High-cell-density culture enhances the antimicrobial and freshness effects of Bacillus subtilis S1702 on table grapes (Vitis vinifera cv. Kyoho)

Zhang, Bo; Li, Yao; Yang-yan, Zhang; Qiao, Huitian; He, Jiatai; Yuan, Quan; Chen, Xiangning; Fan, Jie

doi:10.1016/j.foodchem.2019.02.050

Cited by 32 publications

(16 citation statements)

References 28 publications

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“…The mass spectrum peaks of them corresponded to the ion addition peaks of C16 Fengycin A, C16 Fengycin C, and C16 Fengycin D ( Table 4 ). The results were similar to those of Youyou Wang [ 36 ] and Bo Zhang [ 37 ]. These results indicated that strain E2 could produce two LPs; Iturin and Fengycin.…”

Section: Resultssupporting

confidence: 89%

Screening of Bacillus velezensis E2 and the Inhibitory Effect of Its Antifungal Substances on Aspergillus flavus

Zhao

et al. 2022

Foods

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Aspergilus flavus is the main pathogenic fungus that causes food mold. Effective control of A. flavus contamination is essential to ensure food safety. The lipopeptides (LPs) produced by Bacillus strains have been shown to have an obvious antifungal effect on molds. In this study, an antagonist strain of Bacillus velezensis with obvious antifungal activity against A. flavus was isolated from the surface of healthy rice. Using HPLC-MS analysis, the main components of LPs produced by strain E2 were identified as fengycin and iturins. Further investigations showed that LPs could inhibit the spore germination, and even cause abnormal expansion of hyphae and cell rupture. Transcriptomic analyses showed that some genes, involved in ribosome biogenesis in eukaryotes (NOG1, KRE33) and aflatoxin biosynthesis (aflK, aflR, veA, omtA) pathways in A. flavus were significantly down-regulated by LPs. In conclusion, this study provides novel insights into the cellular and molecular antifungal mechanisms of LPs against grain A. flavus contamination.

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Section: Resultssupporting

confidence: 89%

Screening of Bacillus velezensis E2 and the Inhibitory Effect of Its Antifungal Substances on Aspergillus flavus

Zhao

et al. 2022

Foods

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“…Our results revealed that the pre-treatment of grape berries with a culture broth containing both Bvel1 bacterial cells and their secreted metabolites resulted in the colonization of wounded grape berries and a remarkable reduction in the grey mold ingress and growth on wounds 3 or 6 days after artificial inoculation. Our data are in agreement with previous observations, where it was demonstrated that the treatment of fruits and vegetables with cell suspensions, cell cultures, CFCS, or CFCS-extracted metabolites from BCAs significantly reduced the ingress and growth of pre-harvest and post-harvest pathogenic fungi [ 50 , 51 , 52 ]. However, recent studies have demonstrated that effective biocontrol is achieved when the concentration of applied/produced pure elicitors such as plipastatin, iturin, and bacillomycin D on the target tissue is in micromolar amounts (10–50 μM) [ 16 , 44 , 47 , 53 ]; a concentration that is almost equal to their MIC values [ 16 , 51 ].…”

Section: Discussionsupporting

confidence: 93%

Genomic Analysis and Secondary Metabolites Production of the Endophytic Bacillus velezensis Bvel1: A Biocontrol Agent against Botrytis cinerea Causing Bunch Rot in Post-Harvest Table Grapes

Nifakos

Tsalgatidou

Thomloudi

et al. 2021

Plants

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Botrytis bunch rot caused by Botrytis cinerea is one of the most economically significant post-harvest diseases of grapes. In the present study, we showed that the bacterial strain Bvel1 is phylogenetically affiliated to Bacillus velezensis species. The strain Bvel1 and its secreted metabolites exerted an antifungal activity, under in vitro conditions, against B. cinerea. UHPLC–HRMS chemical analysis revealed that iturin A2, surfactin-C13 and -C15, oxydifficidin, bacillibactin, L-dihydroanticapsin, and azelaic acid were among the metabolites secreted by Bvel1. Treatment of wounded grape berries with Bacillus sp. Bvel1 cell culture was effective for controlling grey mold ingress and expansion in vivo. The effectiveness of this biological control agent was a function of the cell culture concentration of the antagonist applied, while preventive treatment proved to be more effective compared to curative. The strain Bvel1 exhibited an adequate colonization efficiency in wounded grapes. The whole-genome phylogeny, combined with ANI and dDDH analyses, provided compelling evidence that the strain Bvel1 should be taxonomically classified as Bacillus velezensis. Genome mining approaches showed that the strain Bvel1 harbors 13 antimicrobial biosynthetic gene clusters, including iturin A, fengycin, surfactin, bacilysin, difficidin, bacillaene, and bacillibactin. The results provide new insights into the understanding of the endophytic Bacillus velezensis Bvel1 biocontrol mechanism against post-harvest fungal pathogens, including bunch rot disease in grape berries.

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“…Moreover, recent studies reported that B. subtilis can produce not only antibiotics and antifungal proteins but also a series of volatile organic compounds (VOCs), which can effectively inhibit the growth of postharvest pathogens of fruits and vegetables. In our previous study, the B.…”

Section: Introductionmentioning

confidence: 99%

Antifungal Mechanism of Volatile Organic Compounds Produced by Bacillus subtilis CF-3 on Colletotrichum gloeosporioides Assessed Using Omics Technology

Wang

Qin

et al. 2021

J. Agric. Food Chem.

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Bacillus subtilis is commonly used as a biocontrol bacterium owing to its strong antifungal activity, broad-spectrum inhibition, and general safety. In this study, the inhibitory effects of volatile organic compounds (VOCs) produced by B. subtilis CF-3 on Colletotrichum gloeosporioides, a major destructive phytopathogen of litchi anthracnose, were analyzed using proteomics and transcriptomics. Differentially expressed genes (DEGs) and proteins (DEPs) indicated that the inhibition of C. gloeosporioides by B. subtilis CF-3 VOCs downregulated the expression of genes related to cell membrane fluidity, cell wall integrity, energy metabolism, and production of cell wall-degrading enzymes. Particularly, those important DEGs and DEPs related to the ergosterol biosynthetic and biosynthesis of unsaturated fatty acids are most significantly influenced. 2,4-di-tert-butylphenol, a characteristic component of B. subtilis CF-3 VOCs, also showed a similar effect on C. gloeosporioides. Our results provide a theoretical basis for the potential application of B. subtilis CF-3 in the postharvest protection of fruits and vegetables.

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High-cell-density culture enhances the antimicrobial and freshness effects of Bacillus subtilis S1702 on table grapes (Vitis vinifera cv. Kyoho)

Cited by 32 publications

References 28 publications

Screening of Bacillus velezensis E2 and the Inhibitory Effect of Its Antifungal Substances on Aspergillus flavus

Screening of Bacillus velezensis E2 and the Inhibitory Effect of Its Antifungal Substances on Aspergillus flavus

Genomic Analysis and Secondary Metabolites Production of the Endophytic Bacillus velezensis Bvel1: A Biocontrol Agent against Botrytis cinerea Causing Bunch Rot in Post-Harvest Table Grapes

Antifungal Mechanism of Volatile Organic Compounds Produced by Bacillus subtilis CF-3 on Colletotrichum gloeosporioides Assessed Using Omics Technology

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