High Biliary Excretion Levels of Quercetin Metabolites after Administration of a Quercetin Glycoside in Conscious Bile Duct Cannulated Rats

Matsukawa, Noriko; Matsumoto, Megumi; Hara, Hiroshi

doi:10.1271/bbb.90031

Cited by 19 publications

(10 citation statements)

References 13 publications

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“…The increased accumulation of quercetin in the livers of rats receiving hydrolyzed extract resulted in the increase of total antioxidant status of the liver and consequently the decreased fatty acids oxidation in the hepatocytes cell membranes. These results are in accordance with many other studies, which indicated the possibilities of the use of biologically active substances of plant origin as a effective strategy for the prevention from the damaging activity of free radicals in intracellular fluids and in cell membranes (Matsukawa et al, 2009;Egert et al, 2008). Also transcriptomic data clearly show that the non-hydrolyzed linden extract regulates the expression of genes involved in the prevention from free radical oxidative damage and the regeneration after oxidative damage.…”

Section: Discussionsupporting

confidence: 91%

The influenc of hydrolyzed and non-hydrolyzed linden inflorescence (<i>Tilia cordata</i>) extract on metabolic and transcriptomic profile in rat liver

Wilczak¹,

Łój²,

Prostek³

et al. 2013

J. Anim. Feed Sci.

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Section: Discussionsupporting

confidence: 91%

The influenc of hydrolyzed and non-hydrolyzed linden inflorescence (<i>Tilia cordata</i>) extract on metabolic and transcriptomic profile in rat liver

Wilczak¹,

Łój²,

Prostek³

et al. 2013

J. Anim. Feed Sci.

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“…The low urinary recovery (1.5%) of the ingested quercetin leaves a large amount of the ingested dose unaccounted for compared with hesperetin and naringenin. The most likely fate of the absorbed quercetin is excretion via bile (Matsukawa et al, 2009) and further degradation to low-molecular-weight phenolic acids that were not analysed in the current and most previous flavonol bioavailability studies (Mullen et al, 2006(Mullen et al, , 2008. Only small amounts of the 3 0 -methylated form of quercetin, isorhamnetin, were present in the 'juice mix' (1.8 mg/500 ml 'juice mix'), and of this only 4.1 ± 3.3% of isorhamnetin was excreted in urine after 48 h, excluding extensive methylation of quercetin.…”

Section: Resultsmentioning

confidence: 99%

Relative bioavailability of the flavonoids quercetin, hesperetin and naringenin given simultaneously through diet

et al. 2010

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The bioavailability and urinary excretion of three dietary flavonoids, quercetin, hesperetin and naringenin, were investigated. Ten healthy men were asked to consume a 'juice mix' containing equal amounts of the three flavonoids, and their urine and plasma samples were collected. The resulting mean plasma area under the curve (AUC) 0À48h and C max values for quercetin and hesperetin were similar, whereas the AUC 0À48h of naringenin and, thus, the relative bioavailability were higher after consumption of the same dose. The study consolidates a significantly lower urinary excretion of quercetin (1.5 ± 1%) compared with hesperetin (14.2±9.1%) and naringenin (22.6±11.5%) and shows that this is not due to a lower bioavailability of quercetin, but rather reflects different clearance mechanisms. ( To compare the impact of dietary important flavonoids, it is necessary to study them in the same food matrix and at similar realistic doses. The present study investigates the bioavailability and urinary excretion of the flavonoids, quercetin, hesperetin and naringenin, in a 48-h intervention study with a single dose (6.3 ml/kg b.w.) of 'juice mix' containing the three flavonoids. European Journal of Clinical NutritionComplete urine and plasma samples were obtained from 10 healthy men, aged 21-28 years. The study was approved by the ethics committee of Copenhagen and Frederiksberg municipality (J.No.(KF)01-161/01). The 'juice mix' was provided to fasting individuals in the morning, along with a standardized flavonoid-free diet (0-24 h), after which the individuals maintained a flavonoid-free diet (24-48 h). Blood and urine samples were collected as described previously (Nielsen et al., 2006). Flavonoid aglycones were quantified in the 'juice mix' (30 mg/l quercetin, 28 mg/l naringenin and 32 mg/l hesperetin) and flavonoid glycosides in the 'juice mix' were identified according to Breinholt et al. (2003).Flavonoids in plasma were completely hydrolysed as described in Nielsen et al. (2006). Plasma samples were added 25 ml aqueous ascorbic acid (20 mg/ml) and 0.5% formic acid to pH ¼ 4 and applied to Evolute ABN columns (25 mg, Mikrolab, Aarhus, Denmark). The eluted flavonoid aglycones were evaporated to dryness and re-dissolved in 200 ml 0.5% formic acid and 10% methanol, and 250 ng 13 C-daidzein was added as external standard.Determination of flavonoids in urine is essentially described elsewhere (Nielsen et al., 2006), except for the inclusion of solid-phase extraction (Isolute SPE 100, Mikrolab) before injection into the liquid chromatographymass spectrometry system. Statistical analyses were performed using Wilcoxon matched pair tests (SPSS version 14.0). The relative

