High diagnostic performance of ELISA detection of antibodies to citrullinated antigens in rheumatoid arthritis

Suzuki, Kazuo; Sawada, Tetsuji; Murakami, Akihiro; Matsui, Toshihiro; Tohma, Shigeto; Nakazono, Kiyoshi; Takemura, Masao; Takasaki, Yoshinari; Mimori, Tsuneyo; Yamamoto, Kazuhiko

doi:10.1080/03009740310003677

Cited by 147 publications

(95 citation statements)

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“…8,14,15 An assay of CCP antibody of second generation was later established and the sensitivity and specificity of the assay were 66%-88% and 89%-98%, respectively. [16][17][18][19][20] The sensitivity and specificity of the anti-citrullinated type II collagen antibody assay were approximately 79% and 95%, respectively. Thus, the anti-citrullinated type II collagen antibody assay may become one of the significant tools for diagnosis of RA, although the sensitivity of the assay is less than that of the CCP antibody assay of second generation.…”

Section: Discussionmentioning

confidence: 99%

Autoimmunity to citrullinated type II collagen in rheumatoid arthritis

et al. 2006

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The production of autoantibodies to citrullinated type II collagen and the citrullination of type II collagen were analyzed in rheumatoid arthritis. Autoantibodies to citrullinated type II collagen were detected in 78.5% of serum samples from 130 rheumatoid arthritis patients. Autoantibodies to native noncitrullinated type II collagen were detected in 14.6% of serum samples, all of which were positive for anti-citrullinated type II collagen antibodies. Serum samples were also positive for anti-citrullinated type II collagen antibodies in 1 of 31 systemic lupus erythematosus patients and 2 of 55 patients with osteoarthritis of the knee. In contrast, sera samples from 24 systemic sclerosis patients, 21 dermatomyositis/polymyositis patients, 21 ankylosing spondylitis patients, and 18 psoriatic arthritis patients were all negative for anti-citrullinated type II collagen antibodies. Anti-citrullinated type II collagen antibodies and fragments of citrullinated type II collagen were found in the synovial fluid obtained from affected knee joints of 15 rheumatoid arthritis patients. Moreover, anticitrullinated type II collagen antibodies were isolated from the synovium of affected knee joints in 8 rheumatoid arthritis patients using antigen/antibody immunocomplex dissociation buffer but not by using standard buffers. These findings indicate that autoantibodies that react with citrullinated type II collagen are specifically produced and that immunocomplexes composed of fragments of citrullinated type II collagen and autoantibodies are deposited in the inflamed articular synovium in rheumatoid arthritis patients. Assaying for the presence of anticitrullinated type II collagen antibodies may therefore be useful for diagnosing rheumatoid arthritis, and the deposition of these immunocomplexes in the articular synovium may be involved in pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Autoimmunity to citrullinated type II collagen in rheumatoid arthritis

et al. 2006

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“…To date, a number of reports have demonstrated the clinical significance of autoantibodies to citrullinated filaggrin and CCP in the diagnosis of RA as summarized in Table 1 (3,5,6,(11)(12)(13)(14). Although the specificity of antifilaggrin/CCP antibodies in RA is more than 90% in almost all reports, the prevalence (sensitivity) of the same antibodies ranges from 33% to 87.2%.…”

Section: Clinical Significance Of Anti-citrullinated Protein Antibodimentioning

confidence: 99%

Clinical Significance of Anti-CCP Antibodies in Rheumatoid Arthritis

Mimori

2005

Intern. Med.

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“…It has relatively low specifi city due to being sometimes detected in patients with other autoimmune diseases and unaffected healthy people. In contrast, anti-CCP antibodies, which have higher specifi city than RF and comparable sensitivity with that of RF (De Rycke et al 2004), are good serologic markers for RA (Suzuki et al 2003;De Rycke et al 2004). Our study confi rms that anti-CCP antibody is more predictive than RF in RA patients.…”

Section: Discussionmentioning

confidence: 32%

“…Anti-CCP antibodies have been proved to be an effi cient diagnostic marker for RA, as they can be detected with high disease specifi city in more than 80% of serum samples taken from RA patients (Suzuki et al 2003). Moreover, anti-CCP antibodies are now considered as important markers for the diagnosis of RA and as probable prognostic markers for the development of erosive arthritis (van der Helm-van Mil et al 2005;Syversen et al 2007).…”

Section: Participants and Methodsmentioning

confidence: 99%