Hierarchical analysis of genetic structure in Spanish donkey breeds using microsatellite markers

Abstract

The hierarchical population structure of five, native-Spanish donkey breeds (Andaluza, Catalana, Mallorquina, Encartaciones and Zamorano-Leonesa) has been studied using Fstatistics. In addition, nine Moroccan asses and 24 Merens breed horses were included in the analysis. Data came from 15 DNA microsatellites. The analysis shows that Spanish donkeys are substructured at both hierarchical levels studied, among breeds and within breeds (between subpopulations). In the whole population, the deficit of heterozygotes was estimated to be about 21%. The fixation

High polymorphism at microsatellite loci in the Chinese donkey

“…In contrast, the locus HMS1 was found to be monomorphic in both Chinese and Spanish donkeys (Aranguren-Méndez et al, 2002). Generally, the Chinese donkey shows relatively higher genetic diversity than the Spanish donkey at the seven polymorphic loci investigated in the current study.…”

“…Among the 10 microsatellites investigated in this study, the PIC of HTG4 and HMS5 were found to be 0.40 and 0.53 in five endangered Spanish donkey breeds, respectively, while both were monomorphic in Chinese donkey breeds (Aranguren-Méndez et al, 2002). In contrast, the locus HMS1 was found to be monomorphic in both Chinese and Spanish donkeys (Aranguren-Méndez et al, 2002).…”

“…Jordana et al (2001) found that microsatellite loci are effective at revealing genetic variation among donkey breeds in the Catalonian region. Using 15 microsatellite markers to analyze the hierarchical genetic structure of five endangered Spanish donkey breeds, Aranguren-Méndez et al (2002) revealed that four breeds with black coats from northern Spain form a closed cluster and supported the hypothesis of a common ancestral past from Equus asinus europeus. Using eight microsatellites, Ivankovic et al (2002) revealed relatively high levels of heterozygosity in three donkey populations in the coastal region of Croatia.…”

“…These 10 microsatellites have been shown to be effective markers in the study of genetic diversity of donkeys in previous studies (Jordana et al, 2001;Aranguren-Méndez et al, 2002;Ivankovic et al, 2002;Xie, 2004;Zhu and Su, 2011).…”

Evaluation of genetic diversity and population structure of five Chinese indigenous donkey breeds using microsatellite markers

“…To date, the genetic diversity of indigenous donkey breeds in Spain (Aranguren-Mendez et al 2001;Aranguren-Mendez et al 2002), Croatia (Ivankovic et al 2002), Italy (Bordonaro et al 2012;Colli et al 2013;Matassino et al 2014), America (Jordana et al 2015), northeast Africa, the Near East and the Arabian Peninsula (Rosenbom et al 2015) has been evaluated using microsatellite markers, and these results of evaluation are crucial for making further conservation strategy. On the other hand, the origin of the species and evolutionary relationship between donkey breeds were analyzed using the mitochondrial DNA markers (Ivankovic et al 2002;Lopez Lopez et al 2005;Chen et al 2006;Zhang et al 2010;Han et al 2014;Perez-Pardal et al 2014), and these results hinted that the modern donkey (including Chinese donkey) has an African maternal origin.…”