High-efficient nucleic acid separation from animal tissue samples via surface modified magnetic nanoparticles

Li, Peipei; Li, Menghang; Fan, Zhang; Wu, Mengyuan; Jiang, Xiaobin; Ye, Bo; Zhao, Zhenjun; Yue, Dongmei; Fan, Qi; Chen, Hạixia

doi:10.1016/j.seppur.2021.118348

Cited by 17 publications

(9 citation statements)

References 34 publications

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“…Solid-phase extraction based on magnetic materials is the primary choice for high-throughput and automated nucleic acid extraction because it does not rely on centrifuges or other complicated equipment, significantly reduces separation times, and the implementation of automation eliminates the errors that tend to occur in manual operations. Commonly used magnetic particles include silica-coated, amino-coated, carboxyl-coated Fe 3 O 4 or γ-Fe 2 O 3 magnetic particles [ 42 ]. Compared to silica-based solid phase extraction techniques, magnetic particles are easier to manipulate and control with external magnets.…”

Section: Microfluidic Chip and On-chip Nucleic Acid Detectionmentioning

confidence: 99%

Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

Wang

et al. 2023

TrAC Trends in Analytical Chemistry

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Section: Microfluidic Chip and On-chip Nucleic Acid Detectionmentioning

confidence: 99%

Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

Wang

et al. 2023

TrAC Trends in Analytical Chemistry

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“…The magnetic-based isolation approach exploits magnetic particles to extract nucleic acids at the circumstance of external magnetic fields. The commonly utilized magnetic particles include silica-coated, amino-coated, and carboxyl-coated Fe 3 O 4 or γ-Fe 2 O 3 magnetic particles [33][34][35][36]. Compared with silicon-based, solid-phase extraction techniques, a distinct feature of the magnetic particles is ease of manipulation and control using an external magnet.…”

Section: Magnetic-based Strategiesmentioning

confidence: 99%

“…After magnetic separation and blocking, the magnetic nanoparticles and DNA complexes can be used directly for PCR, omitting complex and time-consuming purification and elution operations [35]. The negative carboxyl-coated magnetic nanoparticles are also made to isolate nucleic acids, which are adsorbed to the surface in high concentrations of polyethylene glycol and sodium chloride solutions [36]. Utilizing these surface-modified magnetic beads, DNA extraction is compatible with downstream amplification.…”

Section: Magnetic-based Strategiesmentioning

confidence: 99%

Microfluidics-based strategies for molecular diagnostics of infectious diseases

Wang

Hong

et al. 2022

Military Med Res

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Traditional diagnostic strategies for infectious disease detection require benchtop instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a highly miniaturized, automatic, and integrated technology, are a potential substitute for traditional methods in performing rapid, low-cost, accurate, and on-site diagnoses. Molecular diagnostics are widely used in microfluidic devices as the most effective approaches for pathogen detection. This review summarizes the latest advances in microfluidics-based molecular diagnostics for infectious diseases from academic perspectives and industrial outlooks. First, we introduce the typical on-chip nucleic acid processes, including sample preprocessing, amplification, and signal read-out. Then, four categories of microfluidic platforms are compared with respect to features, merits, and demerits. We further discuss application of the digital assay in absolute nucleic acid quantification. Both the classic and recent microfluidics-based commercial molecular diagnostic devices are summarized as proof of the current market status. Finally, we propose future directions for microfluidics-based infectious disease diagnosis.

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“…CAMNPs proved to be capable of single-strand DNA (ssDNA) capture [34], and the amount of isolated DNA correlated with the content of carboxyl groups on the silica-based NP surface [33]. Li et al [35] fabricated three types of silica- Metal-base interaction, and atomic force.…”

Section: Silica-based Materialsmentioning

confidence: 99%

Solid‐phase extraction methods for nucleic acid separation. A review

Yue

et al. 2021

J of Separation Science

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The separation and purification of biomacromolecules such as nucleic acid is a perpetual topic in separation processes and bioengineering (fine chemicals, biopharmaceutical engineering, diagnostics, and biological characterization). In principle, the solid‐phase extraction for nucleic acid exhibits efficient phase separation, low pollution risk, and small sample demand, compared to the conventional liquid‐phase extraction. Herein, solid‐phase extraction methods are systematically reviewed to outline research progress and explore additional solid‐phase sorbents and devices for novel, flexible, and high‐efficiency nucleic acid separation processes. The functional materials capture nucleic acid, magnetic and magnetic‐free solid‐phase extraction methods, separation device design and optimization, and high‐throughput automatable applications based on high‐performance solid‐phase extraction are summarized. Finally, the current challenges and promising topics are discussed.

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High-efficient nucleic acid separation from animal tissue samples via surface modified magnetic nanoparticles

Cited by 17 publications

References 34 publications

Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

Microfluidics-based strategies for molecular diagnostics of infectious diseases

Solid‐phase extraction methods for nucleic acid separation. A review

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