High diversity of invasive Haemophilus influenzae isolates in France and the emergence of resistance to third generation cephalosporins by alteration of ftsI gene

Deghmane, Ala‐Eddine; Hong, Eva; Chehboub, Sara; Terrade, Aude; Falguières, Michaël; Sort, Morgan; Harrison, Odile B.; Jolley, Keith A.; Taha, Muhamed‐Kheir

doi:10.1016/j.jinf.2019.05.007

Cited by 44 publications

(48 citation statements)

References 26 publications

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“…Isolates were cultured onto polyvitex chocolate agar plates and incubated at 37 C in 5% CO 2 for 18-24 h. DNA extraction for polymerase chain reaction (PCR) and next-generation sequencing were performed as previously described (Deghmane et al, 2019).…”

Section: Bacterial Growth Dna Preparationmentioning

confidence: 99%

“…Haemophilus species were initially identified by colony morphology, Gram stain and requirement for growth factors (V-, X-and XV-factors). PCR of the ompP2, bexA, fucK, iga, and hpd genes was applied as previously described (Deghmane et al, 2019). Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) Biotyper, version 3.0 (Bruker Daltonics, Champs sur Marne, France) was performed as previously described (Hong et al, 2019).…”

Section: Bacterial Identificationmentioning

confidence: 99%

“…For chloramphenicol resistance, chloramphenicol acetyltransferase (cat) gene production was searched. For β-lactams, three approaches were considered: production of β-lactamases, mutations in the ftsI gene encoding penicillin-binding protein 3 (PBP3) or both mechanisms (Dabernat et al, 2002;Deghmane et al, 2019). ROB-1 or TEM-1 β-lactamases were determined by DNA sequence comparisons (Livrelli et al, 1991).…”

Section: Molecular Mechanism Of Antibiotic Resistancementioning

confidence: 99%

“…Mutations in the ftsI gene encoding PBP3 were determined using http://pubmlst. org/hinfluenzae/ (Deghmane et al, 2019).…”

Section: Molecular Mechanism Of Antibiotic Resistancementioning

confidence: 99%

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Analysis of Haemophilus species in patients with respiratory tract infections in Yaoundé, Cameroon

Tchatchouang

Nzouankeu

Hong

et al. 2020

International Journal of Infectious Diseases

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To identifyHaemophilus species and characterise the antimicrobial susceptibility of isolates from patients with respiratory tract infections (RTIs) in Cameroon. Methods: Isolates (n = 95) were from patients with RTIs obtained from two hospitals in Yaoundé, Cameroon. Isolates were identified by biochemical assay, a polymerase chain reaction (PCR)-based method, matrix-assisted laser desorption ionization-time of flight (MALDI-TOF), and whole genome sequencing. Antibiotic minimum inhibitory concentrations were determined by E-test. Results: Haemophilus influenzae (H. influenzae) was the most prevalent species, varying from 76.8 to 84.2% according to the different methods. The isolates were mainly non-typeable (n = 70, 96%). Three H. influenzae isolates were capsulated (b, e and f). The isolates were genetically diverse and 40 unique sequence types were identified, including 11 new ones. Resistance to ampicillin was observed among 52 of 94 (55.3%), and 14 of the 52 (26.9%) produced TEM-1 β-lactamase. PBP3 mutations occurred in 40 of 52 (76.9%) ampicillin-resistant isolates. Eleven isolates were chloramphenicol-resistant, with eight of 10 (80%) producing chloramphenicol acetyltransferase. Four Haemophilus isolates were rifampicinresistant, with two mutations in rpoB gene. Five isolates were ciprofloxacin-resistant and harboured mutations in the quinolone-resistance-determining regions of gyrA and parC genes. Conclusion: TheH. influenzae isolates were highly diverse and showed high levels of antibiotic resistance. H. influenzae serotype b is still circulating in the post-vaccination era.

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Section: Bacterial Growth Dna Preparationmentioning

confidence: 99%

Section: Bacterial Identificationmentioning

confidence: 99%

Section: Molecular Mechanism Of Antibiotic Resistancementioning

confidence: 99%

“…Mutations in the ftsI gene encoding PBP3 were determined using http://pubmlst. org/hinfluenzae/ (Deghmane et al, 2019).…”

Section: Molecular Mechanism Of Antibiotic Resistancementioning

confidence: 99%

See 2 more Smart Citations

Analysis of Haemophilus species in patients with respiratory tract infections in Yaoundé, Cameroon

