High‐efficient xylitol production by evolved <i>Candida maltosa</i> adapted to corncob hemicellulosic hydrolysate

Jiang, Xiaolong; He, Peng; Qi, Xiaobin; Lin, Yuping; Zhang, Yongkui; Wang, Qinhong

doi:10.1002/jctb.4924

Cited by 18 publications

(5 citation statements)

References 29 publications

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“…9a, Table 3). As far as we know, the xylitol production here was only slightly lower than that in Jiang’s work, which produced 120 g/L xylitol [39] (Table 3). In their study, however, the fermentation was conducted with a much higher initial inoculation at a lower temperature (30 °C) (Table 3).…”

Section: Resultscontrasting

confidence: 60%

Release of glucose repression on xylose utilization in Kluyveromyces marxianus to enhance glucose-xylose co-utilization and xylitol production from corncob hydrolysate

et al. 2019

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BackgroundLignocellulosic biomass is one of the most abundant materials for biochemicals production. However, efficient co-utilization of glucose and xylose from the lignocellulosic biomass is a challenge due to the glucose repression in microorganisms. Kluyveromyces marxianus is a thermotolerant and efficient xylose-utilizing yeast. To realize the glucose–xylose co-utilization, analyzing the glucose repression of xylose utilization in K. marxianus is necessary. In addition, a glucose–xylose co-utilization platform strain will facilitate the construction of lignocellulosic biomass-utilizing strains.ResultsThrough gene disruption, hexokinase 1 (KmHXK1) and sucrose non-fermenting 1 (KmSNF1) were proved to be involved in the glucose repression of xylose utilization while disruption of the downstream genes of cyclic AMP-protein kinase A (cAMP-PKA) signaling pathway or sucrose non-fermenting 3 (SNF3) glucose-sensing pathway did not alleviate the repression. Furthermore, disruption of the gene of multicopy inhibitor of GAL gene expression (KmMIG1) alleviated the glucose repression on some nonglucose sugars (galactose, sucrose, and raffinose) but still kept glucose repression of xylose utilization. Real-time PCR analysis of the xylose utilization related genes transcription confirmed these results, and besides, revealed that xylitol dehydrogenase gene (KmXYL2) was the critical gene for xylose utilization and stringently regulated by glucose repression. Many other genes of candidate targets interacting with SNF1 were also evaluated by disruption, but none proved to be the key regulator in the pathway of the glucose repression on xylose utilization. Therefore, there may exist other signaling pathway(s) for glucose repression on xylose consumption. Based on these results, a thermotolerant xylose–glucose co-consumption platform strain of K. marxianus was constructed. Then, exogenous xylose reductase and xylose-specific transporter genes were overexpressed in the platform strain to obtain YHY013. The YHY013 could efficiently co-utilized the glucose and xylose from corncob hydrolysate or xylose mother liquor for xylitol production (> 100 g/L) even with inexpensive organic nitrogen sources.ConclusionsThe analysis of the glucose repression in K. marxianus laid the foundation for construction of the glucose–xylose co-utilizing platform strain. The efficient xylitol production strain further verified the potential of the platform strain in exploitation of lignocellulosic biomass.Electronic supplementary materialThe online version of this article (10.1186/s12934-019-1068-2) contains supplementary material, which is available to authorized users.

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Section: Resultscontrasting

confidence: 60%

Release of glucose repression on xylose utilization in Kluyveromyces marxianus to enhance glucose-xylose co-utilization and xylitol production from corncob hydrolysate

et al. 2019

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“…The linear gradient elution profile was 3% A at 0 min, 30% A at 20 min, 35% A at 30 min, 90% A at 40 min, 100% A at 41 min, 100% A at 45 min, 3% A at 46 min, 3% A at 55 min . The glucose and other metabolites were analysed by HPLC using an Aminex HPX‐87H ion‐exchange column (Bio‐Rad, USA) . Samples were centrifuged at 10 000 rpm for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Enhanced 5‐aminovalerate production in Escherichia coli from l‐lysine with ethanol and hydrogen peroxide addition

