High-density lipoprotein subfractions in normolipidemic individuals without clinical atherosclerosis lipoprotein subfractions in an adult population

Abstract

This study evaluated the serum concentrations of lipids, lipoproteins, apolipoproteins, and high-density lipoprotein (HDL) subfractions in Brazilian adults. We analyzed the distribution of lipids in HDL2 and HDL3 in a normolipidemic population without evidence of established cardiovascular disease (CVD). A total of 93 males and 92 females, healthy and normolipidemic, volunteered to be submitted to a clinical examination, a blood collection, and to answer a questionnaire aimed at determining signs and symptoms of atherosclerotic disease. Their fasting plasma lipid, lipoproteins, apolipoproteins, and the cholesterol and triglyceride concentrations in HDL2 and HDL3, isolated by microultracentrifugation, were determined by enzymatic-colorimetric methods. The interpercentile intervals (2.5-97.5) for the population were established as being 5-18 mg/dL in men and 4-28 mg/dL in women for HDL2 cholesterol (HDL2chol) and 1-57 mg/dL in men and 2-61 mg/dL in women for HDL3 cholesterol (HDL3chol). HDL2 triglyceride levels (HDL2Tg) in men were 1-26 mg/dL and in women 2-28 mg/dL; moreover, the HDL3 triglyceride (HDL3Tg) intervals were established as 4-46 mg/dL for both sexes. The determination of reference ranges for lipids in HDL subfractions in populations without clinical atherosclerosis, is an useful tool for metabolic, diagnostic, and therapeutic approaches. We determined the intervals for HDL2chol, HDL3chol, HDL2Tg, and HDL3Tg. There were variations with sex and/or age for HDL2chol, HDL3chol, and HDL2Tg in the studied population.

Reference Ranges of Cholesterol Sub-Fractions in Random Healthy Adults in Ouagadougou, Burkina Faso

“…The values of HDL2-c in our study are similar to Western values [ 20 , 22 , 26 ] and those of Brazil [ 27 ]. Comparison by gender showed a significant increase in HDL2-c in women (p = 0.02).…”

“…The reference values of HDL3-c observed in the study population were lower than those in London [ 26 ] and French values [ 20 , 22 ] but similar to the Brazilian values [ 27 ]. In addition, our HDL3-c values showed no significant difference according to age and sex.…”

“…This agrees with Atger [ 22 ] and Roche [ 20 ] in France. On contrary in Brazil, Sodré [ 27 ] reported a significant increase in HDL 3-c in women.…”

Relative contributions of L-FABP, SCP-2/SCP-x, or both to hepatic biliary phenotype of female mice

“…Studies reporting a higher incidence of non-alcoholic fatty liver disease (NAFLD) and higher hepatic triglyceride accumulation in males than females, an increasing rate of NAFLD in postmenopausal women, and severe hepatic steatosis in mice ablated in aromatase suggest that NAFLD may be estrogen-associated [69–73]. Further, high-density lipoprotein-2 (HDL2) particles from females are larger and more capable of mediating cholesterol efflux from peripheral tissues [74;75]. …”

Metformin Increases HDL3–Cholesterol and Decreases Subcutaneous Truncal Fat in Nondiabetic Patients with HIV-Associated Lipodystrophy

“…HDL2 and HDL3 subfractions were separated from this sobrenadant by microultracentrifugation in Airfuge (Beckman, Fullerton, CA), carried out in Laboratory of Clinical Pathology, State University of Campinas (UNICAMP). 8 Chol and Tg were measured in these subfractions by commercial kits (Roche) in automatized Cobas Mira (Roche). Plasma nascent HDL-chol was calculated by: [plasma total HDL-chol -(HDL2-chol ϩ HDL3-chol)].…”