High dilutions of antimony modulate cytokines production and macrophage – Leishmania (L.) amazonensis interaction in vitro

Santana, Fabiana; Dalboni, Luciane Costa; Nascimento, Katia Fialho do; Konno, Fabiana Toshie de Camargo; Alvares-Saraiva, Anuska Marcelino; Correia, Michelle S; Bomfim, Maristela Dutra Correa; Casarin, Renato Corrêa Viana; Pérez, Elizabeth; Lallo, Maria Anete; Peres, Giovani Bravin; Laurenti, Márcia Dalastra; Benites, N. R.; Buchi, Dorly de Freitas; Bonamin, Leoni Villano

doi:10.1016/j.cyto.2017.01.004

Cited by 21 publications

(28 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…29 It was later identified that the clinical improvement was due to the reduction in certain cytokines, which had increased in response to the infection, and the blocking of chemokine CCL2 (MCP-1), known for monocyte recruitment and parasite multiplication. 30 Similarly, in the present investigation, the decrease in infection may be due to an improvement in the suppressed immune system caused by VL. Similar results were reported by Cajueiro et al, 31 in which BioLi 30x treatments significantly induced morphological alterations and increased nitric oxide production in the in-vitro assay of L. infantum promastigotes.…”

Section: Parasite Loadsupporting

confidence: 70%

The Effect of Iodium 30c on Experimental Visceral Leishmaniasis

et al. 2020

View full text Add to dashboard Cite

Background Leishmaniasis is one of several neglected tropical diseases that warrant serious attention. A disease of socio-economically poor people, it demands safer and cheaper drugs that help to overcome the limitations faced by the existing anti-leishmanials. Complementary or traditional medicines might be a good option, with an added advantage that resistance may not develop against these drugs. Thus, the present investigation was performed to evaluate the anti-leishmanial efficacy of an ultra-diluted homeopathic medicine (Iodium 30c) in experimental visceral leishmaniasis (VL). Methods Compliant with strict ethical standards in animal experimentation, the study was performed in-vivo in inbred BALB/c mice which were injected intravenously with 1 × 107 promastigotes of Leishmania donovani before (therapeutic) or after (prophylactic) treatment with Iodium 30c for 30 days. In other groups of mice (n = 6 per group), amphotericin B served as positive control, infected animals as the disease control, while the naïve controls included normal animals; animals receiving only Iodium 30c or Alcohol 30c served as sham controls. The anti-leishmanial efficacy was assessed by determining the hepatic parasite load and analysing percentages of CD4+ and CD8+ T cells. Biochemical analysis and histological studies were performed to check any toxicities. Results Iodium-treated animals showed a significantly reduced parasite load (to 1503 ± 39 Leishman Donovan Units, LDU) as compared with the infected controls (4489 ± 256 LDU) (p < 0.05): thus, the mean therapeutic efficacy of Iodium 30c was 66.5%. In addition, the population of CD4+ and CD8+ T cells was significantly increased (p < 0.05) after treatment. No toxicity was observed, as evidenced from biochemical and histopathological studies of the liver and kidneys. Efficacy of Iodium 30c prophylaxis was 58.3%, while the therapeutic efficacy of amphotericin B was 85.9%. Conclusion This original study has shown that Iodium 30c had significant impact in controlling parasite replication in experimental VL, though the effect was less than that using standard pharmaceutical treatment.

show abstract

Section: Parasite Loadsupporting

confidence: 70%

The Effect of Iodium 30c on Experimental Visceral Leishmaniasis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…12 The in-vitro effect of homeopathy on living cells is scientifically consolidated by several renowned studies and without requiring animal models. 13 These effects have already been reported for bacteria, 12,14 protozoa, 15,16 fungi, 17 cell cultures, 18,19 and reproductive cells of mammals. 20 Recently, effects of homeopathic medicines have been demonstrated on the bacterium Streptococcus pyogenes.…”

Section: Introductionmentioning

confidence: 78%

“…14 There is also a recent report of intense immunomodulatory effects upon exposure to homeopathic medicines on inflammatory mechanisms of cell cultures infected by the protozoon Leishmania amazonensis due to exposure to homeopathic medicines. 16 Nevertheless, the effect of homeopathy on dipterous larvae has not been scientifically reported to date.…”

Section: Introductionmentioning

confidence: 99%

Effect of Homeopathic Medicines and a Nosode on Larvae of Cochliomyia hominivorax (Diptera: Calliphoridae)

