High Expression of Hexokinase Isozyme B During Hepatocarcinogenesis in LEC Rats

Jain, Sadhana; Jain, Suresh; Sugiyama, Toshihiro; Taniguchi, Naoyuki

doi:10.1007/978-4-431-68153-3_36

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…It is well known that liver specific isozymesglucokinase and pyruvate kinase-are decreased during hepatocarcinogenesis. We showed that hexokinase may be used as an enzyme marker of the early stages of liver carcinogenesis (9). Hexokinase activity showed two phases; an increase at 4 weeks and a decrease at 10 weeks followed by an increase peaking at 20 weeks.…”

Section: Expression Of Enzyme Markers During the Hepatocarcinogenesismentioning

confidence: 86%

Sexual dimorphism in LEC rat liver: Suppression of carbonic anhydrase III by copper accumulation during hepatocarcinogenesis

et al. 2011

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We examined age-related changes in the protein expression of carbonic anhydrase III (CAIII) in livers of Long-Evans with a cinnamon-like color (LEC) rats using an agouti color (LEA) rats as controls. The levels of the protein of CAIII in the liver of LEC male rats increased before 20 weeks of age, at the stage of acute hepatitis, and were decreased at 54 weeks of age, while those of CAIII in the liver of LEA male rats were highly expressed at all ages. In the normal LEA rats, CAIII showed sexual dimorphism. The level of CAIII in LEA male rat liver relative to female was four times higher. On the other hand, young LEC rat (at 4-12 weeks) showed a higher protein level of CAIII than LEA rats, and then decreased during development of hepatitis. CAIII mRNA also decreased in the LEC rat liver during hepatocarcinogenesis. The level of CAIII in the tumor region was lower than that in the tumor-free region. Immunohistochemical analysis showed that glutathione S-transferase P (GST-P) was positive and CAIII was negative in the precancerous region. The expression of CAIII was suppressed in cancerous lesions in hepatoma-bearing LEC rat liver compared to uninvolved surrounding tissues. These results indicated that suppression of CAIII accompanied hepatocarcinogenesis and it is a secondary consequence of the high copper levels in the liver.

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Section: Expression Of Enzyme Markers During the Hepatocarcinogenesismentioning

confidence: 86%

Sexual dimorphism in LEC rat liver: Suppression of carbonic anhydrase III by copper accumulation during hepatocarcinogenesis

et al. 2011

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Expression of tumor‐associated protein variants in chemically induced rat hepatomas and transformed rat liver cell lines determined by two‐dimensional electrophoresis

1994

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High resolution two-dimensional electrophoresis (2-DE), using the rat liver as a model, was applied to study hepatocarcinogenesis induced by different chemical carcinogens. Several tumor-associated protein variants were detected by 2-DE in chemically induced rat hepatomas and transformed rat liver cell lines compared to normal rat liver tissue. Proliferation-related protein changes and/or protein alterations due to culture conditions were corrected for by comparison with 2-DE patterns of isolated cells from regenerating liver and short-term cultivated liver cells. Some of the tumor-associated variants were further characterized: (i) By peptide mapping with limited proteolysis we detected clear relationships between several variants. (ii) By studying post-translational modifications phosphorylated and glycosylated variants could be determined. (iii) A tumor-associated protein variant was identified by amino acid analysis and amino acid sequencing.

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Deficiency of β1‐6 N‐Acetylglucosaminyltransferase Involved in the Biosynthesis of Blood Group I Antigen in the Liver of LEC Rats

Gu¹,

Nishikawa

Matsuura³

et al. 1992

Japanese Journal of Cancer Research

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The activities of the β1‐6 and β1–3 N‐acetylglucosaminyltransferases, which synthesize blood group I and i antigens, respectively, were measured in various tissues of hepatitis‐ and hepatoma‐predisposed rats (LEC rats). In LEC rats the β1‐6 N‐acetylglucosaminyltransferase activity was barely detectable in the liver, while substantial enzyme activity was found in other tissues. In the control LEA rats the enzyme was expressed in most tissues, including the liver. Immunochemical studies using a monoclonal antibody which recognizes I antigen indicated that the expression of I antigen was less prominent in hepatocytes of LEC rats than in hepatocytes of LEA rats. The level of β1–3 N‐acetylglucosaminyltransferase activity was constant in most of the tissues during the development. These results indicate that the biosynthesis of I antigen does not occur in the livers of the LEC rats.

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High Expression of Hexokinase Isozyme B During Hepatocarcinogenesis in LEC Rats

Cited by 3 publications

References 17 publications

Sexual dimorphism in LEC rat liver: Suppression of carbonic anhydrase III by copper accumulation during hepatocarcinogenesis

Sexual dimorphism in LEC rat liver: Suppression of carbonic anhydrase III by copper accumulation during hepatocarcinogenesis

Expression of tumor‐associated protein variants in chemically induced rat hepatomas and transformed rat liver cell lines determined by two‐dimensional electrophoresis

Deficiency of β1‐6 N‐Acetylglucosaminyltransferase Involved in the Biosynthesis of Blood Group I Antigen in the Liver of LEC Rats

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