High affinity YY1 binding motifs: identification of two core types (ACAT and CCAT) and distribution of potential binding sites within the human β globin cluster

Yant, Stephen R.; Zhu, Wenjun; Millinoff, David; Slightom, Jerry L.; Goodman, Morris; Gumucio, Deborah L.

doi:10.1093/nar/23.21.4353

Cited by 140 publications

(104 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, three subunits of the respiratory chain are regulated by this factor, a multifunctional zinc-finger protein, variously referred to as NF-E1 (31), ␦ (38), UCRBP (39), CF-1 (40), F-ACT 1 (41), and NMP-1 (42), that can activate, repress, or initiate transcription. The presence of two or more binding sites for YY1 and their location 3Ј to the transcriptional start site have been frequently observed (32,(42)(43)(44)(45).…”

Section: Discussionmentioning

confidence: 99%

Structural Organization and Promoter Analysis of the Bovine Cytochrome c Oxidase Subunit VIIc Gene

Seelan

Grossman

1997

Journal of Biological Chemistry

View full text Add to dashboard Cite

Cytochrome c oxidase (COX) subunit VIIc is one of the nuclear encoded subunits of the 13-subunit holoenzyme that carries out the terminal step in the electron transport chain. We have isolated the gene for this subunit, previously shown to be ubiquitously expressed from a single copy gene in the genome, and show that 167 base pairs of DNA surrounding the transcriptional start site contain the minimal promoter of this gene. This basal promoter contains two YY1 sites and at least one site for NRF-2, which show binding to their cognate factors. Mutation of both YY1 sites eliminates most of the promoter activity. Mutation at the upstream YY1 site significantly reduces the efficiency of transcript initiation at the major start site and thus plays the dominant role in COX7C regulation. COX7C is, thus, the second nuclear gene of COX that is regulated by YY1, suggesting that it is a third common factor, along with NRF-1 and NRF-2, to be associated with COX gene regulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Structural Organization and Promoter Analysis of the Bovine Cytochrome c Oxidase Subunit VIIc Gene

Seelan

Grossman

1997

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Depending on the DNA sequence context, YY1 can function as an activator (37,38), a repressor (24,39,40), or an initiator of transcription (41,42). The YY1-binding element within the hTOP3 promoter matches well the consensus sequence proposed for the YY1 binding (43)(44)(45). Previously, a number of composite binding elements, in which YY1 competes for occupation by another transcription factor, have been described (24,40,46).…”

Section: Fig 3 Northern Analysis Of Htop3 Mrna Levels In Different mentioning

confidence: 79%

Cloning and Characterization of the 5′-Flanking Region for the Human Topoisomerase III Gene