Cheng

Zhang

Huang

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND: Increasing environmental and energy concerns are pushing the development of a bio-based economy, employing highly reproducible, sustainable and green biomanufacturing methods. Here a potential building block 5-aminovalerate for polymer synthesis has been produced from L-lysine. RESULTS: L-lysine -oxidase from Scomber japonicas was overexpressed in BL21(DE3); a lysine degradation gene was knocked out to strengthen this process in the microbe. The additions of ethanol and hydrogen peroxide significantly enhanced the production of 5-aminovalerate. The recombinant Escherichia coli CJ02 strain was cultured in a medium containing 20 g L −1 glucose, 10 g L −1 L-lysine, 4% (v/v) ethanol and 10 mM H 2 O 2 , producing 5.61 g L −1 5-aminovalerate with a yield of 0.56 g g −1 . Compared with the original producer, this titre represents an 18-fold increase. Excellently, 29.12 g L −1 5-aminovalerate could be achieved with a yield of 0.44 g g −1 in a 5 L fermenter. CONCLUSION: This biotechnological 5-aminovalerate production demonstrates a simple, economic, and green technology to replace the ubiquitous chemical synthesis. More importantly, this strategy of adding ethanol to increase protein expression is not only an efficient process for the production of 5-aminovalerate, but also might be used in the production of other high value-added chemicals.

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“…The most important sugar alcohols could be produced by biotechnology routes, i.e., arabitol, erythritol, mannitol, sorbitol, and xylitol 88 . A vast scientific literature is available reporting the biotechnological production of sweeteners using hemicellulosic and cellulosic hydrolysates, molasses, and crude glycerol as fermentative media 100 …”

Section: Emerging Bio‐based Productsmentioning

confidence: 99%

Brazilian biorefineries from second generation biomass: critical insights from industry and future perspectives

Chandel

Forte

Gonçalves

et al. 2021

Biofuels Bioprod Bioref

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Transitioning from gasoline and petroleum‐based products to biofuels and green chemicals is a paradigm shift that will lead to the development of the bioeconomy. In this sense, the role of biorefineries in countries like Brazil is very important as the country generates a huge amount of second generation (2G) biomass every year and also has an attractive consumable market. However, technological innovations are still required to unleash the fullest potential of biomass conversion to biofuels and biochemicals, although successful examples are already a reality. For example, Amyris Inc. has developed renewable products for cosmetics (squalene), healthcare (artemisinin), and flavors / fragrances, and Suzano Papel e Celulose has developed sustainable technologies for the production of pulp and paper, and lignin‐derived adhesives (Ecolig). First‐generation ethanol in Brazil is an established source of transportation fuel but 2G ethanol production with low production costs is still a challenge at commercial scale, and companies like Raízen and GranBio continue making efforts to improve the economic feasibility of the adopted processes. In this context, the RenovaBio Policy launched by the Federal Brazilian Government in December 2017 aims to boost the production and utilization of biofuels and green chemicals in the country. Considering the matters mentioned above, this paper discusses the Brazilian biorefinery developments, technological advances, and current industrial scenario for the production of biofuels and chemicals. © 2021 Society of Industrial Chemistry and John Wiley & Sons Ltd.

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High‐efficient xylitol production by evolved Candida maltosa adapted to corncob hemicellulosic hydrolysate

Cited by 18 publications

References 29 publications

Release of glucose repression on xylose utilization in Kluyveromyces marxianus to enhance glucose-xylose co-utilization and xylitol production from corncob hydrolysate

Release of glucose repression on xylose utilization in Kluyveromyces marxianus to enhance glucose-xylose co-utilization and xylitol production from corncob hydrolysate

Enhanced 5‐aminovalerate production in Escherichia coli from l‐lysine with ethanol and hydrogen peroxide addition

Brazilian biorefineries from second generation biomass: critical insights from industry and future perspectives

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