Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression

Xu, Xing; Choi, Han Sol; Park, Sang Yong; Kim, Jinkyu; Seo, Kwang; Kim, Hoon; Kim, Yeon-Ju

doi:10.1016/j.phymed.2021.153870

Cited by 10 publications

(9 citation statements)

References 31 publications

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“…After T + I stimulation for 24 h, the cells were rinsed twice with phosphate-buffered saline (PBS; pH 7.2). Total RNA extraction, reverse transcription of RNA into cDNA, and quantitative real-time PCR (qRT-PCR) were performed as described previously . The sequences of the gene-specific primers (Macrogen, Seoul, Korea) are listed in Table S1.…”

Section: Methodsmentioning

confidence: 99%

“…The cells were rinsed twice with PBS and extracted using RIPA lysis buffer (Thermo Fisher Scientific, Waltham, MA, USA) containing protease inhibitors (GenDEPOT, Katy, TX, USA). The collected proteins were normalized using a bicinchoninic acid protein assay kit (Thermo Fisher Scientific), separated on a 10% sodium dodecyl sulfate-polyacrylamide gel, and transferred to a polyvinylidene fluoride membrane (Thermo Fisher Scientific) according to a previously described method . Western blotting was used to evaluate NF-κB and MAPK signaling molecules as previously reported .…”

Section: Methodsmentioning

confidence: 99%

“…The collected proteins were normalized using a bicinchoninic acid protein assay kit (Thermo Fisher Scientific), separated on a 10% sodium dodecyl sulfate-polyacrylamide gel, and transferred to a polyvinylidene fluoride membrane (Thermo Fisher Scientific) according to a previously described method. 50 Western blotting was used to evaluate NF-κB and MAPK signaling molecules as previously reported. 51 The antibodies used in this study are listed in Table S3 , which were detected using a chemiluminescent imaging system (WSE-6370 LuminoGraph III Lite; ATTA, Tokyo, Japan) and quantified using ImageJ software ( ; NIH, Bethesda, MD, USA).…”

Section: Methodsmentioning

confidence: 99%

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Biologically Synthesized Rosa rugosa-Based Gold Nanoparticles Suppress Skin Inflammatory Responses via MAPK and NF-κB Signaling Pathway in TNF-α/IFN-γ-Induced HaCaT Keratinocytes

et al. 2022

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Nanotechnology-applied materials and related therapeutics have gained attention for treating inflammatory skin diseases. The beach rose (Rosa rugosa), belonging to the family Rosaceae, is a perennial, deciduous woody shrub endemic to northeastern Asia. In this study, R. rugosa-based gold nanoparticles (RR-AuNPs) were biologically synthesized under optimal conditions to explore their potential as anti-inflammatory agents for treating skin inflammation. The synthesized RR-AuNPs were analyzed using field emission-transmission electron microscopy, energy-dispersive X-ray spectrometry, selected-area electron diffraction, and X-ray diffraction. The uniformly well-structured AuNPs showed near-spherical and polygonal shapes. Cell viability evaluation and optical observation results showed that the RR-AuNPs were absorbed by human keratinocytes without causing cytotoxic effects. The effects of RR-AuNPs on the skin inflammatory response were investigated in human keratinocytes treated with tumor necrosis factor-α/interferon-γ (T + I). The results showed that T + I-stimulated increases in inflammatory mediators, including chemokines, interleukins, and reactive oxygen species, were significantly suppressed by RR-AuNP treatment in a concentration-dependent manner. The western blotting results indicated that the RR-AuNP-mediated anti-inflammatory effects were highly associated with the suppression of inflammatory signaling, mitogen-activated protein kinase, and nuclear factor-κB. These results demonstrate that plant extract-based AuNPs are novel anti-inflammatory candidates for topical application to treat skin inflammation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Biologically Synthesized Rosa rugosa-Based Gold Nanoparticles Suppress Skin Inflammatory Responses via MAPK and NF-κB Signaling Pathway in TNF-α/IFN-γ-Induced HaCaT Keratinocytes

et al. 2022

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“…The suppressive effect of phytochemicals on cholesterol biosynthesis has been suggested in a colorectal cancer model. The callus extract of Hibiscus syriacus L., which is rich in fumaric acid and denatonium benzoate, was found to mediate Notch signaling, which subsequently suppressed cholesterol synthesis . Moreover, the proteins related to cholesterol biosynthesis, including HMGCR, FDFT1, squalene epoxidase (SQLE), and lanosterol synthase (LSS), were downregulated due to a reduction in Notch signaling and the activation of AMPK signaling.…”

