Hierarchical Self‐Assembly of One‐Dimensional Streptavidin Bundles as a Collagen Mimetic for the Biomineralization of Calcite

Burazerovic, Sabina; Gradinaru, Julieta; Pierron, Julien; Ward, Thomas R.

doi:10.1002/anie.200701080

Cited by 117 publications

(76 citation statements)

References 82 publications

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“…However, this task is complicated by the fact that proteins are complex macromolecules that do not possess many canonical interaction motifs with which to program their organization. A number of strategies have been devised toward this end, including computational design of associative surfaces (8), the construction of chimeric protein assemblies with preprogrammed symmetries (9,10), and those that use specific ligand-protein interactions (11)(12)(13)(14), metal coordination (15)(16)(17), disulfide bonds (18)(19)(20), or a combination of these strategies (21,22) to connect protein building blocks. These approaches have produced supramolecular architectures with increasing structural sophistication or improved functions.…”

mentioning

confidence: 99%

Exceptionally stable, redox-active supramolecular protein assemblies with emergent properties

Brodin

Carr

Sontz

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

105

107

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The designed assembly of proteins into well-defined supramolecular architectures not only tests our understanding of proteinprotein interactions, but it also provides an opportunity to tailor materials with new physical and chemical properties. Previously, we described that RIDC3, a designed variant of the monomeric electron transfer protein cytochrome cb 562 , could self-assemble through Zn 2+ coordination into uniform 1D nanotubes or 2D arrays with crystalline order. Here we show that these 1D and 2D RIDC3 assemblies display very high chemical stabilities owing to their metal-mediated frameworks, maintaining their structural order in ≥90% (vol/vol) of several polar organic solvents including tetrahydrofuran (THF) and isopropanol (iPrOH). In contrast, the unassembled RIDC3 monomers denature in ∼30% THF and 50% iPrOH, indicating that metal-mediated self-assembly also leads to considerable stabilization of the individual building blocks. The 1D and 2D RIDC3 assemblies are highly thermostable as well, remaining intact at up to ∼70°C and ∼90°C, respectively. The 1D nanotubes cleanly convert into the 2D arrays on heating above 70°C, a rare example of a thermal crystalline-to-crystalline conversion in a biomolecular assembly. Finally, we demonstrate that the Zn-directed RIDC3 assemblies can be used to spatiotemporally control the templated growth of small Pt 0 nanocrystals. This emergent function is enabled by and absolutely dependent on both the supramolecular assembly of RIDC3 molecules (to form a periodically organized structural template) and their innate redox activities (to direct Pt 2+ reduction).protein self-assembly | supramolecular coordination chemistry | nanomaterials | biomaterials | inorganic nanoparticles T he enormous structural and chemical diversity of proteins makes them highly attractive building blocks for functional materials. Supramolecular protein assemblies constitute the major components of cellular machinery, and many of them [e.g., 0D ferritin (1), 1D silicatein filaments (2, 3), 2D S-layers (4)] have also found a number of applications in nano-and biotechnology (2, 4, 5). Such protein assemblies are typically highly ordered, yet they possess dynamic structures that respond to external stimuli (6). Distinctively, protein assemblies are innately functional: the individual building blocks of these assemblies often perform specific chemical functions. For instance, the monomeric components of silicatein filaments or ferritin cages each have catalytic sites that process the precursors (SiO 2 or Fe 2+ ) for the downstream biomineralization processes (1, 3). On self-assembly, these individual components can be significantly stabilized (allowing them to withstand the extracellular environment and perform their functions) (7), and their chemical function takes on an entirely different context within the supramolecular architecture (acting as the template for biomineralization in the cases of silicatein and ferritin). Thus, they display emergent properties, both structurally and functionally.Such a...

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mentioning

confidence: 99%

Exceptionally stable, redox-active supramolecular protein assemblies with emergent properties

Brodin

Carr

Sontz

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

105

107

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“…The biofunctionalization of NT-WS 2 opens new opportunities for integrating this important group of nanomaterials in composites (e.g., textiles), [22] for the attachment of electronically active components to the sidewalls of the tubes as for example porphyrin ligands for solar cell applications, metal or semiconductor nanoparticles, [9] or for the hierarchical self-assembly of nanoparticles through surface-bound ligands. [23] …”

Section: Discussionmentioning

confidence: 99%

Enzyme‐Mediated Deposition of a TiO₂ Coating onto Biofunctionalized WS2 Chalcogenide Nanotubes

Tahir

Natálio

Therese

et al. 2009

Adv Funct Materials

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A chemically specific and facile method for the biofunctionalization of WS2 nanotubes (NT‐WS2) is reported. The covalent modification strategy is based on the affinity of the nitrilotriacetic acid (NTA) side chain, which serves as a ligand for the surface binding to NT‐WS2 and simultaneously as an anchor group for the binding of His‐tagged proteins to the polymer backbone. The polymer functionalized WS2 nanotubes can be solubilized either in water or organic solvents; they are stable for at least one week. The probes were characterized by FT‐IR and UV‐vis spectroscopy. The immobilization of silicatein, a hydrolytic protein encountered in marine sponges, was visualized by scanning force microscopy (SFM) and confocal laser scanning microscopy (CLSM). The formation of the biotitania coating mediated by the immobilized silicatein onto the surface was characterized by scanning electron microscopy (SEM), and transmission electron microscopy (TEM).

