Hide Dehairing and Laundry Detergent Compatibility Testing of Thermostable and Solvents Tolerant Alkaline Protease from Hot Spring Isolate <i>Bacillus cohnii</i>U3

Abstract

Abstract:In the present study, alkaline protease producing thermophilic Bacillus cohniiU3 strain was isolation from Unnai hot spring, India. The maximum production of protease (344 U/mL) was reported after 72 h of inoculation at 50°C in shake flask culture using the gelatin casein medium. The protease was found to remain active up to 96 h and production was growth dependent. The activity of parital purified protease was reported in abroad range of pH ranging from 4.0 to 11.0 with an optimum of 9.0 pH; Enzymes was indicated the highest activity at 50°C temperature. Salt independent catalysis and activity with a broad range of substrate concentration is the key feature of the protease. Thermos table nature, the stability in alkaline pH and stability in high salt concentration for 45 min were outstanding features of protease. The enzyme was stable in the presence of various organic solvents like Dimethyl Sulphoxide (DMSO), Methanol, Butanol, n-Hexane, Benzene at 25% (v/v) concentration. A good compatibility of the enzyme with most commercial detergents indicated its application in detergent industry. The remarkable dehairing in goat hide and destaining of blood spot in 2 h using 10 U/mL of protease assure that it could be a potential candidate for leather and detergent industries.

Isolation and identification of protease‐producing bacteria from sludge and sediment soil around Adama, Ethiopia

“…Dehairing activity was performed by the method described by (Dudhgara et al 2015). Sheepskin was cut 3 × 2 cm pieces and washed gently with tap water and then rinsed with distilled water to remove chemicals from the skin, which may hinder enzyme activity during dehairing ac tivity.…”

Thermotolerant bacteria of biotechnological potential from hot springs in Eritrea

“…Thermotolerant bacteria have attracted industrial and biotechnological attention as their enzymes are well suited for harsh industrial processes (Abdel-Rahman et al, 2016;Archna et al, 2015;Verma et al, 2015). Proteases obtained from thermophilic or thermotolerant (Adhikari et al, 2015;Bozoglu et al, 2015;Lele and Deshmukh, 2016;Panda et al, 2013;Remigio et al, 2012;Verma et al, 2014) bacteria, for example, have found applications such as hide dehairing in the leather industry and stain removal in laundry detergents (Chandrashekhar and Narayan, 2015;Dudhgara et al, 2015;Jaouadi et al, 2013). Cellulases have showed great potential in the production of bioethanol and other speciality chemicals from renewable agricultural residues (Hardiman et al, 2010).…”