenolic Extract Induces Human Melanoma Cell Death, Apoptosis, and Autophagy

Anti‐prostate cancer potential of gossypetin via inducing apoptotic and autophagic cell death

Variations in chemical fingerprints and major flavonoid contents from the leaves of thirty‐one accessions of Hibiscus sabdariffa L.

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