High expression of HSP47 in ulcerative colitis-associated carcinomas: proteomic approach

Araki, Kayo; Mikami, Taisei; Yoshida, Tsutomu; Kikuchi, Motohiro; Sato, Yuichi; Ohishi, Masamichi; Kodera, Yasuhiro; Maeda, Tadakazu; Okayasu, Isao

doi:10.1038/sj.bjc.6605163

Cited by 37 publications

(34 citation statements)

References 21 publications

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“…Fraction 2 on all 2D electrophoregrams was distinctly observed. These data to a certain degree contradict the report about increase of the serpin H1 level in ulcerative colitis-associated carcinomas [51]. However, there are materials stating that seprin Н1 is a biomarker of stromal tumor cells [52] and normal fibroblasts are stained by antibodies to serpin H1 more intensely than cancer cells [53].…”

Section: Serpin H1 In Cultivated Cell Linescontrasting

confidence: 52%

Comparative Proteomic Study of Proteins in Prostate Cancer and Benign Hyperplasia Cells

Шишкин¹,

Kovaleva²,

Ivanov³

2011

J Cancer Sci Ther

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Comparative proteomic studies of proteins in DU-145, PC-3, LNCaP and BPH-1 cultivated cells as well as in prostate tissue samples obtained from patients with benign and malignant tumors showed differences in protein profiles, in particular, high content in tissue samples of four isoforms of transgelin and profilin-1. Increased expression of protein Dj-1 was observed in cancer cells and biopsy samples of prostate cancer as opposed to BPH-1 cells and biopsy samples of tissues with benign hyperplasia. Dramatic reduction of serpin H1 was noted in the range of cell lines: BPH-1 → РС-3 → DU-145 → LNCaP. Thus, the obtained data are indicative of protein Dj-1 and serpin H1 participation in formation of the cancer phenotype in prostate cells.

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Section: Serpin H1 In Cultivated Cell Linescontrasting

confidence: 52%

Comparative Proteomic Study of Proteins in Prostate Cancer and Benign Hyperplasia Cells

Шишкин¹,

Kovaleva²,

Ivanov³

2011

J Cancer Sci Ther

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“…This combined with inherent bio logical variations between patient samples further emphasises the importance of achieving sufficient statis tical power [60,61] . It has already been noted that the concentration of candidate IBD biomarkers may be more concentrated in the intestinal tissue compared to serum, potentially reducing the chance of false discoveries.…”

Section: -Dementioning

confidence: 99%

Current application of proteomics in biomarker discovery for inflammatory bowel disease

Chan¹,

Wasinger²,

Leong³

2016

WJGP

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Recently, the field of proteomics has rapidly expanded in its application towards clinical research with objectives ranging from elucidating disease pathogenesis to discovering clinical biomarkers. As proteins govern and/or reflect underlying cellular processes, the study of proteomics provides an attractive avenue for research as it allows for the rapid identification of protein profiles in a biological sample. Inflammatory bowel disease (IBD) encompasses several heterogeneous and chronic conditions of the gastrointestinal tract. Proteomic technology provides a powerful means of addressing major challenges in IBD today, especially for identifying biomarkers to improve its diagnosis and management. This review will examine the current state of IBD proteomics research and its use in biomarker research. Furthermore, we also discuss the challenges of translating proteomic research into clinically relevant tools. The potential application of this growing field is enormous and is likely to provide significant insights towards improving our future understanding and management of IBD.

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“…HSP47 is a product of the CBP2 gene, located at chromosome 11q13.5 (11), which is a region frequently involved in the biological behaviour of malignant tumours, including oral tongue cancer, nasopharyngeal and esophageal squamous cell carcinoma, lung diseases, pancreatic ductal adenocarcinoma, cervical squamous cell and ulcerative colitis-associated carcinoma (12)(13)(14)(15)(16)(17). As a classical serpin, HSP47 has been proven to exert inhibitory effects on tumour proliferation, invasion and motility (18,19).…”

Section: Hsp47 Is Associated With the Prognosis Of Laryngeal Squamousmentioning

confidence: 99%

HSP47 is associated with the prognosis of laryngeal squamous cell carcinoma by inhibiting cell viability and invasion and promoting apoptosis

et al. 2017

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Abstract. Heat shock protein 47 (HSP47) is a 47 kDa collagen binding protein that has a close relationship with the development and progression of tumours. However, little is known concerning the expression profile of HSP47 in laryngeal squamous cell carcinoma (LSCC) patients and there is still insufficient data concerning the underlying mechanisms. The aim of the present study was to explore the expression of HSP47 in LSCC and provide an overview of its association with tumourigenicity and clinical prognosis. The expression of HSP47 in LSCC and adjacent non-cancerous laryngeal tissues was assessed via western blotting and immunohistochemical studies. The prognostic significance of HSP47 expression was analysed using a Kaplan-Meier survival curve. To investigate the influence of HSP47 on the viability, invasion and apoptosis of a LSCC cell line, we performed an in vitro analysis with plasmid vectors and small interfering RNA (siRNA). Our results showed that HSP47 protein expression in the LSCC tissues was markedly decreased compared to that noted in the adjacent non-cancerous tissues, and low expression of HSP47 was correlated with poor prognosis in LSCC patients. Upregulation of HSP47 via plasmid vectors inhibited the proliferation, reduced the invasive ability, increased the sensitivity to cisplatin chemotherapy, promoted apoptosis, and induced the G1 phase arrest of LSCC cells in vitro. The expression of apoptosis-regulating proteins was also altered when HSP47 was upregulated, involving increased expression of cleaved caspase-7/-8/-9, PARP, and Bax and decreased expression of Bcl-2. Our present data suggest that HSP47 is an important prognostic factor and an attractive therapeutic target in LSCC due to its influence on the biological behaviour of LSCC cells.

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High expression of HSP47 in ulcerative colitis-associated carcinomas: proteomic approach

Cited by 37 publications

References 21 publications

Comparative Proteomic Study of Proteins in Prostate Cancer and Benign Hyperplasia Cells

Comparative Proteomic Study of Proteins in Prostate Cancer and Benign Hyperplasia Cells

Current application of proteomics in biomarker discovery for inflammatory bowel disease

HSP47 is associated with the prognosis of laryngeal squamous cell carcinoma by inhibiting cell viability and invasion and promoting apoptosis

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