2019

View full text Add to dashboard Cite

Background Cochliomyia hominivorax is the major fly causing primary myiasis in livestock animals in Brazil; its larvae develop in the host's living tissues, causing mutilations, which can even lead to death. In conventional treatments of myiasis, chemo-synthetic insecticides have been employed directly on larvae present in the wounds. Homeopathy may represent a healthy and sustainable alternative both to prevent and to treat myiasis in animals and humans. The current study evaluated how the emergence of adult insects is affected by the use of the homeopathic medicines Sulfur 12cH and Pyrogenium 12cH, and the nosode produced from C. hominivorax larvae at potencies 8cH and 12cH, on third-stage larvae of a C. hominivorax colony. Materials and Methods The homeopathic medicines and the nosodes were produced according to the Brazilian Homeopathic Pharmacopoeia. Control groups were distilled water, alcohol, no substance, and the organophosphate insecticide trichlorfon. For each group, 10 replicates were performed. Emergence rate of adult insects was evaluated by descriptive statistics followed by analysis of variance. Homogeneity of variances was verified by F-test and group means were compared with Tukey's test. Results Mortality rates in control groups were 2.7% for 30% (v/v) alcohol, 4.3% for distilled water, 3.2% for no substance (p > 0.05). In the trichlorfon group, the mortality rate of larvae was 90.8%. For Sulfur 12cH, the mortality of larvae was 94.6%, and for Pyrogenium 12cH it was 98.6%. The latter three means were not statistically different from each other or from the mean found for the trichlorfon group. The mortality rates of larvae were 61.3% and 66.6% for nosode C. hominivorax 8cH and 12cH, respectively (p > 0.05). Conclusions Results suggest that homeopathy could be used therapeutically to prevent and treat animals and humans with myiasis caused by C. hominivorax.

show abstract

“…The mechanism by which ultra-high dilutions interact with solvatochromic dyes is still being elucidated, though evidence so far indicates that such dilutions are polarizing, inducing dipole moment changes. 18 Recently, correspondence between the effects of the homeopathic medicine Antimonium crudum on solvatochromic dyes and the effects of the same dilutions in an in vitro model using macrophages infected with Leishmania amazonensis 22 has been demonstrated. 20 This has opened up new avenues of study in the field of high dilution research and possible mechanisms of action, in which the effects of ultra-high dilutions on solvatochromic dyes could be considered as a physico-chemical indicator of their biological activity.…”

Section: Discussionmentioning

confidence: 99%

“…A previous study developed in our laboratory on the physicochemical properties of the drug Antimonium crudum 19,20 has shown that absorbance variations of solvatochromic dyes presented a clear relation with the biological effects of this drug in vitro and in vivo. 21,22 These findings suggest that solvatochromic dyes could be used as physico-chemical probes of biologically active high dilutions of different substances beyond Avogadro's number, based on dipole moment changes. 23 Discovering if solvatochromic dyes are sensitive enough to detect changes in the polarity of large water volumes would be a critical step in evaluating the plausibility of using high dilutions in many field situations, such as environmental management, 24 organic agriculture 25 and in herd, broiler, and fish breeding, with a view to improving productivity.…”

Section: Introductionmentioning

confidence: 95%

Interaction between Solvatochromic Dyes and Water Sampled from a Natural Source Treated with High Dilutions of Phosphorus

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background Highly diluted and succussed solutions interact with solvatochromic dyes, indicating that changes in solvent and solute polarity could be related to their mechanism of action. It is not known, however, how the activity associated with succussed high dilutions is transferred to untreated water and what the limits of this process are. Aims The aims of the present study were to ascertain whether a succussed high dilution of phosphorus (1.5 × 1−59 M; Phos 30cH) seeded into a natural water source that fed a fjord and two connected lakes could propagate itself through the lake system (total volume 2200 m3) and, moreover, whether the process could be tracked using solvatochromic dyes. Methods Samples of water were collected before and after seeding, at different times and places throughout the lake system. Controls comprised water taken from an untreated and adjacent, but independent, lake (1385 m3). Results Water samples taken up to 72 hours after the source treatment produced significant increases (p ≤ 0.03) in the absorbance of the solvatochromic dye methylene violet (MV), while samples from the control lake produced no changes. Conclusions The study indicates that activity associated with Phos 30c can propagate itself through large volumes of water, causing changes throughout a whole connected lake system, and that these changes can be tracked using the solvatochromic dye MV. This in turn means the use of homeopathic medicines in large volumes of drinking water, in farming and ecological contexts, now has the potential to be assessed with physico-chemical monitoring.

show abstract

High dilutions of antimony modulate cytokines production and macrophage – Leishmania (L.) amazonensis interaction in vitro

Cited by 21 publications

References 62 publications

The Effect of Iodium 30c on Experimental Visceral Leishmaniasis

The Effect of Iodium 30c on Experimental Visceral Leishmaniasis

Effect of Homeopathic Medicines and a Nosode on Larvae of Cochliomyia hominivorax (Diptera: Calliphoridae)

Interaction between Solvatochromic Dyes and Water Sampled from a Natural Source Treated with High Dilutions of Phosphorus

Contact Info

Product

Resources

About