Section: Regulation Of Cholesterol Homeostasismentioning

confidence: 99%

Exploring Phytochemical Mechanisms in the Prevention of Cholesterol Dysregulation: A Review

Weerawatanakorn,

Kamchonemenukool,

Koh

et al. 2024

J. Agric. Food Chem.

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Although cholesterol plays a key role in many physiological processes, its dysregulation can lead to several metabolic diseases. Statins are a group of drugs widely used to lower cholesterol levels and cardiovascular risk but may lead to several side effects in some patients. Therefore, the development of a plant-based therapeutic adjuvant with cholesterol-lowering activity is desirable. The maintenance of cholesterol homeostasis encompasses multiple steps, including biosynthesis and metabolism, uptake and transport, and bile acid metabolism; issues arising in any of these processes could contribute to the etiology of cholesterolrelated diseases. An increasing body of evidence strongly indicates the benefits of phytochemicals for cholesterol regulation; traditional Chinese medicines prove beneficial in some disease models, although more scientific investigations are needed to confirm their effectiveness. One of the main functions of cholesterol is bile acid biosynthesis, where most bile acids are recycled back to the liver. The composition of bile acid is partly modulated by gut microbes and could be harmful to the liver. In this regard, the reshaping effect of phytochemicals on gut microbiota has been widely reported in the literature for its significance. Therefore, we reviewed studies conducted over the past 5 years elucidating the regulatory effects of phytochemicals or herbal medicines on cholesterol metabolism. In addition, their effects on the recomposition of gut microbiota and bile acid metabolism due to modulation are discussed. This review aims to provide novel insights into the treatment of cholesterol dysregulation and the anticipated development of natural-based compounds in the near and far future.

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“…Recently, some pharmaceutical effects of H. syriacus have been reported; therefore, its value has increased not only as a gardening plant but also as an essential ingredient in medicine. − …”

Section: Introductionmentioning

confidence: 99%

Identification and Field Bioassay of Rusicada privata (Lepidoptera: Erebidae) Sex Pheromone

Kim

Lee

2023

J. Agric. Food Chem.

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Rusicada privata (Lepidoptera: Erebidae) is a major pest insect on Hibiscus syriacus L. (Malvaceae: Malvales), which is usually planted in urban landscapes. Insecticidal control of R. privata is not ideal for urban landscaping due to its harmful effects and risk to human health. Therefore, nonchemical and ecofriendly alternatives are needed. To identify the sex pheromone of R. privata, abdominal tip extracts of male and female R. privata were analyzed by gas chromatography–mass spectrometry. The abundance of 7-methylheptadecane (7Me-17Hy) in female R. privata abdominal tip extracts led us to hypothesize that it is the major sex pheromone. The compound was tentatively identified by a mass spectral library and confirmed by matching retention times and mass spectra of the female-produced compound with those of a synthetic standard. The compounds elicited electroantennographic (EAG) activity. In a field trapping experiment, only synthetic lures containing 7Me-17Hy attracted R. privata males. The EAG activity and field trapping results confirmed that 7Me-17Hy is the sex pheromone of female R. privata. The results will aid in developing sex pheromone-based R. privata control techniques, such as mating disruption.

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Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression

Cited by 10 publications

References 31 publications

Biologically Synthesized Rosa rugosa-Based Gold Nanoparticles Suppress Skin Inflammatory Responses via MAPK and NF-κB Signaling Pathway in TNF-α/IFN-γ-Induced HaCaT Keratinocytes

Biologically Synthesized Rosa rugosa-Based Gold Nanoparticles Suppress Skin Inflammatory Responses via MAPK and NF-κB Signaling Pathway in TNF-α/IFN-γ-Induced HaCaT Keratinocytes

Exploring Phytochemical Mechanisms in the Prevention of Cholesterol Dysregulation: A Review

Identification and Field Bioassay of Rusicada privata (Lepidoptera: Erebidae) Sex Pheromone

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