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“…3). [18] Research of the Woggon group is focused on new enzymes, enzymatic reaction mechanisms and the synthesis of inhibitors and catalytically competent enzyme models. In this context the group has discovered two enzymes related to the biosynthesis of vitamins (Scheme 1): i) a non-heme iron protein (60.3 kD) catalyzing the central cleavage of b-carotene 1 to retinal 2 [1,2] and the enzyme tocopherol cyclase (41.3 kD) which cyclizes phytylhydroquinones such as 3 to γ-tocopherol 4, one of the members of vitamin E compounds.…”

Section: Artificial Metalloenzymes: Enantioselective Catalysis and Bementioning

confidence: 99%

Bioorganic and Bioinorganic Chemistry

Constable¹,

Housecroft²,

Creus³

et al. 2010

Chimia

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The interdisciplinary projects in bioinorganic and bioorganic chemistry of the Department of Chemistry, University of Basel led to the preparation of new systems that mimic biologically important processes and to the discovery of compounds from natural sources which are very promising with respect to medical applications. The advances in these areas are reported here. The Constable-Housecroft group is involved in biomimetic and bioinspired chemistry primarily relating to the fundamental processes in nature with a target of designing functional systems of sufficient robustness for practical application. This overview concentrates upon chemical approaches to the splitting of water into H 2 and O 2 based upon biomimetic or bioinspired principles. Other activities within the group involve the tagging of biomolecules with luminescent metal complexes for intracellular studies (with Prof. Matthias Wymann, Department of Biomedicine) and the deployment of nanoparticles functionalised with laccase for water purification (with Prof. Uwe Pieles, FHNW). The group has adopted a general strategy of mimicking the biological membrane by using two-dimensional control of the self-assembly of multiple components at solid-liquid or solid-air interfaces.The ultimate aim for energy production is a coupled water-splitting reaction using solar energy to generate H 2 and O 2 followed by recombination of the two diatomics in a fuel cell. The formal process is presented in Fig. 1 which serves to highlight the features that need to be controlled. Water oxidation and reduction reactions will take place at spatially separated sites and the kinetics of charge separation will be critical in controlling the success of the devices. The device can be purely molecular or the oxidation and reduction equivalents could be holes and electrons in the valence and conduction bands of a semiconductor or a combination of both approaches.For the water reduction process the group has taken inspiration from the Fe 2 S 2 structural motif found in Fe-Fe hydrogenases and invested significant synthetic Biomimetic Splitting of WaterEdwin C. Constable and Catherine E. Housecroft Evolutionary Rules for Metalloenzyme Design Marc CreusCurrent work within the newlyestablished Creus group is based on the concept that direct metal coordination to non-catalytic proteins may lead to nascent metalloenzymes, which can form a basis for further evolution. Conceptually, the dual ideas of 'emergence' and 'evolution' of metalloenzymes correspond to two extremes of an evolutionary process: i) a qualitative step, involving a new function reached through innovation (e.g. acquisition of catalytic activity from previously inactive form) and ii) a quantitative step (e.g. the improvement of a weak, but detectable function). Directed evolution, despite its limitations in practical terms, [1] provides a powerful tool for the second type (or quantitative) step. [2] Although the study of intermediate steps of improvement of metalloenzymes is likely to contribute greatly to the ...

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Hierarchical Self‐Assembly of One‐Dimensional Streptavidin Bundles as a Collagen Mimetic for the Biomineralization of Calcite

Cited by 117 publications

References 82 publications

Exceptionally stable, redox-active supramolecular protein assemblies with emergent properties

Exceptionally stable, redox-active supramolecular protein assemblies with emergent properties

Enzyme‐Mediated Deposition of a TiO₂ Coating onto Biofunctionalized WS2 Chalcogenide Nanotubes

Bioorganic and Bioinorganic Chemistry

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Hierarchical Self‐Assembly of One‐Dimensional Streptavidin Bundles as a Collagen Mimetic for the Biomineralization of Calcite

Cited by 117 publications

References 82 publications

Exceptionally stable, redox-active supramolecular protein assemblies with emergent properties

Exceptionally stable, redox-active supramolecular protein assemblies with emergent properties

Enzyme‐Mediated Deposition of a TiO2 Coating onto Biofunctionalized WS2 Chalcogenide Nanotubes

Bioorganic and Bioinorganic Chemistry

Contact Info

Product

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Enzyme‐Mediated Deposition of a TiO₂ Coating onto Biofunctionalized WS2 Chalcogenide Nanotubes