High density of specific GABA binding sites in the human fallopian tube

Erdö, Sándor L.; László, Ádám; Szporny, L; Zsolnai, B

doi:10.1016/0304-3940(83)90399-3

Cited by 27 publications

(17 citation statements)

References 19 publications

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“…The occurrence of GABA and specific GABA-A-type binding sites in the human ovary [9] and oviduct [8] has previously been demonstrated in this laboratory. In these studies, however, only few (4-5) organs were exam ined, which did not allow one to correlate the biochemi cal data with parameters like the age or hormonal back ground of the patients.…”

Section: Discussionmentioning

confidence: 99%

“…Under such conditions, labelled GABA is known to bind to GABA-A sites selectively [8,9,18]. Attempts to measure the specific binding to GABA-B sites were also made, but the non specific binding was so high that the results could not be evaluated with confidence.…”

Section: Biochemical Assay Proceduresmentioning

confidence: 99%

“…Surgery was performed after conventional premedication, under anesthesia [8,9] because of uterine myoma (n = 21) or carcinoma (n = 4), ovarian cysts (n = 3) or tubal preg nancy (n = 3). Organs with (organoleptically) visible tumorous tissue were excluded from the biochemical examination.…”

Section: Patients and Organsmentioning

confidence: 99%

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Gamma-Aminobutyric Acid, Its Related Enzymes and Receptor-Binding Sites in the Human Ovary and Fallopian Tube

László¹,

Villányi²,

Zsolnai³

et al. 1989

Gynecol Obstet Invest

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The concentration of Γ-aminobutyric acid (GABA), the activities of related enzymes, i.e. glutamate decarboxylase and GABA transaminase, as well as the level of specific GABA binding sites were determined in ovaries and fallopian tubes obtained surgically from 31 women. None of the biochemical parameters examined showed a correlation with the age and hormonal background (serum estradiol and progesterone levels) of the patients. The respective ovarian and tubal values did not differ significantly in groups operated on because of uterine myoma and carcinoma. In organs from pregnant women, however, most GABAergic markers altered significantly. These findings indicate some gestation-related role for the ovarian and tubal GABA systems in humans.

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Section: Discussionmentioning

confidence: 99%

Section: Biochemical Assay Proceduresmentioning

confidence: 99%

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Gamma-Aminobutyric Acid, Its Related Enzymes and Receptor-Binding Sites in the Human Ovary and Fallopian Tube

László¹,

Villányi²,

Zsolnai³

et al. 1989

Gynecol Obstet Invest

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“…Ligand binding studies with homogenates revealed the presence of specific, bicuculline-sensitive (GABAA) receptor sites in several organs, such as female sex organs (Erdo & Lapis, 1982ab;Erdo et al, 1983;Erdo, 1984;Erdo & Laszlo, 1984), placenta (Erdo et al, 1985), several types of blood vessels I Author for correspondence at Department of Anatomy, Georg August University, 36 Kreuzbergring, D-3400 Gottingen, F.R.G. (Krause et al, 1980a,b;Napoleone et al, 1987), hepatocytes (Minuk et al, 1987), and adrenal medulla (Kataoka et al, 1984, Martinez et al, 1987.…”

Section: Introductionmentioning

confidence: 99%

Binding of [³H]‐muscimol to GABA_A sites in the guinea‐pig urinary bladder: biochemical assay and autoradiography

Erdö

Mione

Amenta

et al. 1989

British J Pharmacology

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-muscimol in the bladder. The density of binding sites was high in clusters within the smooth muscle layers of the bladder fundus and of the urethra, while the apex and the neck were not specifically labelled. 6 The present findings show that GABAA type receptor sites in the guinea-pig urinary bladder may be labelled by [3H]-muscimol in a specific and reproducible manner. Moreover, the localization of these binding sites is consistent with the presence of GABAA receptors in only a subpopulation of vesical ganglia.

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“…They are also present in peripheral tissues outside of the brain, including the female reproductive tract, where the subunit composition of the GABA A receptor fluctuates in the rat uteri throughout pregnancy. (2,(12)(13)(14)30) Isoform selection affects the sensitivity and responsiveness of the GABA A receptor to progesterone metabolites, which may help regulate parturition. (17).…”

mentioning

confidence: 99%

HOXA10 regulates endometrial GABA_Aπ receptor expression and membrane translocation

Sadeghi

Taylor

2010

American Journal of Physiology-Endocrinology and Metabolism

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of the GABA A receptor has been described previously in the human endometrium in both luminal epithelium and stroma. Its expression is increased during decidualization in rodents and in the implantation window of human endometrium. Here we localized GABA subunit receptor protein in human endometrium and identified regulators of gene expression and activation. GABA A was localized to the cell surface, and expression increased during the window of embryo implantation in human endometrium. The well-differentiated human endometrial adenocarcinoma cell line Ishikawa was treated with progesterone and transfected with pcDNA-HOXA10, HOXA10 siRNA, or respective controls. GABAA receptor mRNA expression was evaluated by real-time RT-PCR. Protein expression and localization were evaluated using immunofluorescence. GABAA receptor mRNA expression was increased significantly after either progesterone treatment or HOXA10 transfection. Coadministration of progesterone along with HOXA10 transfection had no additional effect on the expression of GABAA receptor mRNA over either agent alone. Blocking HOXA10 expression with siRNA prevented progesteroneinduced GABAA receptor mRNA expression. Additionally, either HOXA10 or progesterone independently caused increased translocation of the GABA receptor from the cytoplasm to the cell membrane. Translocation in response to progesterone was blocked with HOXA10 siRNA. Progesterone-induced GABAA subunit receptor expression is likely mediated indirectly through progesterone's regulation of HOXA10 expression. Modification of subtype composition and translocation of the GABA receptor ion channel likely modulate endometrial receptivity. Whereas HOXA10 typically enhances the expression of progesterone-responsive genes, here HOXA10 expression leads to production of a less progestin-responsive GABA receptor subtype, likely buffering the effects of luteal phase progesterone on GABA receptor activity. homeobox A10; ␥-aminobutyric acid

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High density of specific GABA binding sites in the human fallopian tube

Cited by 27 publications

References 19 publications

Gamma-Aminobutyric Acid, Its Related Enzymes and Receptor-Binding Sites in the Human Ovary and Fallopian Tube

Gamma-Aminobutyric Acid, Its Related Enzymes and Receptor-Binding Sites in the Human Ovary and Fallopian Tube

Binding of [³H]‐muscimol to GABA_A sites in the guinea‐pig urinary bladder: biochemical assay and autoradiography

HOXA10 regulates endometrial GABA_Aπ receptor expression and membrane translocation

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High density of specific GABA binding sites in the human fallopian tube

Cited by 27 publications

References 19 publications

Gamma-Aminobutyric Acid, Its Related Enzymes and Receptor-Binding Sites in the Human Ovary and Fallopian Tube

Gamma-Aminobutyric Acid, Its Related Enzymes and Receptor-Binding Sites in the Human Ovary and Fallopian Tube

Binding of [3H]‐muscimol to GABAA sites in the guinea‐pig urinary bladder: biochemical assay and autoradiography

HOXA10 regulates endometrial GABAAπ receptor expression and membrane translocation

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Binding of [³H]‐muscimol to GABA_A sites in the guinea‐pig urinary bladder: biochemical assay and autoradiography

HOXA10 regulates endometrial GABA_Aπ receptor expression and membrane translocation