Kim¹,

Yoon²,

Koo³

et al. 1998

Journal of Biological Chemistry

View full text Add to dashboard Cite

The human DNA topoisomerase III (hTOP3) gene encodes a topoisomerase homologous to the Escherichia coli DNA topoisomerase I subfamily. To understand the mechanisms responsible for regulating hTOP3 expression, we have cloned the 5-flanking region of the gene coding for the hTOP3 and analyzed its promoter activity. The presence of a single transcription initiation site was suggested by primer extension analysis. The hTOP3 gene promoter is moderately high in GC content and lacks a canonical TATA box, suggesting that hTOP3 promoter has overall similarity to promoters of a number of housekeeping genes. Examination of the promoter sequence indicated the presence of four Sp-1 consensus binding sequences and a putative initiator element surrounding the transcription initiation site. Transient expression of a luciferase reporter gene under the control of serially deleted 5-flanking sequences revealed that the 52-base pair region from ؊326 to ؊275 upstream of the transcription initiation site includes a positive cisacting element(s) for the efficient expression of hTOP3 gene. On the basis of gel mobility shift and supershift assays, we demonstrated that both YY1 and USF1 transcription factors can bind to the 52-base pair region. When HeLa cells were transiently transfected with a mutant construct which had disabled both YY1-and USF1-binding sites, the luciferase activity was greatly reduced, suggesting that these binding elements play a functional role in the basal activation of the hTOP3 promoter. Transfection studies with mutations that selectively impaired YY1 or USF1 binding suggested that both YY1 and USF1 function as activators in the hTOP3 promoter.DNA topoisomerases are nuclear enzymes that are able to break and reseal the sugar-phosphate backbone bonds of DNA and thereby adjust the topological states of DNA (1-4). The existence of multiple topoisomerases in both prokaryotes and eukaryotes has been documented. Eukaryotic DNA topoisomerase I catalyzes the removal of both positive and negative supercoils by transiently breaking one strand of the DNA double helix and therefore changing the linking number of DNA in steps of one (5). This mechanism clearly distinguishes the type I from type II enzymes. DNA topoisomerase II catalyzes the strand passing reaction by making transient double-strand breaks and consequently changing the linking number of DNA in steps of two (4, 6). This enzyme catalyzes the ATP-dependent relaxation of negative and positive supercoils, knotting, unknotting, catenation, and decatenation of DNA circles. DNA topoisomerase II is essential for cell viability, and a number of functions have been ascribed to this protein (7-9).In Saccharomyces cerevisiae, the third subfamily of DNA topoisomerases was originally identified from a hyper-recombination mutant that causes an increase in recombination between repetitive DNA elements (10). Null mutation of the topoisomerase III (TOP3) 1 gene displays its pleiotropic phenotypes including slow growth, hyper-recombination, and a defect in sporulation. cDNA seq...

show abstract

“…There exists a potential initiation site ͗ACAT͘ at −27 which is similar to a YY1 core binding sequence and could be the place for binding of the RNA-polymerase II complex to direct the expression of IL-10 in EBV-positive B cells. 45,46 Another explanation for the reduced IL-10 promoter activity in the TACAAAATAA mutant could be the destruction of the imperfect NF-Y/CCAAT-site at this place.…”

Section: Discussionmentioning

confidence: 99%

“…47,48 A potential initiation site at −27 which is similar to a YY1 core binding sequence could be a reason for this inhibition. 45,46 In the area between −681 and −611 there are so far no known sequences for transcription factors identified which could act as a silencer.…”

Section: Discussionmentioning

confidence: 99%

The AT-rich region between −54 to −66 is important for the promoter activity of interleukin-10 in Epstein–Barr virus positive Burkitt’s lymphoma cells

et al. 1999

View full text Add to dashboard Cite

IL-10 is an important regulatory cytokine. The recent characterization of the 5Ј-flanking region of IL-10 led to the identification of the promoter region. The infection of B cells with EBV induces IL-10 production which may contribute to EBV-induced transformation. In the present report, IL-10 promoter elements involved in the constitutive expression of IL-10 in EBV-positive lymphoma cells are described. The AT-rich region between −54/−66 from the transcriptional start site was found to be important for IL-10-promoter activity in BL36. A point mutation at position −60 (T/A) was associated with over 90% reduction of luciferase activity in the cell lines BL36 and BL74. The conversion of A/T at position −57 led to enhanced promoter activity. In addition the AT-rich region could serve as an enhancer for the ␤-globin basic promoter. In BJAB cells (EBV-negative), sequences between −205/−139 rather than the AT-rich region were involved in IL-10 promoter regulation. This underlines the importance of the AT-rich region for EBV-associated IL-10 promoter regulation. Our results further the understanding of how the IL-10 gene could be regulated in B cell lymphomas.

show abstract

High affinity YY1 binding motifs: identification of two core types (ACAT and CCAT) and distribution of potential binding sites within the human β globin cluster

Cited by 140 publications

References 35 publications

Structural Organization and Promoter Analysis of the Bovine Cytochrome c Oxidase Subunit VIIc Gene

Structural Organization and Promoter Analysis of the Bovine Cytochrome c Oxidase Subunit VIIc Gene

Cloning and Characterization of the 5′-Flanking Region for the Human Topoisomerase III Gene

The AT-rich region between −54 to −66 is important for the promoter activity of interleukin-10 in Epstein–Barr virus positive Burkitt’s lymphoma cells

Contact Info

Product

Resources

About