High endo-β-1,4-d-glucanase activity in a broad pH range from the alkali-tolerant Nocardiopsis sp. SES28

Walker, Denise S.; Ledesma, Pablo; Delgado, Osvaldo Daniel; Breccia, Javier D.

doi:10.1007/s11274-005-9097-x

Cited by 12 publications

(10 citation statements)

References 12 publications

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“…Another isolate from Argentina, Nocardiopsis sp. SES28 produced cellulases when grown in the presence of CMC (Walker et al 2006). The culture produced the enzyme when it was grown at pH 8.0.…”

Section: Carbohydrases Obtained From Nocardiopsis Speciesmentioning

confidence: 99%

Nocardiopsis species as potential sources of diverse and novel extracellular enzymes

Bennur

Kumar

Zinjarde

et al. 2014

Appl Microbiol Biotechnol

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Members of the genus Nocardiopsis are generally encountered in locations that are inherently extreme. They are present in frozen soils, desert sand, compost, saline or hypersaline habitats (marine systems, salterns and soils) and alkaline places (slag dumps, lake soils and sediments). In order to survive under these severe conditions, they produce novel and diverse enzymes that allow them to utilize the available nutrients and to thrive. The members of this genus are multifaceted and release an assortment of extracellular hydrolytic enzymes. They produce enzymes that are cold-adapted (α-amylases), thermotolerant (α-amylases and xylanases), thermoalkalotolerant (cellulases, β-1,3-glucanases), alkali-tolerant thermostable (inulinases), acid-stable (keratinase) and alkalophilic (serine proteases). Some of the enzymes derived from Nocardiopsis species act on insoluble polymers such as glucans (pachyman and curdlan), keratin (feathers and prion proteins) and polyhydroxyalkanoates. Extreme tolerance exhibited by proteases has been attributed to the presence of some amino acids (Asn and Pro) in loop structures, relocation of multiple salt bridges to outer regions of the protein or the presence of a distinct polyproline II helix. The range of novel enzymes is projected to increase in the forthcoming years, as new isolates are being continually reported, and the development of processes involving such enzymes is envisaged in the future.

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Section: Carbohydrases Obtained From Nocardiopsis Speciesmentioning

confidence: 99%

Nocardiopsis species as potential sources of diverse and novel extracellular enzymes

Bennur

Kumar

Zinjarde

et al. 2014

Appl Microbiol Biotechnol

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“…KNU is several folds higher than the activity of Nocardiopsis sp. SES28 reported earlier (Walker et al 2006). Similarly higher exoglucanse, b-glucosidase, xylanase and glucoamylase enzyme activities were observed in the presence of xylan and cellobiose (Table 2).…”

Section: Acetoin Production -mentioning

confidence: 89%

“…These enzymes include proteases, nucleases, lipases and a variety of enzymes that hydrolyze different types of polysaccharides, such as chitin and cellulose (McCarthy 1987). Nocardiopsis species are mainly studied for the production of alkaline proteases (Moreira et al 2003;Dixit and Pant 2000), endo b-1-4 D-glucanase (Walker et al 2006), keratinolytic enzymes (Mitsuiki et al 2002), xylanase (Tsujibo et al 1990), thermostable amylase (Stamford et al 2001) and chitinase (Tsujibo et al 2003). However, no work has been done on the production of multiple cellulolytic enzymes and their characterization by using this species.…”

Section: Introductionmentioning

confidence: 99%

Production of thermotolerant and alkalotolerant cellulolytic enzymes by isolated Nocardiopsis sp. KNU

Saratale

2011

Biodegradation

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A novel cellulolytic bacterium was isolated from the forest soil of KNU University campus. Through 16S rRNA sequence matching and morphological observation it was identified as Nocardiopsis sp. KNU. This strain can utilize a broad range of cellulosic substrates including: carboxymethyl cellulose (CMC), avicel, xylan, cellobiose, filter paper and rice straw by producing a large amount of thermoalkalotolerant endoglucanase, exoglucanase, xylanase and glucoamylase. Optimal culture conditions (Dubos medium, 37°C, pH 6.5 and static condition) for the maximal production of the cellulolytic enzymes were determined. The activity of cellulolytic and hemicelluloytic enzymes produced by this strain was mainly present extracellularly and the enzyme production was dependent on the cellulosic substrates used for the growth. Effect of physicochemical conditions and metal additives on the cellulolytic enzymes production were systematically investigated. The cellulases produced by Nocardiopsis sp. KNU have an optimal temperature of 40°C and pH of 5.0. These cellulases also have high thermotolerance as evidenced by retaining 55-70% activity at 80°C and pH of 5.0 and alkalotolerance by retaining >55% of the activity at pH 10 and 40°C after 1 h. The efficiency of fermentative conversion of the hydrolyzed rice straw by Saccharomyces cerevisiae (KCTC-7296) resulted in 64% of theoretical ethanol yield.

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“…Cellulase activity was determined according to Walker et al (2006) with modifications. Enzymatic extract was obtained by macerating 0.3 g of fresh weight in liquid nitrogen to which 1.5 mL of 1 M NaCl was added.…”

Section: Biochemical Analysismentioning

confidence: 99%

Physiological and ultrastructural analysis reveal the absence of a defined abscission zone in coffee fruits

et al. 2016

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final maturation stages. In general, physiological differences between maturation stages were observed and can largely explain the different detachment forces required at different stages. In conclusion, there is no clear abscission zone between the peduncle and the coffee fruit cv."Icatu amarelo". Nevertheless, the decrease in the detachment force throughout the maturation process can occur due to the activity of cell wall-degrading enzymes together with increased oxidative stress during the fruit ripening.

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High endo-β-1,4-d-glucanase activity in a broad pH range from the alkali-tolerant Nocardiopsis sp. SES28

Cited by 12 publications

References 12 publications

Nocardiopsis species as potential sources of diverse and novel extracellular enzymes

Nocardiopsis species as potential sources of diverse and novel extracellular enzymes

Production of thermotolerant and alkalotolerant cellulolytic enzymes by isolated Nocardiopsis sp. KNU

Physiological and ultrastructural analysis reveal the absence of a defined abscission zone in coffee fruits

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