High Dietary Phosphate Intake Reduces Bone Strength in the Growing Rat Skeleton

Huttunen, Minna; Tillman, Ilse; Viljakainen, Heli; Tuukkanen, Juha; Peng, Zhiqi; Pekkinen, Minna; Lamberg‐Allardt, Christel

doi:10.1359/jbmr.061009

Cited by 64 publications

(49 citation statements)

References 40 publications

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“…It has been suggested that a high-P diet contributes to bone loss in humans as well as animals (3)(4)(5). In the present study, a high-P diet decreased serum Ca concentrations and increased serum P concentrations, which caused an increase in serum PTH concentration.…”

Section: Discussionsupporting

confidence: 53%

“…In the present study, food intake and final body weight were lower in rats fed the high-P diet compared with those fed the control diet. Previous studies have also shown that a high-P diet decreases food intake (4,5). Therefore, it is thought that the decreased food intake and body weight associated with a high-P diet influence bone loss.…”

Section: Discussionmentioning

confidence: 99%

“…It increases parathyroid hormone (PTH) secretion (1,2), which causes an increase in bone resorption and a decrease in bone mineral density (BMD) (3)(4)(5). Our previous study demonstrated that elevated PTH secretion from a high-P diet increases mRNA expression of the receptor activator of NF-kB ligand (RANKL), a mediator of osteoclastic bone resorption, resulting in increased osteoclastic bone resorption (4).…”

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confidence: 99%

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Effects of Dietary Calcium Supplementation on Bone Metabolism, Kidney Mineral Concentrations, and Kidney Function in Rats Fed a High-Phosphorus Diet

Katsumata

Matsuzaki

Uehara

et al. 2015

J Nutr Sci Vitaminol

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A high-phosphorus (P) diet has several effects on calcium (Ca) and P metabolism. It increases parathyroid hormone (PTH) secretion (1, 2), which causes an increase in bone resorption and a decrease in bone mineral density (BMD) (3-5). Our previous study demonstrated that elevated PTH secretion from a high-P diet increases mRNA expression of the receptor activator of NF-kB ligand (RANKL), a mediator of osteoclastic bone resorption, resulting in increased osteoclastic bone resorption (4). Moreover, it is well known that a high dietary P concentration is an important etiologic factor in the development of nephrocalcinosis and diminished kidney function. A high-P diet is reportedly associated with increased kidney Ca and P concentrations and nephrocalcinosis development (6-8). and Van Camp et al. (9) reported that urinary albumin excretion, which is positively correlated with kidney Ca concentrations, is elevated in rats fed a high-P diet. Our previous studies also showed that renal tubular function is impaired in rats fed a high-P diet (10,11).Ca supplementation has beneficial effects on bone health, including enhanced bone mineral content (BMC) and BMD (12,13). Epidemiological studies have shown that a lower Ca/P intake ratio is associated with a decrease in BMD (14,15). Our previous study also showed that the dietary Ca/P ratio regulates bone mineralization and turnover in vitamin D receptor knockout mice (16). Therefore, dietary Ca supplementation might prevent bone loss induced by a high-P diet. In addition, we speculated that dietary Ca supplementation may have preventive effects on kidney damage, such as nephrocalcinosis, and impaired kidney function as well as bone loss due to a high-P diet. Therefore, we aimed to clarify the relationships between dietary Ca supplementation and bone loss as well as kidney damage with a high-P diet. The purpose of this study was to investigate the effects of dietary Ca supplementation on bone metabolism, kidney mineral concentrations, and kidney function in rats fed a high-P diet. Materials and MethodsExperimental design. This study was approved by the Tokyo University of Agriculture Animal Use Committee and the rats were maintained in accordance with the university guidelines for the care and use of laboratory animals. Four-week-old male Wistar strain rats were purchased from Clea Japan (Tokyo, Japan) and housed individually in stainless steel cages in a room maintained at 22˚C with a 12-h light-dark cycle. All rats were fed a control diet containing 0.3% P and 0.5% Ca for 7 d of acclimatization. After the acclimatization period, all rats were randomly divided into 4 dietary groups of 6 Summary We investigated the effects of dietary calcium (Ca) supplementation on bone metabolism, kidney mineral concentrations, and kidney function in rats fed a high-phosphorus (P) diet. Wistar strain rats were randomly divided into 4 dietary groups and fed their respective diets for 21 d: a diet containing 0.3% P and 0.5% Ca (C), a diet containing 1.5% P and 0.5% Ca (HP), a diet containing ...

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of Dietary Calcium Supplementation on Bone Metabolism, Kidney Mineral Concentrations, and Kidney Function in Rats Fed a High-Phosphorus Diet

Katsumata

Matsuzaki

Uehara

et al. 2015

J Nutr Sci Vitaminol

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“…In addition, the respective calcium contents of femurs, mandibles, and lumbar vertebrae were significantly decreased . High phosphate (1.2% and 1.8%) intake limits bone growth and increases the number of osteoclasts, and also reduces bone stiffness and toughness in the femoral neck and tibial shaft (Huttunen et al, 2007).…”

Section: Influence Of Pi On Bone Resorption In Animalsmentioning

confidence: 99%

Biological effects of inorganic phosphate: potential signal of toxicity

et al. 2015

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-Inorganic phosphate (Pi) plays crucial roles in several biological processes and signaling pathways. Pi uptake is regulated by sodium-dependent phosphate (Na/Pi) transporters (NPTs). Moreover, Pi is used as a food additive in food items such as sausages, crackers, dairy products, and beverages. However, the high serum concentration of phosphate (> 5.5 mg/dL) can cause adverse renal effects, cardiovascular effects including vascular or valvular calcification, and stimulate bone resorption. In addition, Pi can also alter vital cellular signaling, related to cell growth and cap-dependent protein translation. Moreover, intake of dietary Pi, whether high (1.0%) or low (0.1%), affects organs in developing mice, and is related to tumorigenesis in mice. The recommended dietary allowance (RDA) of Pi is the daily dietary intake required to maintain levels above the lower limit of the range of normal serum Pi concentration (2.7 mg/dL) for most individuals (97-98%). Thus, adequate intake of Pi (RDA; 700 mg/day) and maintenance of normal Pi concentration (2.7-4.5 mg/dL) are important for health and prevention of diseases caused by inadequate Pi intake.

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“…1 Recent epidemiologic and experimental studies have further amplified the role this condition plays in the larger story of CKD-MBD. Experimental studies have demonstrated that high phosphorus plays a key role in the development of vascular calcification 2 and impairment of bone mass and strength, induces changes in the expression pattern of muscle and bone-related genes, 3,4 and may also act as a pro-aging factor. 5 In addition, clinical studies have demonstrated an association among hyperphosphatemia, vascular stiffness, and left ventricular hypertrophy.…”

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confidence: 99%