2019
DOI: 10.1177/0960327119851254
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High expression of DNA methyltransferase 1 in Kazakh esophageal epithelial cells may promote malignant transformation induced by N-methyl-N′-nitro-N-nitrosoguanidine

Abstract: We examined the role of DNA methyltransferase 1 (DNMT1) in N-methyl- N′-nitro- N-nitrosoguanidine (MNNG)–induced malignant transformation of Kazakh esophageal epithelial (EE) cells to better understand the pathogenesis of esophageal cancer (EC). The 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide method and colony formation assays were performed to determine the MNNG dose for malignant transformation. Colony formation assays showed the effects of different frequencies of MNNG exposure… Show more

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“…The genotoxic dose of MNNG in GES-1 cells was 5 μmol/L, and transformation concentration of MNNG in EC109 cell was 1.5 μmol/L. 77 In our study, the viability of Het-1A-HPV and Het-1A-V cells treated with 1.25 μmol/L and 2.5 μmol/L MNNG for 24 h was between 62 and 80%. To ensure cell viability ranged from 70−80%, 2 μmol/L MNNG was used to induce malignant transformation of cells.…”
Section: Discussionmentioning
confidence: 47%
See 1 more Smart Citation
“…The genotoxic dose of MNNG in GES-1 cells was 5 μmol/L, and transformation concentration of MNNG in EC109 cell was 1.5 μmol/L. 77 In our study, the viability of Het-1A-HPV and Het-1A-V cells treated with 1.25 μmol/L and 2.5 μmol/L MNNG for 24 h was between 62 and 80%. To ensure cell viability ranged from 70−80%, 2 μmol/L MNNG was used to induce malignant transformation of cells.…”
Section: Discussionmentioning
confidence: 47%
“…N -Nitroso compounds are DNA alkylating agents; MNNG, a kind of N -nitroso compounds, could be mutagenesis and carcinogenesis through inducing genotoxic. The genotoxic dose of MNNG in GES-1 cells was 5 μmol/L, and transformation concentration of MNNG in EC109 cell was 1.5 μmol/L . In our study, the viability of Het-1A-HPV and Het-1A-V cells treated with 1.25 μmol/L and 2.5 μmol/L MNNG for 24 h was between 62 and 80%.…”
Section: Discussionmentioning
confidence: 50%