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“…However, the metabolites through enterohepatic circulation and unabsorbed flavonoids reach the large intestine (38). We estimated the FIGURE 7 FD-4 permeability in the colons of mice administered water or DSS in combination with feeding diets without or with naringenin (Expt.…”

Section: Discussionmentioning

confidence: 99%

Supplemental Naringenin Prevents Intestinal Barrier Defects and Inflammation in Colitic Mice

Azuma

Shigeshiro

Kodama³

et al. 2013

The Journal of Nutrition

134

108

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Intestinal barrier defects are involved in the pathogenesis of inflammatory bowel disease. The present study investigated the ameliorative effects of naringenin, a citrus polyphenol, on intestinal tight junction (TJ) barrier defects and inflammation in a murine model of colitis. In Expt. 1, using a 2 × 2 fractional design, the mice were administered water or 2% dextran sulfate sodium (DSS) in combination with feeding control or naringenin-containing diets for 9 d (severe disease stage). DSS administration caused severe colon damage and inflammation, as indicated by body weight loss, increased clinical sores, colon shortening, and gene expressions of inflammatory cytokines [interferon-γ, interleukin (IL)-6, macrophage inflammatory protein-2, and IL-17A). DSS administration also impaired TJ barrier integrity in the colon, as indicated by increased colon permeability and plasma LPS-binding protein levels, resulting from the impaired colonic expression of TJ proteins, occludin, junctional adhesion molecule-A, and claudin-3. Supplemental feeding with naringenin totally or partially attenuated these symptoms, suggesting that naringenin ameliorates the DSS-induced colitis at least partially through protection of the TJ barrier. In Expt. 2, analyses were performed at different disease stages (d 3, 6, and 9) to more widely examine the ameliorative role of naringenin on the initiation and development of colitis. DSS administration moderately induced colon shortening at d 3 and 6 and increased the disease activity index (DAI) and inflammatory cytokine (IL-6 and IL-17A) expression without any significant increases in colonic permeability. Feeding naringenin attenuated the increased DAI and colon shortening and tended to suppress the increased cytokine expression. These findings suggest that the presence of an additional mechanism underlying the naringenin-mediated, anticolitic effect along with barrier protection.

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High Biliary Excretion Levels of Quercetin Metabolites after Administration of a Quercetin Glycoside in Conscious Bile Duct Cannulated Rats

Cited by 19 publications

References 13 publications

The influenc of hydrolyzed and non-hydrolyzed linden inflorescence (<i>Tilia cordata</i>) extract on metabolic and transcriptomic profile in rat liver

The influenc of hydrolyzed and non-hydrolyzed linden inflorescence (<i>Tilia cordata</i>) extract on metabolic and transcriptomic profile in rat liver

Relative bioavailability of the flavonoids quercetin, hesperetin and naringenin given simultaneously through diet

Supplemental Naringenin Prevents Intestinal Barrier Defects and Inflammation in Colitic Mice

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