Tchatchouang

Nzouankeu

Hong

et al. 2020

International Journal of Infectious Diseases

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“…However, public health authorities never reported suspicious clusters of NTHi cases during the observation time. A recent study revealed high diversity of NTHi in France [ 33 ]. Future analyses are needed to detect spatio-temporal clusters that include genomic typing of NTHi.…”

Section: Discussionmentioning

confidence: 99%

Invasive Haemophilus influenzae Infections in Germany After the Introduction of Routine Childhood Immunization, 2001–2016

Takla

Schönfeld

Claus

et al. 2020

Open Forum Infectious Diseases

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Introduction Haemophilus influenzae (Hi) serotype b (Hib) vaccination was introduced in Germany in 1990. This study presents a comprehensive overview on the burden of invasive Hi infections for 2001-2016, including serotype-distribution and ampicillin resistance. Materials and Methods Nationwide data from statutory disease surveillance (2001-2016) were linked with laboratory surveillance data (2009-2016). Besides descriptive epidemiology, statistical analyses included multiple imputation to estimate secular trends. Results In 2001-2016, 4,044 invasive Hi infections were reported. Mean incidence was 3.0 per million inhabitants, higher in males (3.2 vs. 2.9 in females) and in age groups <1 year (15.2) and ≥80 years (15.5). Non-typeable Hi (NTHi) caused 81% (n=1,545) of cases in 2009-2016. Of capsulated cases, 69% were serotype f and 17% serotype b. Of Hib cases eligible for vaccination, 10% (3/29) were fully vaccinated. For 2009-2016, significant increasing trends were observed for NTHi and Hif infections in age groups <5 years and ≥60 years and for ampicillin resistance in NTHi. Conclusions This is one of the most comprehensive Hi data analyses since the introduction of Hib vaccines. NTHi and Hif cause an increasing disease burden among elderly patients and infants. Ampicillin resistance in NTHi must be considered in the treatment of invasive Hi infections.

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Haemophilus influenzae Protein D antibody suppression in a multi‐component vaccine formulation

et al. 2022

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Nontypeable Haemophilus influenzae (NTHi) has emerged as a dominant mucosal pathogen causing acute otitis media (AOM) in children, acute sinusitis in children and adults, and acute exacerbations of chronic bronchitis in adults. Consequently, there is an urgent need to develop a vaccine to protect against NTHi infection. A multi-component vaccine will be desirable to avoid emergence of strains expressing modified proteins allowing vaccine escape. Protein D (PD), outer membrane protein (OMP) 26, and Protein 6 (P6) are leading protein vaccine candidates against NTHi. In pre-clinical research using mouse models, we found that recombinantly expressed PD, OMP26, and P6 induce robust antibody responses after vaccination as individual vaccines, but when PD and OMP26 were combined into a single vaccine formulation, PD antibody levels were significantly lower. We postulated that PD and OMP26 physiochemically interacted to mask PD antigenic epitopes resulting in the observed effect on antibody response. However, column chromatography and mass spectrometry analysis did not support our hypothesis. We postulated that the effect might be in vivo through the mechanism of protein vaccine immunologic antigenic competition. We found when PD and OMP26 were injected into the same leg or separate legs of mice, so that antigens were immunologically processed at the same or different regional lymph nodes, respectively, antibody levels to PD were significantly lower with same leg vaccination. Different leg vaccination produced PD antibody levels quantitatively similar to vaccination with PD alone. We conclude that mixing PD and OMP26 into a single vaccine formulation requires further formulation studies.Nontypeable Haemophilus influenzae (NTHi) has become the most common cause of acute otitis media (AOM) in children in the US, persistent AOM, and recurrent AOM [1][2][3][4][5]. Treatment of AOM in children has an annual cost of over $6 billion in the US [6,7].NTHi also causes acute sinusitis and conjunctivitis in children and adults and acute exacerbations of chronic obstructive pulmonary disease (COPD) in adults [8][9][10][11]. COPD is the third leading cause of death in the US, affecting at least 24 million people, and US

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High diversity of invasive Haemophilus influenzae isolates in France and the emergence of resistance to third generation cephalosporins by alteration of ftsI gene

Abstract: High diversity of invasive Haemophilus influenzae isolates in France and the emergence of resistance to third generation cephalosporins by alteration of ftsI gene

Cited by 44 publications

References 26 publications

Analysis of Haemophilus species in patients with respiratory tract infections in Yaoundé, Cameroon

Analysis of Haemophilus species in patients with respiratory tract infections in Yaoundé, Cameroon

Invasive Haemophilus influenzae Infections in Germany After the Introduction of Routine Childhood Immunization, 2001–2016

Haemophilus influenzae Protein D antibody suppression in a multi‐component vaccine formulation

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